Tolerance of Saccharomyces cerevisiae K35 to lignocellulose-derived inhibitory compounds

Lee, Hawon; Cho, Dae Haeng; Kim, Yong Hwan; Shin, Soo‐Jeong; Kim, Sung Bong; Han, Sung Ok; Lee, Jinwon; Kim, Seung Wook; Park, Chulhwan

doi:10.1007/s12257-010-0474-4

Cited by 41 publications

(24 citation statements)

References 20 publications

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“…This means that the recovered glucose was fermentable but an additional process was needed to remove the inhibitors needed before fermentation. Lee et al have reported the effects of the inhibitory compounds, particularly on the ethanol production [22,23]. …”

Section: Resultsmentioning

confidence: 99%

“…The reactions were performed at 50°C in 0.05 M of citrate buffer (pH 4.8) at 150 rpm. Fermentation with glucose, produced by saccharification, was performed using Saccharomyces cerevisiae K35 [22,29]. The cells were inoculated with the YM broth medium and incubated at 30°C and 200 rpm for 24 h. For the main culture, 0.5 mL of inoculums in the main medium was prepared in 250 mL Erlenmeyer flasks.…”

Section: Methodsmentioning

confidence: 99%

“…The intensities of the amorphous (2 θ =18°) and crystal (2 θ = 22°) regions, as reported by Segal et al . [22], were used to calculate CrI as follows:…”

Section: Methodsmentioning

confidence: 99%

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Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia

Kim

Lee

et al. 2013

Biotechnol Biofuels

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108

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BackgroundUse of lignocellulosic biomass has received attention lately because it can be converted into various versatile chemical compounds by biological processes. In this study, a two-step pretreatment with dilute sulfuric acid and aqueous ammonia was performed efficiently on rice straw to obtain fermentable sugar. The soaking in aqueous ammonia process was also optimized by a statistical method.ResultsResponse surface methodology was employed. The determination coefficient (R2) value was found to be 0.9607 and the coefficient of variance was 6.77. The optimal pretreatment conditions were a temperature of 42.75°C, an aqueous ammonia concentration of 20.93%, and a reaction time of 48 h. The optimal enzyme concentration for saccharification was 30 filter paper units. The crystallinity index was approximately 60.23% and the Fourier transform infrared results showed the distinct peaks of glucan. Ethanol production using Saccharomyces cerevisiae K35 was performed to verify whether the glucose saccharified from rice straw was fermentable.ConclusionsThe combined pretreatment using dilute sulfuric acid and aqueous ammonia on rice straw efficiently yielded fermentable sugar and achieved almost the same crystallinity index as that of α-cellulose.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia

Kim

Lee

et al. 2013

Biotechnol Biofuels

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108

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“…According to the previous description, the concentrations of glucose, xylose, vanillin and syringaldehyde in the hydrolysate and fermentation broth were analyzed by HPLC with a refractive index detector using an Aminex HPX-87H column (300 mm 9 78 mm) [21]. The concentrations of furfural and HMF were detected with a C-18 column (250 mm 9 4.6 mm) and a UV detector set at 210 nm [22]. The concentrations of the total phenolic compounds (TPC) were estimated colorimetrically by the Folin-Ciocalteu method [23].…”

Section: Methodsmentioning

confidence: 99%

Effect of lignocellulose-derived inhibitors on the growth and d-lactic acid production of Sporolactobacillus inulinus YBS1-5

Bai

Gao

et al. 2015

Bioprocess Biosyst Eng

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The impact of lignocellulose-derived inhibitors on the cell growth and D-lactic production of Sporolactobacillus inulinus YBS1-5 was investigated. At high concentrations, both furans and phenolics, such as furfural, HMF, syringaldehyde and vanillin, affected cell growth and D-lactic acid production and syringaldehyde exhibited the highest. Further experiments showed that only vanillin caused cellular membrane damage. Based on the Biolog approach, in vivo studies on intact S. inulinus cells indicated that phenolics had a stronger inhibitory effect than furan derivatives on the metabolic activity of the concerned substrates related with the key enzymes of D-lactic acid fermentation. The direct in vitro inhibitory effect of the model compounds on the four key enzymes displayed similar patterns. Syringaldehyde was the strongest inhibitor. In general, comparison with published results for other microorganisms indicated that strain YBS1-5 was a robust microorganism against inhibitors of lignocellulose hydrolysate. Notably, in concentrated corn stover hydrolysate, S. inulinus YBS1-5 produced 70.7 g/L D-lactic acid, which was 87.7 % of the yield from the control experiment. However, the fermentation time was prolonged 36 h. In order to improve fermentation rate, a detoxification technology or more robust mutant to phenolics especially syringaldehyde should be developed.

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“…The generation of fermentation inhibitors like acetic acid, hydroxy methyl furfural, furfural, glycol aldehyde and soluble phenolic compounds is also a prominent issue to be solved (Cavka et al, 2011;Sanda et al, 2011;Carter et al, 2011a, b;Raghu et al, 2011;Yong-Jin et al, 2011;Lee et al, 2011;Huang et al, 2011;Zhang et al, 2011;Bellido et al, 2011;Geddes et al, 2011;Sainio et al, 2011;Jayakody et al, 2011). A variety of biomass (cashew apple pulp, coffee pulp waste, switch grass, oil palm empty fruit bunches, seaweeds, bamboo, sugar-cane tops) are being examined as economically viable feedstock for the production of bioethanol (Sindhu et al, 2011;Zhao-Yong et al, 2011;Shenoy et al, 2011;Martin and Grossmann, 2011;Han et al, 2011;Yanagisawa et al, 2011aYanagisawa et al, , 2011b.…”

Section: Introductionmentioning

confidence: 99%

Assessment of holocellulose for the production of bioethanol by conserving Pinus radiata cones as renewable feedstock

Victor

Pulidindi

Gedanken

2015

Journal of Environmental Management

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Tolerance of Saccharomyces cerevisiae K35 to lignocellulose-derived inhibitory compounds

Cited by 41 publications

References 20 publications

Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia

Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia

Effect of lignocellulose-derived inhibitors on the growth and d-lactic acid production of Sporolactobacillus inulinus YBS1-5

Assessment of holocellulose for the production of bioethanol by conserving Pinus radiata cones as renewable feedstock

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