To explore the regulatory effect of Buyang Huanwu Decoction on cerebral infarction based on quantitative proteomics

Liu, Ying; Miao, Lan; Guo, R. S.; Li, He; Sun, Mingqian; Pan, Yinghong; Lin, Li; Ren, Junguo; Liu, Jianxun

doi:10.1016/j.jprot.2023.104850

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…While pharmacological approaches are pivotal in stroke management, the utilization of traditional Chinese medicine (TCM) has garnered significant attention. Numerous TCM formulations, such as Sanhuang Xiexin decoction, [ 26 ] Xinglou Chengqi decoction, [ 27 ] and Buyang Huanwu Decoction, [ 28 ] have displayed clinical efficacy in stroke treatment. Specifically, LZD has demonstrated favorable outcomes in ameliorating post-stroke cognitive impairment and hemiplegia.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms underlying the therapeutic effects of Linggui Zhugan decoction in stroke: Insights from network pharmacology and single-cell transcriptomics analysis

Sun,

Luo,

Fang

et al. 2024

Medicine

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Linggui Zhugan decoction (LZD), a traditional Chinese medicine formula, has demonstrated significant therapeutic effects in managing poststroke cognitive impairment and hemiplegia. However, the precise molecular mechanisms underlying its efficacy remain incompletely elucidated. The active ingredients and target proteins of LZD were retrieved from the traditional Chinese medicine systems pharmacology database and analysis platform database, which is specifically designed for traditional Chinese medicine research. The stroke-related genes were obtained from publicly available databases. Protein–protein interaction, enrichment analysis, and single-cell data analysis were conducted to identify key cells, targets, and pathways. Molecular docking was employed to assess the binding affinity between key components and targets. Network pharmacology analysis identified 190 active ingredients and 248 targets in LZD. These targets were significantly enriched in processes and pathways such as cellular response to lipid, orexin receptor pathway, and were significantly associated with Cerebral infarction and Middle Cerebral Artery Occlusion. Intersection analysis with 2035 stroke-related genes revealed 144 potential targets, which exhibited 2870 interactions and were significantly enriched in signaling pathways such as PI3K-AKT single pathway, MAPK single pathway, and tumor necrosis factor single pathway. Gene set variation analysis showed that the targets of LZD exhibited higher enrichment scores in microglia, M2 macrophages, endothelial cells, and neutrophils, while lower enrichment scores were observed in oligodendrocytes. Furthermore, molecular docking demonstrated a strong binding affinity between key active ingredients and targets. Network pharmacology and single-cell sequencing analysis elucidated the key cells, pathways, targets, and components involved in the therapeutic mechanism of LZD for the treatment of stroke.

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Section: Discussionmentioning

confidence: 99%

Molecular mechanisms underlying the therapeutic effects of Linggui Zhugan decoction in stroke: Insights from network pharmacology and single-cell transcriptomics analysis

Sun,

Luo,

Fang

et al. 2024

Medicine

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“…[99,100] A double-blind, randomized controlled trial has proved that Buyang Huanwu Decoction could reduce the nerve damage. [101] Buyang Huanwu Decoction affects the inflammatory response, protecting the nerves, and fighting apoptosis. [102] It decreases TNF-α and inflammatory cells in the ischemic penumbra, inducing the differentiation potential of neural stem cells to neurons.…”

Section: Buyang Huanwu Decoctionmentioning

confidence: 99%

“…[99,100] A double-blind, randomized controlled trial has proved that Buyang Huanwu Decoction could reduce the nerve damage. [101]…”

Section: The Role Of the Tcm For Blood Circulation And Stasis Removal...mentioning

confidence: 99%

The role of JAK/STAT signaling pathway in cerebral ischemia-reperfusion injury and the therapeutic effect of traditional Chinese medicine: A narrative review

Song,

Zhang,

Zhu

et al. 2023

Medicine

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Cerebral ischemia is a cerebrovascular disease with symptoms caused by insufficient blood or oxygen supply to the brain. When blood supplied is restored after cerebral ischemia, secondary brain injury may occur, which is called cerebral ischemia-reperfusion injury (CIRI). In this process, the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway plays an important role. It mediates neuroinflammation and participates in the regulation of physiological activities, such as cell proliferation, differentiation, and apoptosis. After CIRI, M1 microglia is activated and recruited by the damaged tissue. The inflammatory factors are produced by M1 microglia through the JAK/STAT pathway, eventually leading to cell apoptosis. Meanwhile, the JAK2/STAT3 signaling pathway and the expression of lipocalin-2 and caspase-3 could increase. In the pathway, phosphorylated JAK2 and phosphorylated STAT3 function of 2 ways. They not only promote the proliferation of neurons, but also affect the differentiation direction of neural stem cells by further acting on the Notch signaling pathway. Recently, traditional Chinese medicine (TCM) is a key player in CIRI, through JAK2, STAT3, STAT1 and their phosphorylation. Therefore, the review focuses on the JAK/STAT signaling pathway and its relationship with CIRI as well as the influence of the TCM on this pathway. It is aimed at providing the basis for future clinical research on the molecular mechanism of TCM in the treatment of CIRI.

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Buyang Huanwu Decoction restores the balance of mitochondrial dynamics after cerebral ischemia-reperfusion through calcium overload reduction by the PKCε-Nampt-Sirt5 axis

Liu,

Yin,

et al. 2025

Journal of Ethnopharmacology

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To explore the regulatory effect of Buyang Huanwu Decoction on cerebral infarction based on quantitative proteomics

Cited by 5 publications

References 30 publications

Molecular mechanisms underlying the therapeutic effects of Linggui Zhugan decoction in stroke: Insights from network pharmacology and single-cell transcriptomics analysis

Molecular mechanisms underlying the therapeutic effects of Linggui Zhugan decoction in stroke: Insights from network pharmacology and single-cell transcriptomics analysis

The role of JAK/STAT signaling pathway in cerebral ischemia-reperfusion injury and the therapeutic effect of traditional Chinese medicine: A narrative review

Buyang Huanwu Decoction restores the balance of mitochondrial dynamics after cerebral ischemia-reperfusion through calcium overload reduction by the PKCε-Nampt-Sirt5 axis

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