Tobacco as green bioreactor for therapeutic protein production: latest breakthroughs and optimization strategies

Naeem, Muhammad; Han, Rong; Ahmad, Naveed; Zhao, Weihua; Zhao, Lingxia

doi:10.1007/s10725-023-01106-w

Cited by 4 publications

(1 citation statement)

References 160 publications

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“…These characteristics contribute to achieving high levels of desired proteins, thereby simplifying the process of protein extraction and purification. Consequently, tobacco is regarded as an excellent candidate for plant-based protein production [54]. In the future, tobacco leaves may be utilized as a bioreactor under optimal conditions to enhance the accumulation of 2AP by editing additional genes involved in the 2AP biosynthetic pathway.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Targeted Mutagenesis of Betaine Aldehyde Dehydrogenase 2 (BADH2) in Tobacco Affects 2-Acetyl-1-pyrroline

Chen,

Shen,

et al. 2024

Agronomy

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2-acetyl-1-pyrroline (2AP) is a highly effective volatile compound that gives fragrance to numerous plant species and food. Mutation(s) in the betaine aldehyde dehydrogenase 2 (BADH2) gene results in the accumulation of 2AP. However, the function of BADH genes in tobacco (Nicotiana tabacum L.) remains poorly understood. In this study, we successfully obtained four betaine aldehyde dehydrogenase (BADH) genes from tobacco. Phylogenetic analysis of the protein sequences showed that two of the four BADH genes were closely related to the wolfberry (Lycium barbarum) BADH gene (LbBADH1), so we named them NtBADH1a and NtBADH1b, respectively. The other two BADH genes were orthologues of the tomato (Solanum lycopersicum) aminoaldehyde dehydrogenase 2 (SlAMADH2) gene, and were named NtBADH2a and NtBADH2b, respectively. Expression analysis revealed that the biological functions of NtBADH1a and NtBADH1b were different from those of genes NtBADH2a and NtBADH2b. We introduced mutations into NtBADH1a, NtBADH1b, NtBADH2a and NtBADH2b in tobacco using the CRISPR/Cas9 system and identified transgenic Ntbadh mutant tobacco lines. Single mutants (Ntbadh1a, Ntbadh1b, Ntbadh2a and Ntbadh2b) and double mutants (Ntbadh1a-Ntbadh1b and Ntbadh2a-Ntbadh2b) harbored deletion or insertion of nucleotides, both of which led to the production of a frameshift, preventing protein accumulation. A popcorn-like scent was noticeable in tobacco leaves from the Ntbadh2a-Ntbadh2b double mutant, but not from any single mutant or the Ntbadh1a-Ntbadh1b double mutant or the wild type. Consistent with this observation, we only detected 2AP in fresh leaves from the Ntbadh2a-Ntbadh2b double mutant. These findings indicate that only the combined inactivation of NtBADH2a and NtBADH2b results in 2AP accumulation in tobacco, which was not related to NtBADH1.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Targeted Mutagenesis of Betaine Aldehyde Dehydrogenase 2 (BADH2) in Tobacco Affects 2-Acetyl-1-pyrroline

Chen,

Shen,

et al. 2024

Agronomy

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Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

Trujillo,

Angulo

2024

Biotech & Bioengineering

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Enteric diseases by pathogenic organisms are one of the leading causes of death worldwide, particularly in low‐income countries. Despite antibiotics, access to clean water and vaccination are the most economically affordable options to prevent those infections and their health consequences. Vaccines, such as those approved for rotavirus and cholera, have played a key role in preventing several enteric diseases. However, vaccines for other pathogens are still in clinical trials. Distribution and cost remain significant barriers to vaccine access in developing regions due to poor healthcare infrastructure, cold‐chain requirements, and high production costs. Plant‐made vaccines offer a promising alternative to address these challenges. Plants can be easily grown, lowering production costs, and can be administered in oral forms, potentially eliminating cold‐chain dependency. Although there are some promising prototypes of vaccines produced in plants, challenges remain, including yields and achieving sufficient immunogenicity. This review aims to describe common enteric pathogens and available vaccines, followed by a strategic summary of plant‐made vaccine development and a discussion of plant‐made enteric vaccine prototypes. Trends to overcome the key challenges for plant‐made vaccines are identified and placed in perspective for the development of affordable and effective vaccines for populations at the highest risk of enteric diseases.

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Emerging Compost Extract for Heavy Metal Bioremediation and Preserving the Antioxidant Activity of Celery Cabbage: A Sustainable Approach

Haroun,

Barham,

Wang

et al. 2024

Biocatalysis and Agricultural Biotechnology

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Tobacco as green bioreactor for therapeutic protein production: latest breakthroughs and optimization strategies

Cited by 4 publications

References 160 publications

CRISPR/Cas9-Mediated Targeted Mutagenesis of Betaine Aldehyde Dehydrogenase 2 (BADH2) in Tobacco Affects 2-Acetyl-1-pyrroline

CRISPR/Cas9-Mediated Targeted Mutagenesis of Betaine Aldehyde Dehydrogenase 2 (BADH2) in Tobacco Affects 2-Acetyl-1-pyrroline

Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

Emerging Compost Extract for Heavy Metal Bioremediation and Preserving the Antioxidant Activity of Celery Cabbage: A Sustainable Approach

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