To study the effect of oxygen carrying capacity on expressed changes of erythrocyte membrane protein in different storage times

Abstract

Erythrocyte membrane is crucial to maintain the stability of erythrocyte structure. The membrane protein on the surface of erythrocyte membrane enables erythrocyte to have plasticity and pass through the microcirculation without being blocked or destroyed. Decreased deformability of erythrocyte membrane protein will lead to a series of pathological and physiological changes such as tissue and organ ischemia and hypoxia. Therefore, this research collected 30 cases of healthy blood donors, and explored erythrocyte stored at different times relating indicators including effective oxygen uptake (Q), P50, 2,3-DPG, Na+-k+-ATP. Erythrocyte morphology was observed by electron microscopy. Western blot and immunofluorescence assay were used to detect membrane protein EPB41, S1P, GLTP, SPPL2A expression changes of erythrocyte. To explore the effective carry oxygen capacity of erythrocyte at different storage time resulting in the expression change of erythrocyte surface membrane protein.

Metabolite and protein shifts in mature erythrocyte under hypoxia

“…The insertion of cholesterol can regulate the fluidity of the human erythrocyte membrane. 12 In addition to lipids, proteins also play essential roles in maintaining the function and structure of erythrocytes. Erythrocyte membrane proteins are closely related to membrane deformability, the transport and release of oxygen molecules, the regulation of ion balance, and signal recognition and transduction.…”

Diseased Erythrocyte Enrichment Based on I-Shaped Pillar DLD Arrays

“…Erythrocytes have a unique biconcave pie-shaped structure with a highly elastic cell membrane about 5 nanometers thick, consisting of a phospholipid bilayer with a protein backbone. The cytoplasm inside can be regarded as an incompressible viscous fluid [28][29][30]. In modeling erythrocytes, we use parametric curves to draw the shape of the erythrocyte cross section.…”

Genetic architecture of non‐syndromic skeletal class III malocclusion

“…EPB41 plays a key role in cell formation and mechanical stability (Wang et al, 2020). Genetic polymorphisms in the EPB41 conferred susceptibility to MP in 158 cases and 147 controls.…”