Tobacco Stalk as Source of CMCase Enzyme Production of Actinomycetes Isolated from Rhizosphere of Tobacco (Nicotiana tabacum L.) by Submerged Fermentation

Abstract

Tobacco stalk is tobacco plant waste which contains about 56% cellulose so that it is possible to use it as a substrate for the production of cellulase, namely CMCase. The study conducted in several stages, namely screening the cellulolytic activity of 71 actinomycetes isolates, optimizing CMCase production by three actinomycetes with the best CMCase activity index using tobacco stalk as substrate through submerged fermentation, and morphology identification actinomycetes with the highest CMCase activity. A total of 71 actinomycetes have been isolated from the rhizosphere of tobacco plant. In this study, the ability of actinomycetes to produce cellulase through submerged fermentation using tobacco stalk substrate powder. There were about 71 actinomycetes isolated and all of them have cellulolytic activities. Based on the screening of cellulolytic activity on CMC (carboxymethyl cellulose) agar plate, the enzyme activity index of ATG 38 isolate was higher than others, with 2.50 ± 0.26 respectively. The cellulase production using tobacco stalk as substrate through submerged fermentation was optimal showing that CMCase production of actinomycete ATG 38 isolates maximal after four days incubation with 0,40 mU/mL enzyme activity. Microscopic morphological observations showed that actinomycete ATG 38 isolate belongs to the genus Streptomyces.

Tobacco as bioenergy and medical plant for biofuels and bioproduction

“…Utarti et al studied 71 strains of actinomycetes using tobacco as a substrate, optimizing their cellulase production through SSF of tobacco stalks. The highest cellulase activity reached 0.40 mU/mL in actinomycete cultures, and further microscopic observations confirmed their classification as belonging to the genus Streptomyces [ 156 ]. There are also studies utilizing tobacco for the production of galactosidase.…”