Tobacco Tsip1, a DnaJ-Type Zn Finger Protein, Is Recruited to and Potentiates Tsi1-Mediated Transcriptional Activation

Ham, Byung Kook; Park, Jeong Mee; Lee, Suk-bae; Kim, Min Jung; Lee, In Ju; Kim, Ki-Jeong; Kwon, Chang Seob; Paek, Kyung Hee

doi:10.1105/tpc.106.043158

Cited by 58 publications

(69 citation statements)

References 51 publications

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“…Isolation of NtTfd1 as a Tsip1-interacting protein by yeast two-hybrid screening Tsip1 was identified as a transcriptional co-activator by interaction with EREBP/AP2-type transcription factor Tsi1 (Ham et al, 2006;Park et al, 2001). To carry out further functional study of Tsip1, we performed yeast two-hybrid screening of tobacco cDNA library using Tsip1 as bait.…”

Section: Resultsmentioning

confidence: 99%

“…Tsip1 is a transcriptional co-activator of an EREBP/AP2-type transcription factor, Tsi1 (for Tobacco stress-induced gene 1), and relocalizes with Tsi1 to the nucleus from the chloroplast upon salicylic acid treatment (Ham et al, 2006;Park et al, 2001). The protein relocalization in response to biotic and abiotic stress signals are relatively common in plants (Caplan et al, 2008;Hwang and Sheen, 2001;Igarashi et al, 2001;Yeung et al, 2008) and could have evolved as a defense mechanism of plants to pathogens and environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

“…This could be an inefficient process because plants must expend much energy and time. Tsip1 gene expression rapidly increases upon treatment with salicylic acid, ethephon, and salt, and upon exposure to pathogens (Ham et al, 2006). Dynamic gene expression patterns of Tsip1 suggest that Tsip1 alone or along with other binding-partner proteins can functionally respond to various extracellular signals.…”

Section: Introductionmentioning

confidence: 99%

“…Tsip1 contains a Zn-finger motif similar to the bacterial DnaJtype protein and has been classified as a new DnaJ-type Zn finger protein (Ham et al, 2006). However, Tsip1 does not contain the DnaJ domain per se (Qiu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Nicotiana tabacum Tsip1-Interacting Ferredoxin 1 Affects Biotic and Abiotic Stress Resistance

Huh

Lee

Ham³

et al. 2012

Molecules and Cells

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*Tsip1, a Zn finger protein that was isolated as a direct interactor with tobacco stress-induced 1 (Tsi1), plays an important role in both biotic and abiotic stress signaling. To further understand Tsip1 function, we searched for more Tsip1-interacting proteins by yeast two-hybrid screening using a tobacco cDNA library. Screening identified a new Tsip1-interacting protein, Nicotiana tabacum Tsip1-interacting ferredoxin 1 (NtTfd1), and binding specificity was confirmed both in vitro and in vivo. The four repeats of a cysteine-rich motif (CXXCXGXG) of Tsip1 proved important for binding to NtTfd1. Virus-induced gene silencing of NtTfd1, Tsip1, and NtTfd1/Tsip1 rendered plants more susceptible to salinity stress compared with TRV2 control plants. NtTfd1-and Tsip1-silenced tobacco plants were more susceptible to infection by Cucumber mosaic virus compared with control plants. These results suggest that NtTfd1 might be involved in the regulation of biotic and abiotic stresses in chloroplasts by interaction with Tsip1.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nicotiana tabacum Tsip1-Interacting Ferredoxin 1 Affects Biotic and Abiotic Stress Resistance

Huh

Lee

Ham³

et al. 2012

Molecules and Cells

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“…Arabidopsis wildtype and transgenic plants had the ecotype Columbia-0 background and were grown in a 16 h light/8 h dark photoperiod at 23°C in soil. For the constitutive expression of APUM5 (At3g20250), a modified pCAMBIA2300 vector was used (25), and the APUM5 ORF was amplified using Pfu DNA polymerase (Promega) and cloned. For the repression of APUM5 expression, the RNAi technique was exploited using the pHANNIVAL vector system, which can transcribe self-complementary hairpin RNA molecules (26).…”

Section: Methodsmentioning

confidence: 99%

ArabidopsisPumilio protein APUM5 suppressesCucumber mosaic virusinfection via direct binding of viral RNAs

Huh¹,

Kim

Paek

2012

Proc. Natl. Acad. Sci. U.S.A.

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Posttranscriptional/translational regulation of gene expression is mediated by diverse RNA binding proteins and plays an important role in development and defense processes. Among the RNAbinding proteins, the mammalian Pumilio RNA-binding family (Puf) acts as posttranscriptional and translational repressors. An Arabidopsis Puf mutant, apum5-D, was isolated during a T-DNA insertional mutant screen for mutants with reduced susceptibility to Cucumber mosaic virus (CMV) infection. Interestingly, CMV RNA contained putative Pumilio-homology domain binding motifs in its 3′ untranslated region (UTR) and internal places in its genome. APUM5 directly bound to the 3′ UTR motifs and some internal binding motifs in CMV RNAs in vitro and in vivo. We showed that APUM5 acts as a translational repressor that regulates the 3′ UTR of CMV and affects CMV replication. This study uncovered a unique defense system that Arabidopsis APUM5 specifically regulates CMV infection by the direct binding of CMV RNAs. plant defense | Pumilio RNA binding protein | TuMV P lant viruses are the obligate pathogens, and host proteins facilitate the multiplication of viruses by affecting processes such as viral replication, cell-to-cell movement, and systemic movement of the virus (1). These processes occur upon the interaction between the virus and host proteins or after the suppression of the host basal defense mechanism and often lead to abnormal phenotypes of virus-infected host plants, such as small, highly branched bushes with deformed leaves, stunting, and reduced apical dominance (2-4). Thus, the reduced growth and developmental changes in the virus-infected plants are typical symptoms of virus infection and signify that the virus has undergone a successful life cycle. To understand the molecular mechanisms underlying viral multiplication and symptoms in plants, it is necessary to identify and characterize the host factors involved in these processes.To identify novel host factors involved in the multiplication of plant RNA viruses in susceptible plants, T-DNA insertion mutants of Arabidopsis thaliana Col-0 ecotype, in which CMVKor multiplication is abrogated (5), were screened. An Arabidopsis Puf mutant, apum5-D, in which Cucumber mosaic virus (CMV) multiplication was affected during viral spreading, was isolated. APUM5 contains the Pumilio-homology domain (PHD) and encodes a putative Puf, which was originally identified in Drosophila melanogaster and Caenorhabditis elegans (6). Pufs are highly conserved in various organisms and work as posttranscriptional and translational repressors (7,8). PHD has RNA binding activity; there are eight repeats with three alpha helices in each repeat. The inner surface of the PHD binds the RNA. The outer surface of the domain permits protein-protein interactions with diverse proteins, such as deadenylase and general translation factors (9, 10). Arabidopsis Pufs also exhibit RNA-binding activity and have conserved binding motifs (11,12). However, plant Puf functions have not yet been fully identified or charact...

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Stress Physiology of Higher Plants: Cross‐Talk between Abiotic and Biotic Stress Signaling

Fujita¹,

Fujita

Takahashi³

et al. 2009

Plant Stress Biology

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Plants have developed a number of unique mechanisms to cope with diverse biotic and abiotic stresses during the course of evolution. Under natural circumstances, plants may be exposed to multiple environmental stresses simultaneously. However, the molecular mechanism involved in dealing with each stressor has been studied relatively independently; thus, to produce a comprehensive picture of the plant response to multiple environmental stresses under natural conditions it is necessary to identify the convergence points between these biotic and abiotic stress signaling pathways. Several key players involved in the cross-talk between stress signaling pathways have been identified, including transcription factors and kinases. Accumulating evidence also suggests that the pathways regulated by abscisic acid, salicylic acid, jasmonic acid, and ethylene, as well as reactive oxygen species, play crucial roles in the cross-talk between biotic and abiotic stress signaling. Moreover, recent observations suggest that epidermal tissues, including the cuticle and stomata, are pivotal points of convergence between multiple stress signaling pathways and function as a first line of defense against environmental stress. IntroductionUnlike animals, plants are immobile, which means they cannot select their own environment; thus, they have evolved a wide range of unique and sophisticated survival strategies to cope with biotic and abiotic stresses. Significant effort has been focused on understanding the signal transduction pathways activated by these stimuli and our knowledge in this area is considerable. In several cases, we Plant Stress Biology. Edited by H. Hirt

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Tobacco Tsip1, a DnaJ-Type Zn Finger Protein, Is Recruited to and Potentiates Tsi1-Mediated Transcriptional Activation

Cited by 58 publications

References 51 publications

Nicotiana tabacum Tsip1-Interacting Ferredoxin 1 Affects Biotic and Abiotic Stress Resistance

Nicotiana tabacum Tsip1-Interacting Ferredoxin 1 Affects Biotic and Abiotic Stress Resistance

ArabidopsisPumilio protein APUM5 suppressesCucumber mosaic virusinfection via direct binding of viral RNAs

Stress Physiology of Higher Plants: Cross‐Talk between Abiotic and Biotic Stress Signaling

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