TNF-α, HGF and TGF-β1 are Involved in Liver Regeneration Following Partial Hepatectomy Using Portal Vein Arterializations

Abstract

Kratak sadr`aj: Eksperimenti vezani za regeneraciju jetre posle parcijalne hepatektomije pokazali su da TNF-a, HGF i TGF-b1 kao i drugi citokini igraju va`ne uloge u razli~itim faza ma regeneracije jetre, me|utim, uticaj arterijalizacije port ne vene (APV) na ekspresiju ovih citokina tokom regene racije jetre nije jasan. Sprague Dawley pacovi nasumi~no su podeljeni u APV grupu i kontrolne grupe, a krv za detekciju ALT uzeta je pomo}u automatskog biohemijskog analiza tora. Ekspresije TNF-a, HGF i TGF-b1 u tkivima jetre detektovane su kvantitativnom RT-PCR. Nivoi ALT u obe grupe u po~etnom periodu posle operacije bili su zna~ajno vi{i nego pre hirur{kog za hvata i postepeno su se normalizovali posle 7 dana od operacije. Posle 12 h i 24 h od zahvata, ekspresija TNF-a u APV grupi bila je zna~ajno vi{a nego u kontrolnoj grupi (P<0,05), ali posle 7 dana od operacije nije uo~ena zna~ajna razlika izme|u dve grupe. Ekspresija HGF u APV grupi posle 12 h bila je sli~na onoj u kontrolnoj grupi, ali je posle 24 h bila zna~ajno vi{a nego u kontrolnoj grupi (P<0,05). Posle 24 h, ekspresija TGF-b1 u APV grupi bila je zna~ajno ni`a nego u kontrolnoj grupi (P<0,05), ali posle 48 h od operacije izme|u dve grupe nije otkrivena zna~ajna razlika. Pozitivan uticaj na arterija li zaciju portne vene u ranim fazama regeneracije jetre bio je povezan s promenama u ekspresijama TNF-a, HGF i TGF-b1. Summary: Experiments on liver regeneration after partial hepatectomy have shown that TNF-a, HGF and TGF-b1 and other cytokines play important roles in the different stages of liver regeneration, however, the effect of portal vein arteria liz ation (PVA) on the expressions of these cytokines during liver regeneration is not clear. Sprague Dawley rats were randomly divided into the PVA group and control groups, and blood was collected for the detection of ALT using an automatic bio chemical analyzer. The expressions of TNF-a, HGF and TGF-b1 in liver tissues were detected by quan titative RT-PCR. The ALT levels in both groups in the early period after surgery were significantly higher than those before operation, and gradually returned to normal at 7 days after surgery. At 12 h and 24 h after operation, the TNF-a expres sion in the PVA group was significantly higher than that in the control group (P<0.05), but no significant dif fe rence at 7 days after sur gery was observed between the two groups. At 12 h, the HGF expression in the PVA group was similar to that in the con trol group, but significantly higher than in the control group at 24 h (P<0.05). At 24 h, the TGF-b1 expres sion in the PVA group was sig nificantly lower than that in the control group (P <0.05), but no significant difference was found at 48 h after surgery between the two groups. The promo tive effects on the portal vein arterialization at the early stage of liver rege neration were associated with the changes in the expressions of TNF-a, HGF and TGF-b1.

Liver dual arterial blood supply maintains liver regeneration: Analysis of signaling pathways in rats

“…Previous work from our group suggested that the mechanisms behind LDABS-mediated liver regeneration differ from regeneration after PH alone. Specifically, fluorescent quantitative RT-PCR in our previous study revealed differential expression of TNF-α, HGF and TGF-β1 in the liver of rats with PH alone vs. with PH plus LDABS ( 21 ).…”

Effects of dual arterial blood supply on liver regeneration in the graft and the host following heterotopic auxiliary liver transplantation

“…The animal models in groups A and B were prepared based on methods previously described ( 4 , 7 ). The animal model in group C was established on the basis of an improvement of the methods described by Schleimer et al ( 8 ).…”

Portal vein arterialization as a salvage procedure in hepatopancreatobiliary surgery: a systematic review

“…44 Higher rates of remnant liver regeneration and hepatocyte proliferation were reported in liver resection in PVA models than in models without ar terial supply (control group). [45][46][47][48][49] Moreover, cholestasis, bile duct injury, inflammation, energy state due to elevation in ATP content, hypoxia and hypoxia-associated gene changes were reported to be decreased with PVA compared to liver de-arterialization without reconstruction. 50,51 The hepatic artery provides the main oxygen supply to the biliary tract.…”