Toll-like Receptor 4 Mediates Induction of the Bcl10-NFκB-Interleukin-8 Inflammatory Pathway by Carrageenan in Human Intestinal Epithelial Cells

Bhattacharyya, Sumit; Gill, Ravinder K.; Chen, Mei Ling; Zhang, Fuming; Linhardt, Robert J.; Dudeja, Pradeep K.; Tobacman, Joanne K.

doi:10.1074/jbc.m708833200

Cited by 152 publications

(142 citation statements)

References 48 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…The reactive oxygen species inhibitor Tempol only partially inhibits the carrageenan-induced increases in NF-B and IL-8 because carrageenan-initiated inflammation proceeds through both a reactive oxygen species-mediated pathway and an innate immune-mediated signaling pathway requiring TLR4 and BCL10 (47,48). In contrast, Tempol completely blocked the increase in Wnt9A expression and the carrageenan-induced increase in Wnt signaling in NCM460 cells (3), as well as increases in total sulfated GAGs and C4S.…”

Section: Discussionmentioning

confidence: 84%

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Bhattacharyya

Feferman

Tobacman

2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background: Mechanism by which Wnt expression was increased by carrageenan exposure was unknown. Results: Sp1 activated Wnt9A transcription through changes in arylsulfatase B, chondroitin 4-sulfation, and galectin-3. Conclusion: Decline in arylsulfatase B leads to transcriptional effects mediated by Sp1 and galectin-3. Significance: Extracellular events can regulate transcription through changes in arylsulfatase B and chondroitin 4-sulfation.

show abstract

Section: Discussionmentioning

confidence: 84%

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Bhattacharyya

Feferman

Tobacman

2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the drug used as reference, dexamethasone, was more effective in preventing these effects. It has been recently shown that carrageenan activates the tolllike receptor 4 (TLR4) (Bhattacharyya et al 2008). TLR4 is an important receptor in the initiation of the inflammatory response in human cells (Lin and Yeh 2005).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil

Silva

Luft

Lunardelli

et al. 2015

An. Acad. Bras. Ciênc.

135

View full text Add to dashboard Cite

Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The inflammatory response evoked by carrageenan and by croton oil was reduced through the pre-treatment of animals with LEO. In the pleurisy model, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to that observed for dexamethasone. In the formalin test, LEO consistently inhibited spontaneous nociception and presented a similar effect to that of tramadol. The results of this study reveal (in vivo) the analgesic and anti-inflammatory activities of LEO and demonstrates its important therapeutic potential.

show abstract

“…Activation of this cascade induced the nuclear translocation of NFκB (p65) and enhanced IL-8 secretion in NCM460 cells and normal human colonocytes in tissue culture. Recently, we identified Toll-like receptor 4 (TLR4) as the cell surface receptor for this innate immune pathway that is stimulated by CGN and mediated by Bcl10 [6].…”

Section: Introductionmentioning

confidence: 99%

Carrageenan-induced NFκB activation depends on distinct pathways mediated by reactive oxygen species and Hsp27 or by Bcl10

Bhattacharyya

Dudeja

Tobacman

2008

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

Carrageenans are highly sulfated polysaccharides that are widely used as food additives due to their ability to improve food texture. They are also widely recognized for their ability to induce inflammation in animal models of colitis. Recently, we reported that carrageenan (CGN) activated a pathway of innate immunity in human colonic epithelial cells mediated by Bcl10 (B-cell CLL/ lymphoma 10). However, increases in phospho-IκBα and Interleukin-8 (IL-8) were not completely inhibited by silencing Bcl10, suggesting that CGN also influenced another mechanism, or mechanisms, of inflammation. In this report, we demonstrate that CGN increases production of reactive oxygen species (ROS) in human colonic epithelial cells. The combination of ROS quenching by the free radical scavenger Tempol and of Bcl10 silencing by siRNA completely inhibited the CGN-induced increases in nuclear NFκB (p65), phospho-IκBα, and secretion of IL-8. The CGN-induced increase in ROS was associated with declines in phosphorylation of MAPK 12 (p38γ), MAPK 13 (p38δ), and heat-shock protein (Hsp) 27. The CGN-induced decline in phospho-Hsp27 was reversed by co-administration of Tempol (100nM), but unaffected by silencing Bcl10. Since Hsp27 phosphorylation is inversely associated with phosphorylation of the IκBα kinase (IKK) signalosome, CGN exposure appears to affect the IKK signalosome by both the catalytic component, mediated by ROS-phospho-Hsp27, and the regulatory component, mediated by Bcl10 interaction with IKKγ (Nemo). Hence, the CGN-activated inflammatory cascades related to innate immunity and to generation of ROS may be integrated at the level of the IKK signalosome.

show abstract

Toll-like Receptor 4 Mediates Induction of the Bcl10-NFκB-Interleukin-8 Inflammatory Pathway by Carrageenan in Human Intestinal Epithelial Cells

Cited by 152 publications

References 48 publications

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Increased Expression of Colonic Wnt9A through Sp1-mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin 4-Sulfate, and Galectin-3

Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil

Carrageenan-induced NFκB activation depends on distinct pathways mediated by reactive oxygen species and Hsp27 or by Bcl10

Contact Info

Product

Resources

About