Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line

Moue, Masayuki; Tohno, Masanori; Shimazu, Tomoyuki; Kido, Taketomo; Aso, Hisashi; Saito, Tadao; Kitazawa, Haruki

doi:10.1016/j.bbagen.2007.11.006

Cited by 123 publications

(110 citation statements)

References 49 publications

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“…Epithelial TLR expression is thought to play a key role in the host defense against pathogens by triggering innate immune responses, through activation of NF-kB and mitogen-activated protein kinase (MAPK) pathways, that ultimately lead to the production of pro-inflammatory cytokines and chemokines (Stadnyk, 2002;Oswald, 2006). (Kaeffer et al 1993); d) porcine intestinal epitheliocyte (PIE) cells (Moue et al 2008).…”

Section: Pig Intestinal Cell Lines For In Vitro Studiesmentioning

confidence: 99%

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Roselli

Pieper

Rogel–Gaillard

et al. 2017

Animal Feed Science and Technology

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms HIGHLIGHTS-Updated literature overview about probiotics, prebiotics and synbiotics on pig gut health is provided -Both in vivo studies performed in pigs and in vitro studies conducted in pig intestinal cell lines are described -A critical outcome of the described results is provided -The concept of "proxy measurements" for pig gut health is widely discussed -The link between gut microbiota and mucsal immune system is described Abstract:Probiotics are live microorganisms that can confer a health benefit on the host, and amongst various mechanisms probiotics are believed to exert their effects by production of antimicrobial substances, competition with pathogens for adhesion sites and nutrients, enhancement of mucosal barrier integrity and immune modulation. Through these activities probiotics can support three core benefits for the host: supporting a healthy gut microbiota, a healthy digestive tract and a healthy immune system. More recently, the concept of combining probiotics and prebiotics, i.e. synbiotics, for the beneficial effect on gut health of pigs has attracted major interest, and examples of probiotic and prebiotic benefits for pigs are pathogen inhibition and immunomodulation. Yet, it remains to be defined in pigs, what exactly is a healthy gut. Because of the high level of variability in growth and feed conversion between individual pigs in commercial production systems, measuring the impact of probiotics on gut health defined by improvements in overall productivity requires large experiments. For this reason, many studies have concentrated on measuring the effects of the feed additives on proxies of gut health including many immunological measures, in more controlled experiments. With the major focus of studying the balance between gut microbiology, immunology and physiology, and the potential for prevention of intestinal disorders in pigs, we therefore performed a literature review of the immunomodulatory effects of probiotics, either alone or in combination with prebiotics, based on in vivo, in vitro and ex vivo porcine experiments. A 3 consistent number of studies showed the potential capacity in terms of immunomodulatory activities of these feed additives in pigs, but contrasting results can also be obtained from the literature.Reasons for this are not clear but could be related to differences with respect to the probiotic strain used, experimental settings, diets, initial microbiota colonization, administration route, time and frequency of administration of the probiotic strain and sampling for analysis. Hence, the use of proxy measurements of enteric health based on observable immunological parameters presents significant problems at the moment, and cannot be considered robust, reliable predictors of the probiotic activity in vivo, in relation to pig gut health. In c...

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Section: Pig Intestinal Cell Lines For In Vitro Studiesmentioning

confidence: 99%

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Roselli

Pieper

Rogel–Gaillard

et al. 2017

Animal Feed Science and Technology

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“…However, inappropriate TLR signaling can contribute to loss of tolerance and result in tissue injury (1,13); the best example of such injury is the intestinal damage mediated by the inflammatory response triggered by the interaction between lipopolysaccharide (LPS) and TLR4. LPS present in the outer membranes of some Gram-negative pathogens such as enterotoxigenic Escherichia coli (ETEC) triggers the production of proinflammatory mediators that may contribute to intestinal inflammation and damage during the infection (21,24). Thus, while TLR4 recognition of LPS is required for clearance of Gramnegative organisms, it is believed that excessive and/or prolonged proinflammatory cytokine secretion can be harmful to the host (1,13).…”

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confidence: 99%

“…Moreover, weaning is associated with upregulation of IL-1, IL-6, and tumor necrosis factor alpha (TNF-␣) in the intestine, and this early inflammatory response may contribute to both anatomical and functional intestinal disorders in piglets (28). Previously, we studied the expression of TLRs in porcine intestinal epithelial cells derived from neonatal pigs and found that, of the TLRs, TLR4 mRNA was preferentially expressed (24). Therefore, TLR4 expressed on IECs may have a role in the inflammatory response associated with weaning.…”

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confidence: 99%

“…In order to study the mechanisms by which swine IECs induce an immune response to antigens and the potential immunoregulatory effect of immunobiotics, we previously established a clonal porcine intestinal epitheliocyte cell line (PIE cells) originally derived from intestinal epithelia isolated from an unsuckled neonatal swine (24). Studies of TLR1 to TLR9 and MD-2 mRNA expressions in PIE cells demonstrate that TLR4 is the most strongly expressed of these receptors.…”

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confidence: 99%

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Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway

et al. 2012

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“…One μg of RNA was used to generate firststrand cDNA using RevertAid Premium Reverse Transcriptase (Fermentas Inc., Germany), with a blend of oligo(dT) 18 and random hexamer primers (Fermentas). Moue et al (2008) Primers for the amplification of TLR2, TLR4, TLR5, TLR9 and β-actin genes were designed according to Moue et al (2008) (Table 1). Primers for the amplification of MYD88 and NF-κB genes were designed using the gene sequences of MYD88 (NM001099923.1) and NF-κB (NM001048232.1) from the GenBank database and using Primer-Blast software.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-κB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs

Chytilová

Nemcová

Gancarčíková

et al. 2014

Acta Veterinaria Hungarica

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The present study analyses the effect of flax-seed oil rich in n-3 polyunsaturated fatty acids (PUFAs), the probiotic strain Lactobacillus plantarum -Biocenol TM LP96 and their combination on the expression level of selected Toll-like receptor (TLR) genes (TLR2, TLR4, TLR5, TLR9) and their downstream molecules (myeloid differentiation factor 88, MyD88; nuclear factor-κB, NF-κB) in the jejunum of gnotobiotic pigs challenged with enterotoxigenic Escherichia coli (ETEC). The results show that both immunomodulators are able to modulate the RNA level of at least one of the target molecules and thus regulate pathogeninduced inflammation. We confirmed that not only probiotic lactobacilli or flaxseed oil alone but also their synergistic action has great potential in the prevention and treatment of porcine colibacillosis. The results give an insight into one of the possible mechanisms by which natural agents, such as probiotic lactobacilli and flax-seed oil, exert their immunoregulatory properties during pathogen-induced inflammation.

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Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line

Cited by 123 publications

References 49 publications

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway

Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-κB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs

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