TNF-α promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines

A transgenic mouse model for the in vivo bioluminescence imaging of the expression of the lysophosphatidic acid receptor 3: relevance for inflammation and uterine physiology research

“…Important roles for LPA receptors in the nervous system, cancer progression and reproduction have been elucidated by analyzing knock-out mice for the genes encoding these receptors (Anliker and Chun 2004;Contos et al 2000;Ishii et al 2004). In particular, knock-out mice for Lpar3 not only revealed the importance of this receptor in embryo implantation and spacing (Ye et al 2005) but also in the recruitment of leukocytes to the site of inflammation (Zhao et al 2011). LPA-induced chemokine secretion (Zhao et al 2008) and leukocyte recruitment (Zhao et al 2011) was shown to be enhanced by TNF-a.…”

“…In particular, knock-out mice for Lpar3 not only revealed the importance of this receptor in embryo implantation and spacing (Ye et al 2005) but also in the recruitment of leukocytes to the site of inflammation (Zhao et al 2011). LPA-induced chemokine secretion (Zhao et al 2008) and leukocyte recruitment (Zhao et al 2011) was shown to be enhanced by TNF-a.…”

Lysophosphatidic Acid (LPA) Signaling and Cardiovascular Pathology

“…LPA also elicits the release of IL-6 from SMCs (54). In an air pouch model, LPA recruits both neutrophils and monocytes, triggers the release of IL-6 and KC, and enhances TNFα effects in an LPA 1 -and LPA 3 -dependent manner (55). Together, these results focus attention on LPA 1 and LPA 3 as potentially important mediators of vascular inflammation.…”

“…To our knowledge, the effects of LPA in eliciting peritonitis or dermatitis has not been examined. However, as discussed above, LPA does trigger neutrophil and monocyte migration into air pouches (55). In the air pouch model, 3 mL of sterile air is injected subcutaneously twice, 72 hours apart.…”

Sphingosine-1-Phosphate and Rheumatoid Arthritis: Pathological Implications and Potential Therapeutic Targets

“…Since immune cells express a wide repertoire of chemokine receptors, including those of IL-8, MCP-1, SDF-1α, IP-10 and RANTES [65], S1P may indirectly drive the recruitment and retention of inflammatory cells in RA by this chemokine secretion. Indeed, a bioactive lipid structurally-related to S1P, lysophosphatidic acid (LPA), is able to recruit leukocytes into an in vivo inflammatory site by stimulating inflammatory cytokine/chemokine secretion [66].…”