TNF-α is a novel target of miR-19a

Liu, Mei; Wang, Zaozao; Yang, Shangbin; Zhang, Wei; He, Shasha; Hu, Chenfei; Zhu, Hongxia; Quan, Lanping; Bai, Jinfeng; Xu, Ningzhi

doi:10.3892/ijo.2011.924

Cited by 47 publications

(42 citation statements)

References 24 publications

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“…This suggested that miR-19a was essential for TNF-α-mediated EMT and provided mechanistic insight into the role of miR-19a in mediating some of the TNF-α mediated EMT activity. Considering, TNF-α has been reported previously to be a miR-19a gene target in ESCC34 and ulcerative colitis35, we had conducted the western blot assay to determine whether TNF-α would be a target in CRC cells. Ectopic miR-19a expression decreased the expression of TNF-α in both HCT 116 and Caco-2 cells (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer

Huang

Wang

Wen

et al. 2015

Sci Rep

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Lymph node metastasis is an important factor determining the outcome of colorectal cancer. Although epithelial-to-mesenchymal transition (EMT), TNF-α and microRNA (miRNA) have been found to play important roles in lymph node metastasis, the underlying molecular mechanism remains unclear. Here we reported that high expression of microRNA-19a (miR-19a) was associated with lymph node metastasis and played an important role in TNF-α-induced EMT in colorectal cancer (CRC) cells. We analyzed miR-19a expression in surgical tissue specimens from 11 CRC patients and 275 formalin-fixed, paraffin-embedded CRC patients. We found that miR-19a was up-regulated in CRC tissues and high expression of miR-19a was significantly associated with lymph node metastasis. We further analyzed miR-19a lymph node metastasis signature in an external validation cohort of 311 CRC cases of the TCGA. MiR-19a was found to be significantly associated with lymph node metastasis in rectal cancer. In vitro, we showed that overexpression of miR-19a in human CRC cell lines promoted cell invasion and EMT. Furthermore, miR-19a was up-regulated by TNF-α and miR-19a was required for TNF-α-induced EMT and metastasis in CRC cells. Collectively, miR-19a played an important role in mediating EMT and metastatic behavior in CRC. It may serve as a potential marker of lymph node metastasis.

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Section: Discussionmentioning

confidence: 99%

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer

Huang

Wang

Wen

et al. 2015

Sci Rep

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“…miRNA-19b is one of the major members of the miRNA-17-92 family (8). It has been found that miR-19b is highly expressed in breast, cervical, colon and pancreatic carcinomas and participates in the apoptosis of tumor cells (9,10). …”

Section: Introductionmentioning

confidence: 99%

Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma

et al. 2017

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The effects of naringin on the expression of miR-19b and cell apoptosis were investigated in the human hepatocellular carcinoma cell line HepG2. HepG2 cells were treated with varied concentrations of naringin. The effects of naringin on the proliferation of HepG2 cells were observed by an MTT assay, morphological changes of cells were observed by an inverted microscope, cell apoptosis was detected by DAPI staining, miR-19b mRNA levels were determined with RT-PCR, and the expression of Bax and Bcl-2 proteins was examined by western blot assay. MTT results showed that naringin significantly inhibited the proliferation of HepG2 cells. Apoptotic HepG2 cells showed obvious changes in morphology under inverted microscope. DAPI staining suggested that naringin could induce cell shrinkage and nuclear chromatin condensation. RT-PCR results showed that naringin could upregulate the expression of miR-19b mRNA. Finally, western blot suggested that naringin upregulated the expression of Bax protein, but downregulated the expression of Bcl-2 protein. In conclusion, naringin can upregulate the expression of miR-19b mRNA and induce HepG2 cell apoptosis. In addition, it can also upregulate the expression of Bax protein and downregulate the expression of Bcl-2 protein during the process of apoptosis.

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“…In addition, we found 2 members ( miR-17-5p , miR-20a ) of miR-17-92 cluster only appeared in the subtype 1. MiR-17-92 cluster which locates on the C13orf25 gene is overexpressed in ESCC and usually promote cancer cell proliferation [38]. Then we validated the expression of these two clusters in an independent data set (detail in Methods), and found miR-17-5p , miR-20a and miR-16-5p were upregulated in ESCC.…”

Section: Resultsmentioning

confidence: 99%

Construction of differential mRNA-lncRNA crosstalk networks based on ceRNA hypothesis uncover key roles of lncRNAs implicated in esophageal squamous cell carcinoma

et al. 2016

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Increasing evidence has indicated that lncRNAs acting as competing endogenous RNAs (ceRNAs) play crucial roles in tumorigenesis, metastasis and diagnosis of cancer. However, the function of lncRNAs as ceRNAs involved in esophageal squamous cell carcinoma (ESCC) is still largely unknown. In this study, clinical implications of two intrinsic subtypes of ESCC were identified based on expression profiles of lncRNA and mRNA. ESCC subtype-specific differential co-expression networks between mRNAs and lncRNAs were constructed to reveal dynamic changes of their crosstalks mediated by miRNAs during tumorigenesis. Several well-known cancer-associated lncRNAs as the hubs of the two networks were firstly proposed in ESCC. Based on the ceRNA mechanism, we illustrated that the“loss” of miR-186-mediated PVT1-mRNA and miR-26b-mediated LINC00240-mRNA crosstalks were related to the two ESCC subtypes respectively. In addition, crosstalks between LINC00152 and EGFR, LINC00240 and LOX gene family were identified, which were associated with the function of “response to wounding” and “extracellular matrix-receptor interaction”. Furthermore, functional cooperation of multiple lncRNAs was discovered in the two differential mRNA-lncRNA crosstalk networks. These together systematically uncovered the roles of lncRNAs as ceRNAs implicated in ESCC.

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TNF-α is a novel target of miR-19a

Cited by 47 publications

References 24 publications

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer

Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer

Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma

Construction of differential mRNA-lncRNA crosstalk networks based on ceRNA hypothesis uncover key roles of lncRNAs implicated in esophageal squamous cell carcinoma

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