SUMMARYThe environmental conditions and test duration in rainfall simulators have been major constraints to find reliable differences in preharvest sprouting (PHS) among wheat cultivars. This study aimed to elucidate the temperature conditions and degree-days (DD) that enable higher discrimination of genotypes in a PHS test. Thirteen genotypes with different degrees of PHS (BR 18, BRS 220, CD 104, CD 105, CD 108, CD 114, CD 116, Frontana, IPR 085, IPR 128, IPR 136, OR1 and Safira) were evaluated in a rainfall simulator, during four years, conducting two trials per year. The tests were carried on 20 spikes per cultivar, kept in a vertical position on Styrofoam plates, spaced 10 cm between rows and 5 cm within the row. The air temperature data were recorded on a thermograph and the DD were calculated from the mean daily air temperature assuming a base temperature of 4 °C. The Falling Number (FN) parameter and germination percentage (GP) data were averaged for each test. The temperatures (minimum, mean and maximum) and the DD were regressed and correlated with FN and GP. Any increase in the minimum and mean air temperature and accumulated DD decreased FN values and increased GP. Higher genotypic discrimination was achieved at an average air temperature of 21.5 °C and 35 DD. These values can be used as reference to set simulated rain testing condition for screening genotype for PHS based on FN or pericarp rupture of grains.