To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

Dash, Devi Jyoti

doi:10.19080/ijoprs.2019.04.555632

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…13 R.K.Panda et al 65 cases with suspected pulmonary tuberculosis with negative results on sputum AFB smears on at least 2 samples, of negative sputum CBNAAT, were subjected to fibreoptic bronchoscopy and BAL was sent for CBNAAT, 29 came out positive with a yield of 45%. 14 Dr C.R. Chaudhary et al conducted a study on 140 patients suspected of PTB on clinic radiological basis, who were either negative for sputum smear AFB or who did not produce sputum, were subjected to FOB and BAL sent for CBNAAT, 83 came out to be positive with a yield of 59%.…”

Section: Discussionmentioning

confidence: 99%

Role Of Sputum And Bronchoalveolar Lavage CBNAAT In The Diagnosis Of PTB In Smear Negative Patients

Sivaji¹,

Singh²,

Mahendran³

2023

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AIM. To study the use of sputum and broncho alveolar lavage (BAL) for CBNAAT, as an adjunct in the diagnosis of pulmonary tuberculosis, in sputum AFB smear negative patients. OBJECTIVE. To calculate and compare the yield of sputum Cartridge Based Nucleic Acid Amplification Test (CBNAAT) and BAL CBNAAT in sputum AFB smear negative patients. MATERIALS and METHODS. 50 Patients with respiratory symptoms which are suspected of pulmonary tuberculosis with Chest X-ray showing changes consistent with active pulmonary TB and Sputum negative for AFB on two samples were subsequently subjected to sputum CBNAAT, in which 23 out of 50, came out to be positive (46% yield). The remaining 27 patients were subjected to bronchoscopy and the BAL was sent for CBNAAT, in which 23 patients came out as positive (Yield 85%). CONCLUSION. Sputum CBNAAT and BAL CBNAAT prove to be useful adjuncts in the diagnosis of presumptive PTB patients who are sputum AFB smear negative. BAL CBNAAT has higher yield than sputum CBNAAT.

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Section: Discussionmentioning

confidence: 99%

Role Of Sputum And Bronchoalveolar Lavage CBNAAT In The Diagnosis Of PTB In Smear Negative Patients

Sivaji¹,

Singh²,

Mahendran³

2023

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Evaluation of Diagnostic Methods and Rifampicin Resistance in Pulmonary Tuberculosis: A Hospital-Based Study

Joshi,

Singh,

Patidar

et al. 2024

Cureus

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Background Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis, predominantly affecting the lungs (pulmonary TB) and is a significant public health challenge in India. The study aims to analyze demographic, radiological, and clinical subgroups of pulmonary TB cases, examine the relationship between smear acid-fast bacillus (AFB examination) and cartridge-based nucleic acid amplification test (CBNAAT), evaluate CBNAAT sensitivity for Mycobacterium tuberculosis (MTB) in new and previously treated patients, and determine the proportion of rifampicin resistance. Methods This hospital-based prospective study was conducted among patients diagnosed with pulmonary TB at the Respiratory Medicine Department of a Government Hospital over 16 months (August 2019 to December 2020). The study included 150 diagnosed TB cases (new and previously treated). Data collection encompassed demographic details, clinical symptoms, comorbidities, radiological findings (chest X-ray), and microbiological results (smear AFB examination, CBNAAT). Sputum samples were subjected to Ziehl-Neelsen staining and CBNAAT for MTB detection and rifampicin resistance testing. Statistical analysis was performed using IBM SPSS Statistics version 21.0 (IBM Corp., Armonk, NY, USA). Results Of the 150 patients, 69.3% were male, and 48% were aged 21-40 years. The majority had a BMI of 18.5-24.9 kg/m² (50%) and resided in urban areas (63.3%). Common symptoms included cough (95.3%), fever (80%), and weight loss (74%). Cavitary lesions on chest X-ray were observed in 84% of patients. Smear microscopy detected MTB in 72.7% of cases, while CBNAAT detected MTB in 94% of cases. CBNAAT sensitivity for smear-positive and smear-negative samples was 93.97% and 94.12%, respectively. Rifampicin resistance was found in 3% of new cases and 6% of previously treated cases. The sensitivity of smear microscopy was 77.33%, and the sensitivity of CBNAAT was 94%. Conclusion The study underscores the high burden of pulmonary TB and the utility of CBNAAT in detecting MTB and rifampicin resistance, particularly in smear-negative samples. The findings highlight the necessity of universal drug susceptibility testing (DST) for effective TB management and the importance of addressing drug resistance to improve treatment outcomes.

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To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

Cited by 2 publications

References 8 publications

Role Of Sputum And Bronchoalveolar Lavage CBNAAT In The Diagnosis Of PTB In Smear Negative Patients

Role Of Sputum And Bronchoalveolar Lavage CBNAAT In The Diagnosis Of PTB In Smear Negative Patients

Evaluation of Diagnostic Methods and Rifampicin Resistance in Pulmonary Tuberculosis: A Hospital-Based Study

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