TNF-α and TGF-β level after intraoperative allogeneic red blood cell transfusion in orthopedic operation patients

Hassani, Hossein; Khoshdel, Alireza; Sharifzadeh, Seyed Reza; Heydari, Mohammad Foad; Alizadeh, Shaban; Aghideh, Ali Noroozi

doi:10.3906/sag-1508-36

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…Thereupon, in the HRM step, the PCR products are gradually heated, and in the melting temperature, the DNA samples are being denatured, causing fluorescence fading. Finally, a melt curve is drawn according to the fluorescence level as opposed to the temperature that even a single base change will cause alterations in the resultant melting curve (Moradabadi et al, 2017;Federici et al, 2018;Er and Chang, 2012;Montgomery et al, 2010;Norambuena et al, 2009;Hassani et al, 2017;Moonesi et al, 2021).…”

Section: Rapid Detection Of N-ras Gene Common Mutations In Acutementioning

confidence: 99%

Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method

Khosravi

Moonesi

Moradabadi

et al. 2022

Asian Pac J Cancer Prev

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Objective: Acute myeloid leukemia is caused by the clonal proliferation of undifferentiated myeloid hematopoietic precursors. AML prognosis is highly involved in the treatment response and is determined by mutations in several genes such as N-RAS. This study aims to identify the distribution of common N-RAS mutations (codons 12, 13, and 61) in AML patients using the HRM method and confirm this method's efficiency for mutation detection by comparing its results with the sequencing data as the Gold standard method. Methods: Peripheral blood samples were taken from 50 newly diagnosed AML patients. Mononuclear cells were isolated from samples, and DNA was extracted. Then, mutation detection was investigated using the HRM method. Efficacy of the HRM method in mutation detection was determined in comparison with direct sequencing. Results: N-RAS mutations were detected in 7 of the 50 samples (14%). Most of the mutations were found in codon 12 (57.14%), and 28.57% and 14.28% of mutations were in codons 61 and 13, respectively. There was no statistically significant association between patients' demographic data and HRM results. Conclusion: According to mutation detection results and the HRM results confirmation with the sequencing method, this method can be introduced as an efficient, low-cost, and fast method for detecting common mutations.

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Section: Rapid Detection Of N-ras Gene Common Mutations In Acutementioning

confidence: 99%

Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method

Khosravi

Moonesi

Moradabadi

et al. 2022

Asian Pac J Cancer Prev

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Impact of transfusion of blood components on the recipient immune system

et al. 2021

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Transfusions of blood provide essential therapeutic measures in a number of pathological conditions. However, when carrying out blood component therapy, it is important to consider probability of post-transfusion complications. Most of them are immune-mediated side effects. The unfavorable consequences of blood transfusions can manifest at long-range time periods, and pathogenesis of these phenomena may be associated not only with the presence of alloantibodies. They may be caused by alloimmunization to HLA antigens, leukocyte factors, including cytokines, products of leukocyte degranulation, as well as storage-related erythrocyte damage («storage lesion»), immunomodulatory properties of extracellular vesicles or microparticles derived from blood components, and other factors. Despite significant number of publications on this issue, a lot of unresolved issues still remain, concerning transfusion-related effects of blood components on the immune system of recipients. The review article provides the results of current studies in this area. We present and discuss the results of current studies and the features of transfusion-mediated immunomodulation (TRIM) revealed over recent years, when transfusing different blood components. The role of plasma factors, microparticles, platelets and erythrocytes, HLA sensitization and microchimerism in the development of TRIM is highlighted, the data on occurrence and clinical features of TRIM in perioperative period are presented. A separate section of the review provides information about recent clinical studies, devoted to the issues of TRIM in different clinical cohorts, including newborns, patients with malignant neoplasms, immunocompromised patients after heart and vascular surgery. The data on TRIM incidence in the patients with exhausted immune system due to previous disease or treatment, severe comorbidity, extensive surgical thoracic/abdominal intervention and artificial circulation are also in scope. As based on the studies performed, the role of distinct measures, e.g., washing of erythrocyte concentrates, leukodepletion, and gamma irradiation are discussed in view of potential TRIM prevention. The results of published research do not allow us to draw definite conclusions about the effects of blood component transfusion on the immune system of recipients with respect to differences between the studied groups of patients, characteristics of the studied disorders and clinical situations, diversity of hemocomponents, as well as varying standards of transfusion therapy adopted in different countries. However, the systematic literature review may provide some guidance in transfusion-mediated immune modulation.

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TNF-α and TGF-β level after intraoperative allogeneic red blood cell transfusion in orthopedic operation patients

Cited by 2 publications

References 20 publications

Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method

Rapid Detection of N-RAS Gene Common Mutations in Acute Myeloid Leukemia (AML) Using High Resolution Melting (HRM) Method

Impact of transfusion of blood components on the recipient immune system

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