Toll-Like Receptor 2 and NALP2 Mediate Induction of Human Beta-Defensins by<i>Fusobacterium nucleatum</i>in Gingival Epithelial Cells

Ji, Sang-Hoon; Shin, Ji Eun; Kim, Young Sook; Oh, Jihyun; Min, Byung‐Moo; Choi, Youngnim

doi:10.1128/iai.00449-08

Cited by 62 publications

(76 citation statements)

References 36 publications

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“…However, fewer studies have addressed the contribution of TLRs to BD expression induced by bacterial infections. TLR2 mediates hBD2 expression induced by F. nucleatum and S. aureus, whereas TLR2 and TLR4 are required for the induction of hBD2 by Propionibacterium acnes (34). Here we showed that 52145-⌬wca K2 activated the expression of hBD2 via TLR2 and TLR4.…”

Section: Discussionmentioning

confidence: 56%

Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

et al. 2010

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Section: Discussionmentioning

confidence: 56%

Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

et al. 2010

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“…A flow cytometric invasion assay was performed as described previously. 39 HOK-16B cells were infected with 5-(and 6-) carboxy-fluorescein diacetate succinimidyl ester (CFSE; Molecular Probe)-labeled bacteria at a multiplicity of infection (MOI) of 1000 in KGM without antibiotics for 4 h. Infected HOK-16B cells were washed with PBS and detached with trypsin-EDTA. After quenching the fluorescence of the bacteria bound on the surface with 0.4% trypan blue, the cells were analyzed using a FACSCalibur (BD Bioscience).…”

Section: Isolation and Identification Of P Gingivalis From Clinical mentioning

confidence: 99%

Association of the invasion ability ofPorphyromonas gingivaliswith the severity of periodontitis

Baek

Ji²,

Kim

et al. 2015

Virulence

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(2015) Association of the invasion ability of Porphyromonasgingivalis with the severity of periodontitis, Virulence, 6:3, 274-281, DOI: 10.1080/21505594.2014 Keywords: cytokine proteolytic activity, invasion ability, Porphyromonas gingivalis, periodontitis, severityPorphyromonas gingivalis is one of the well-characterized periodontal pathogens involved in periodontitis. The invasive and proteolytic activities of P. gingivalis clinical isolates have been shown to be associated with heterogenic virulence, as determined in a mouse abscess model. The aims of the present study were to identify a P. gingivalis strain with a low virulence among clinical isolates, based on its invasive ability and cytokine proteolytic activities, and to explore the preferential degradation of a certain cytokine by P. gingivalis. P. gingivalis ATCC 33277, W50, and 10 clinical isolates were used. After incubating bacteria with IL-4, IL-6, IL-10, IL-17A, TNFa, IFNg, and IL-1a, the amounts of remaining cytokines were determined by ELISA. Invasion ability was measured by a flow cytometric invasion assay. There was inter-strain variability both in the cytokine proteolytic activities and invasion ability. In addition, differential degradation of cytokines by P. gingivalis was observed: while IFNg and IL-17A were almost completely degraded, inflammatory cytokines TNFa and IL-1a were less susceptible to degradation. Interestingly, the invasion index, but not cytokine proteolytic activities, of P. gingivalis had strong positive correlations with clinical parameters of subjects who harbored the isolates. Therefore, the invasive ability of P. gingivalis is an important virulence factor, and the bacterial invasion step may be a good target for new therapeutics of periodontitis.

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“…In vitro, F. nucleatum can mediate coaggregation (coadherence among planktonic bacteria) between many species of dental colonizers (19,20) and therefore has been proposed to function as a bridging organism that stabilizes the developing dental plaque (21)(22)(23). Apart from its ability to bind multiple bacterial species, F. nucleatum is capable of adhering to (24) and invading (25)(26)(27)(28)(29) various mammalian cell types, inducing the secretion of proinflammatory cytokines that contribute to the initiation and progression of periodontal diseases (29)(30)(31). F. nucleatum was also shown to shuttle other noninvasive bacteria into epithelial cells (32).…”

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confidence: 99%

Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

et al. 2015

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Fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. In the oral environment, F. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. Many of these interactions (called coadherences or coaggregations) are galactose sensitive. Galactose-sensitive interactions are also involved in the binding of F. nucleatum to host cells. Hemagglutination of some F. nucleatum strains is also galactose sensitive, suggesting that a single galactose-sensitive adhesin might mediate the interaction of fusobacteria with many partners and targets. In order to identify the fusobacterial galactose-sensitive adhesin, a system for transposon mutagenesis in fusobacteria was created. The mutant library was screened for hemagglutination deficiency, and three clones were isolated. All three clones were found to harbor the transposon in the gene coding for the Fap2 outer membrane autotransporter. The three fap2 mutants failed to show galactose-inhibitable coaggregation with Porphyromonas gingivalis and were defective in cell binding. A fap2 mutant also showed a 2-log reduction in murine placental colonization compared to that of the wild type. Our results suggest that Fap2 is a galactose-sensitive hemagglutinin and adhesin that is likely to play a role in the virulence of fusobacteria.

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Toll-Like Receptor 2 and NALP2 Mediate Induction of Human Beta-Defensins byFusobacterium nucleatumin Gingival Epithelial Cells

Abstract: We previously reported that infection by

Cited by 62 publications

References 36 publications

Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

Association of the invasion ability ofPorphyromonas gingivaliswith the severity of periodontitis

Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth

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