TNFα, interleukin-10 and interleukin-18 expression in cells of the bronchoalveolar lavage in patients with pulmonary complications following bone marrow or peripheral stem cell transplantation: a preliminary study

Hauber, Hans-Peter; Mikkilä, A; Erich, JM; Kröger, N.; Meyer, Andreas; Schoder, Volker; Zander, AR; Pforte, A

doi:10.1038/sj.bmt.1703722

Cited by 23 publications

(19 citation statements)

References 24 publications

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“…A study compared HSCT recipients who had infectious pneumonia (n514) to another group with idiopathic pneumonia syndrome or BO (n56). The level of tumour necrosis factor (TNF)-a in the BAL fluid was significantly higher in the latter group [49]. Higher levels of TNF-a were associated with a worse outcome.…”

Section: Bronchoscopymentioning

confidence: 78%

“…These inflammatory reactions are characterised by an increase in inflammatory cytokines, such as interleukin (IL)-1, IL-6, IL-8, IL-18 and TNF-a [68,69]. In one study [49], 11 patients with pulmonary complications following allogeneic HSCT (six patients had idiopathic pneumonia syndrome and/or BO) who had BAL fluid analysis were compared with 11 healthy volunteers. The HSCT recipients with BO had significantly higher levels of lavage fluid TNF-a and IL-18 compared with the controls.…”

Section: Pathogenesismentioning

confidence: 99%

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Bronchiolitis obliterans following haematopoietic stem cell transplantation

Soubani

Uberti²

2007

Eur Respir J

111

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The aim of the present article is to review the available clinical data on bronchiolitis obliterans following haematopoietic stem cell transplantation (HSCT).The data sources used were the Medline database and references from the identified articles related to bronchiolitis obliterans, noninfectious pulmonary complications and HSCT.HSCT is an important treatment for a variety of malignant and nonmalignant conditions. However, the procedure is limited by significant complications that may involve every organ of the body. Pulmonary complications are seen in 40-60% of HSCT recipients. The recent advances in prophylaxis and treatment of infectious complications have increased the significance of late noninfectious pulmonary conditions.Currently, bronchiolitis obliterans is one of the most challenging pulmonary complications facing clinicians who are taking care of haematopoietic stem cell transplantation recipients. This article reviews the clinical and pathological features of this condition, sheds some light on potential mechanisms of pathogenesis, and discusses the available management options.

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Section: Bronchoscopymentioning

confidence: 78%

Section: Pathogenesismentioning

confidence: 99%

Bronchiolitis obliterans following haematopoietic stem cell transplantation

Soubani

Uberti²

2007

Eur Respir J

111

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“…[13][14][15]17,21,22,28 In particular, TNF-␣ is increased in the BAL fluid during experimental and clinical IPS. 21, 35 Neutralization of TNF-␣ significantly reduces the severity of lung injury after BMT in mice, 17 and similar therapy in humans has been associated with improvements in pulmonary dysfunction. 7 However, the cellular source and whether donor-or host-derived TNF-␣ is critical in the development of IPS have not been fully examined.…”

Section: Discussionmentioning

confidence: 99%

Donor-derived TNF-α regulates pulmonary chemokine expression and the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation

Hildebrandt

Olkiewicz

Corrion

et al. 2004

Blood

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Idiopathic pneumonia syndrome (IPS) is a significant cause of mortality after allogeneic bone marrow transplantation (allo-BMT), and tumor necrosis factor-␣ (TNF-␣) is a significant effector molecule in this process. However, the relative contribution of donor-versus host-derived TNF-␣ to the development of IPS has not been elucidated. Using a lethally irradiated parent 3 F1 mouse IPS model, we showed that 5 weeks after transplantation allo-BMT recipients developed significant lung injury compared with syngeneic controls, which was associated with increased bronchoalveolar lavage (BAL) fluid levels of TNF-␣, elevated numbers of donorderived TNF-␣-secreting T cells, and increased pulmonary macrophage production of TNF-␣ to lipopolysaccharide (LPS) stimulation. Allo-BMT with TNF-␣ ؊/؊ donor cells resulted in significantly reduced IPS severity, whereas utilization of TNF-␣-deficient mice as BMT recipients had no effect on IPS. We next determined that TNF-␣ secretion from both donor accessory cells (monocytes/macrophages) and T cells significantly contributed to the development of IPS. Importantly, the absence of donor T-cell-derived TNF-␣ resulted in a significant decrease in inflammatory chemokine production in the lung and near complete abrogation of IPS.Collectively, these data demonstrate that donor TNF-␣ is critical to the development of IPS and reveal a heretofore unknown mechanism for T-cell-derived TNF-␣ in the evolution of this process.

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“…RNA preparation, reverse transcription and PCR were performed as previously described [4]. The oligonucleotide primers for human g-actin, IL-18 and IL-10 have been published before [5,6].…”

Section: Rt-pcr and Semiquantitative Pcr Analysismentioning

confidence: 99%

Decreased interleukin-18 expression in BAL cells and peripheral blood mononuclear cells in adult cystic fibrosis patients

Hauber

Beyer

Meyer

et al. 2004

Journal of Cystic Fibrosis

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Lymphocytes from cystic fibrosis (CF) patients secrete less interferon-gamma (IFN-gamma) upon stimulation compared to controls. Expression of interleukin (IL)-18 as an IFN-gamma inducing factor and of IL-10 as an IL-18 inhibiting factor were determined in bronchoalveolar lavage (BAL) cells from CF patients (n=5) and from normal control subjects (n=9) as well as in peripheral blood mononuclear cells (PBMC) from patients (n=12) and from control subjects (n=9) with RT-PCR. IL-18 and IL-10 serum protein levels were measured using ELISA. BAL cells and PBMC of CF patients expressed significantly less IL-18 compared to controls (p<0.05). There was no significant difference for IL-10 in BAL cells. However, PBMC from patients expressed significantly more IL-10 mRNA (p<0.05). IL-18 serum protein levels were decreased in the patient group, whereas IL-10 serum concentrations were elevated. Stimulation with rhIL-10 reduced IL-18 expression in PBMC from CF patients. Decreased IL-18 expression in CF patients may contribute to decreased IFN-gamma production. IL-10 may contribute to inhibit IL-18 expression in PBMC in CF.

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TNFα, interleukin-10 and interleukin-18 expression in cells of the bronchoalveolar lavage in patients with pulmonary complications following bone marrow or peripheral stem cell transplantation: a preliminary study

Cited by 23 publications

References 24 publications

Bronchiolitis obliterans following haematopoietic stem cell transplantation

Bronchiolitis obliterans following haematopoietic stem cell transplantation

Donor-derived TNF-α regulates pulmonary chemokine expression and the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation

Decreased interleukin-18 expression in BAL cells and peripheral blood mononuclear cells in adult cystic fibrosis patients

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