Toll-Like Receptor 2 Expression and Peripheral Blood CD4+/CD8+ T Cell Ratio in COPD

Abstract

oBJeCtIVes:We aimed to evaluate toll-like receptor 2 (TLR-2) expression on monocytes and peripheral blood CD4+/CD8+ T cell ratio, as well as the relationship of these cells with pulmonary functions in chronic obstructive pulmonary disease (COPD) patients. MAterIAl And Methods:Forty COPD patients and 40 healthy volunteers were included. Participants were analysed in four groups according to their smoking status. Peripheral blood CD4+ and CD8+ T cells and monocyte TLR-2 expression were measured by flow cytometry in the whole study population.resUlts: No significant difference was observed in TLR-2 expression, number of CD4+ and CD8+ T cells, and CD4+/CD8+ T cell ratio between the study groups. CD4+/CD8+ T cell ratio and FEV 1 /FVC were found to have a mild positive correlation (r=0.295, p=0.022). A mild negative correlation was observed between smoking intensity and CD4+/CD8+ T cell ratio (r=-0.274, p=0.034). ConClUsIon:We demonstrated a mild correlation between pulmonary functions and peripheral blood CD4+/CD8+ T cell ratio. However, we did not find a significant difference in TLR-2 expression of CD14+ monocytes in patients with airway obstruction. KeY words: COPD, inflammation, innate immunity, CD4+/ CD8+ T lymphocytes, TLRAMAÇ: Bu çalışmada kronik obstrüktif akciğer hastalığı (KOAH) bulunan hastalarda monositer Toll-Like Reseptör-2 (TLR-2) ekspresyonu ve periferik kan CD4+/CD8+ T hücre oranlarının yanı sıra bu hücrelerin solunum fonksiyonlarıyla ilişkisinin araştırılması amaçlanmıştır. GereÇ Ve YönteMler:Kırk KOAH'lı hasta ve 40 sağlıklı gönüllü çalışmaya dahil edildi. Çalışmaya alınan katılımcılar sigara içme durumlarına göre 4 grupta analiz edildi. Periferik kan CD4+/CD8+ T hücreleri ve monositer TLR-2 ekspresyonu "flow-sitometri" yön-temiyle çalışıldı.BUlGUlAr: Çalışma grupları arasında TLR-2 ekspresyonu, CD4+, CD8+ T hücreleri ve CD4+/CD8+ oranları arasında istatistiksel anlamlı fark saptanmadı. CD4+/CD8+ oranları ve FEV 1 /FVC arasında hafif düzeyde pozitif ilişki mevcuttu (r=0,295, p=0,022). Sigara içme yoğunluğu ve CD4+/CD8+ oranları ise hafif derecede ters ilişkiliydi (r=-0,274, p=0,034). sonUÇ: Solunum fonksiyonları ve periferik kan CD4+/CD8+ oranları arasında hafif düzeyli bir ilişki saptamış olsak da, hava yolu obstrüksiyonu olan hastalarda CD 14+ monositlerdeki TLR-2 ekspresyonunda bir farklılık gözleyemedik.

<p>The Role of Toll-Like Receptors 2 and 4 in the Pathogenesis of Chronic Obstructive Pulmonary Disease</p>

“…10,45 Some researchers have reported no changes in TLR4 expression on blood monocytes and neutrophils in patients with COPD compared to non-smokers and smokers with normal pulmonary function (Table 2). 10,43,45,46 In general, the results of studies presented in the literature suggest that there are differences between local and systemic effects of PAMP-mediated activation of TLR2/ TLR4 in COPD. In addition, it is known that adverse environmental factors, such as cigarette smoking and air pollution, have a great impact on these processes.…”