To Evaluate Cytotoxicity of Resin-based
Restorative Materials on Human Lymphocytes by
Trypan Blue Exclusion Test: An in vitro Study

Srivastava, Vinay Kumar; Singh, Rajat Kumar; Malhotra, Sonia; Singh, Aditi

doi:10.5005/jp-journals-10005-1070

Cited by 6 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study showed that Medental micro-hybrid composite resins elicited a slightly lower cellular inflammatory response on the 2nd and 14th days, but the vascular congestion was slightly higher on the 2nd day of implantation. As reported in culture experiments, 11,12 the difference in the cellular inflammatory responses may be related to the higher filler content in the Medental product. Ergun et al 11 discovered that the composite resins that had the highest rate of survival among the five tested composite resins was the one with the highest weight (87%) of filler content.…”

Section: Discussionmentioning

confidence: 70%

“…Ergun et al 11 discovered that the composite resins that had the highest rate of survival among the five tested composite resins was the one with the highest weight (87%) of filler content. Srivastava et al 12 also reported that flowable composite resins with higher resin content showed maximum toxicity compared to nano composite resin and compomer.…”

Section: Discussionmentioning

confidence: 98%

“…7-9 Methacrylate-based composite resins are believed to release uncured monomers into the tissues, which have been associated with hypersensitivity, cytotoxicity, genotoxicity, estrogencity and immune system alterations. 10 Reports from in vitro studies 11,12 revealed a direct association between resin content and cytotoxicity, but implantation studies in this regard are sparse. It is necessary to investigate this parameter in animal studies because the results obtained from different techniques were found to be inconsistent.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Relative biocompatibility of micro-hybrid and nano-hybrid light-activated composite resins

Arigbede¹,

Adeyemi²,

Femi-Akinlosotu³

2017

J Dent Res Dent Clin Dent Prospects

View full text Add to dashboard Cite

Background. In vitro studies have revealed a direct association between resin content and cytotoxicity of composite resins; however, implantation studies in this regard are sparse. This study investigates the relationship between filler content of composite resins and biocompatibility.Methods. This research employed twelve 180‒200-gr male Wistar rats, 1 nano-hybrid (Prime-Dent Inc.) and 1 micro-hybrid (Medental Inc.) composite resins containing 74% and 80‒90% filler content, respectively. The samples were assessed on the 2nd, 14th and 90th day of implantation. Four rats were allocated to each day in this experimental study. A section of 1.5mm long cured nano-hybrid and micro-hybrid materials were implanted into the right and left upper and lower limbs of the rats, respectively. Eight samples were generated on each day of observation. Inflammation was graded according to the criteria suggested by Orstavik and Major. Pearson’s chi-squared test was employed to determine the relationship between the tissue responses of the two materials. Statistical significance was set at P < 0.05.Results. The average grade of inflammation for the nano-hybrid on the 2nd day of implantation was 3.3. The micro-hybrid resin had a score of 3.0 for cellular inflammation. On the 14th day, the micro-hybrid resin also exhibited a lower average grade for cellular inflammation. On the 90th day, the micro-hybrid resin had a higher grade of inflammation (0.9) compared to 0.3 recorded for nano-hybrid. The composite resins with higher filler content elicited a significantly lower grade of inflammation irrespective of the duration (χ=20.000, df=8, P=0.010) while the composite resins with lower filler content elicited a significantly lower inflammatory response on the 90th day (χ=4.000, df=1, P=0.046).Conclusion. The composite resins with higher filler content generally elicited significantly lower grades of inflammation, and the composite resins with lower filler content exhibited significantly lower inflammatory response on the 90th day of implantation.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relative biocompatibility of micro-hybrid and nano-hybrid light-activated composite resins

Arigbede¹,

Adeyemi²,

Femi-Akinlosotu³

2017

J Dent Res Dent Clin Dent Prospects

View full text Add to dashboard Cite

show abstract

“…To identify the rate of living cells in the culture, different stains (trypaneblue, erythrosine, naphthalene black, diacetyl fluorescent or neutral red) are used which can enter the cell structure. In the evaluation of metabolic impairments, MTT, LDH and Alamar blue tests are primarily used [51][52][53].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Dental Adhesives

Yıldırım¹

2017

ADOH

View full text Add to dashboard Cite

The clinical success of dental materials is dependent not only on physical and chemical properties, but also on biological reliability. Materials containing resin, which are used in restorative dentistry, have the potential to create local or systemic reactions on tissues such as the pulp, gingiva and oral mucosa. When adhesive resins are applied directly on the pulp, infection or necrosis generally occurs. This is because the organic matrix of the adhesives contains components which may have a cytotoxic effect. These components have also been reported to cause allegic skin problems in the patient or in dentistry personnel. In recent years, resin monomers have been reported to lead to the formation of tumours in the saliva glands and toxicity in renal cells.With some clinical precautions it is possible to reduce the direct biological risks which could be caused by materials containing resin. The selection of adhesive materials taking into consideration the monomer content and the cytotoxic pH range, is a factor which could reduce the possibility of cytoxicity.

show abstract

“…Srivastava et al 53 evaluated the cytotoxicity of nanocomposites, flowable composites, and composite materials on human lymphocyte cells. They found that all three materials showed cytotoxic effects, and flowable composites had a higher cytotoxic effect than composites and nanocomposites.…”

Section: Cytotoxicity Studies On Resin-containing Materialsmentioning

confidence: 99%

An overview to biocompatibility of resin based restorative materials

Avcılar,

Bakır,

Bakır

2023

JDSE

View full text Add to dashboard Cite

Nowadays, resin based materials find a wide range of use in dentistry due to their aesthetic properties, mechanical durability and cost advantages. Dentistry materials; They can have an effect because they are in direct contact with various tissues such as gums, tongue, lips and cheeks, in addition to periodontium, pulp, dentin and enamel. It is important that resin materials produced with new technologies to be used in restorative dentistry not only have mechanical, physical, functional and aesthetic properties, but also be carefully evaluated in terms of biological compatibility. The purpose of this review is to review the basic concepts and methods related to biocompatibility, to present data from studies on the cytotoxicity of resin-based materials, and finally to make recommendations for clinical applications.

show abstract

To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study

Cited by 6 publications

References 7 publications

Relative biocompatibility of micro-hybrid and nano-hybrid light-activated composite resins

Relative biocompatibility of micro-hybrid and nano-hybrid light-activated composite resins

Biocompatibility of Dental Adhesives

An overview to biocompatibility of resin based restorative materials

Contact Info

Product

Resources

About