Tobacco Peroxidase as a New Reagent for Amperometric Biosensors

Selective Growth of Co-electrodeposited Mn2O3-Au Spherical Composite Network Towards Enhanced Non-enzymatic Hydrogen Peroxide Sensing

“…A comparison of Mn 2 O 3 -Au sensing performance with clean Au surface [40], nanoporous Au [46], and Au nanoparticles [41] reveal a lower detection limit of our system. Further, comparison with HRP based enzymatic H 2 O 2 sensors in similar working range of concentration reveal that the sensing performance of our Mn 2 O 3 -Au system is comparable [47], in some cases better [42] than enzyme based H 2 O 2 sensors. Hence, characteristics such as easy synthesis, good sensitivity, low detection limit and negligible interference from other analytes, suggest that the Mn 2 O 3 -Au composite would be a good candidate for non-enzymatic H 2 O 2 sensing.…”

Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission

“…The use of this reaction in this study was based on two main issues. Firstly, as was mentioned before, the higher fungi contain an extended set of peroxidases, includ ing the secreted enzymes of this group [20][21][22][23][24][25][26]. Sec ond, it was shown experimentally that the ROS (H 2 O 2 in particular) generated by the fungus during its meta bolic development could participate in mechanisms of the fungal bioluminescence [11][12][13][14].…”

“…The majority of them are heme containing enzymes. Heme containing per oxidases from basidiomycetes have been studied in sufficient detail [19][20][21][22][23][24][25][26]. They play an important role in fungi metabolism and are of interest for biotechno logical applications.…”

Interprotein Coupling Enhances the Electrocatalytic Efficiency of Tobacco Peroxidase Immobilized at a Graphite Electrode

“…6 This mechanism is expected to apply also for anionic tobacco peroxidase (TOP), which was early recognized as a promising building block for biosensors due to its high stability and substrate specificity. [7][8][9] A variety of strategies for immobilization of tobacco peroxidase have been reported, including physisorption on solid substrates, such as bare gold 10 and graphite [11][12][13][14] and on selfassembled thiol monolayers 15 or, alternatively, it has been embedded into a redox-active polymer gel. 16 In this work we show how the simultaneous incubation and deposition of a mixture of TOP and the crossing coupling agents 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS), which is likely to produce an extensive intra-and inter-protein coupling, results in a significant enhancement of the electrocatalytic hydrogen peroxide reduction and an overall improvement of the biosensor performance.…”