Toll-pathway in tiger shrimp (Penaeus monodon) responds to white spot syndrome virus infection: Evidence through molecular characterisation and expression profiles of MyD88, TRAF6 and TLR genes

Deepika, A.; Sreedharan, K.; Paria, Anutosh; Makesh, M.; Rajendran, K. V.

doi:10.1016/j.fsi.2014.09.026

Cited by 96 publications

(42 citation statements)

References 62 publications

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“…This general pattern of only a moderate regulation of tlr expression during infection in O. mykiss contrasts reports from other organisms. Pronounced upregulation of tlr expression after infection with relevant pathogens has been documented for mammals [21,23], invertebrates [45,46], and also bony fish. Distinct sets of tlr genes showed enhanced expression in channel catfish Ictalurus punctatus [19], Atlantic salmon Salmo salar [18], and Antarctic bullhead notothen Notothenia coriiceps [20] after parasitic, bacterial, and viral infection, respectively.…”

Section: Discussionmentioning

confidence: 99%

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Brietzke

Korytář

Jaros

et al. 2015

Journal of Immunology Research

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Toll-like receptors (TLRs) are known to detect a defined spectrum of microbial structures. However, the knowledge about the specificity of teleost Tlr factors for distinct pathogens is limited so far. We measured baseline expression profiles of 18 tlr genes and associated signaling factors in four immune-relevant tissues of rainbow trout Oncorhynchus mykiss. Intraperitoneal injection of a lethal dose of Aeromonas salmonicida subsp. salmonicida induced highly increased levels of cytokine mRNAs during a 72-hour postinfection (hpi) period. In contrast, only the fish-specific tlr22a2 and the downstream factor irak1 featured clearly increased transcript levels, while the mRNA concentrations of many other tlr genes decreased. Flow cytometry quantified cell trafficking after infection indicating a dramatic influx of myeloid cells into the peritoneum and a belated low level immigration of lymphoid cells. T and B lymphocytes were differentiated with RT-qPCR revealing that B lymphocytes emigrated from and T lymphocytes immigrated into head kidney. In conclusion, no specific TLR can be singled out as a dominant receptor for A. salmonicida. The recruitment of cellular factors of innate immunity rather than induced expression of pathogen receptors is hence of key importance for mounting a first immune defense against invading A. salmonicida.

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Section: Discussionmentioning

confidence: 99%

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Brietzke

Korytář

Jaros

et al. 2015

Journal of Immunology Research

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“…In shrimp, the key molecules of the Toll pathway have been identified, such as Spätzle, Toll receptors, MyD88, Pelle, Cactus, and Dorsal (25)(26)(27)(28)(29)(30)(31), and reviewed briefly for the signal pathway by Li and Xiang (32). Toll-interacting protein (Tollip) from Litopenaeus vannamei was reported to negatively regulate the shrimp antimicrobial peptide gene, penaeidin-4 (PEN4) (33).…”

Section: (3)mentioning

confidence: 99%

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

Sun

Lan

Shi

et al. 2016

Journal of Biological Chemistry

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The Toll signaling pathway plays an important role in the innate immunity of Drosophila melanogaster and mammals. The activation and termination of Toll signaling are finely regulated in these animals. Although the primary components of the Toll pathway were identified in shrimp, the functions and regulation of the pathway are seldom studied. We first demonstrated that the Toll signaling pathway plays a central role in host defense against Staphylococcus aureus by regulating expression of antimicrobial peptides in shrimp. We then found that ␤-arrestins negatively regulate Toll signaling in two different ways. ␤-Arrestins interact with the C-terminal PEST domain of Cactus through the arrestin-N domain, and Cactus interacts with the RHD domain of Dorsal via the ankyrin repeats domain, forming a heterotrimeric complex of ␤-arrestin⅐Cactus⅐ Dorsal, with Cactus as the bridge. This complex prevents Cactus phosphorylation and degradation, as well as Dorsal translocation into the nucleus, thus inhibiting activation of the Toll signaling pathway. ␤-Arrestins also interact with non-phosphorylated ERK (extracellular signal-regulated protein kinase) through the arrestin-C domain to inhibit ERK phosphorylation, which affects Dorsal translocation into the nucleus and phosphorylation of Dorsal at Ser 276 that impairs Dorsal transcriptional activity. Our study suggests that ␤-arrestins negatively regulate the Toll signaling pathway by preventing Dorsal translocation and inhibiting Dorsal phosphorylation and transcriptional activity.

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“…Shrimp were kept in 500-I FRP tanks containing sea water (14 ± 2 ppt salinity) with continuous aeration and maintained at 25–30°C. The collected animals were fed on commercially available pellet diet and acclimatized for 4–5 days before the experiment 30 . Hepatopancreas was collected from a group of three to four animals and pooled together for the experiments (Fig.…”

Section: Methodsmentioning

confidence: 99%

DNA double-strand break repair inPenaeus monodonis predominantly dependent on homologous recombination

Srivastava

Dahal

Naidu

et al. 2017

DNA Res

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DNA double-strand breaks (DSBs) are mostly repaired by nonhomologous end joining (NHEJ) and homologous recombination (HR) in higher eukaryotes. In contrast, HR-mediated DSB repair is the major double-strand break repair pathway in lower order organisms such as bacteria and yeast. Penaeus monodon, commonly known as black tiger shrimp, is one of the economically important crustaceans facing large-scale mortality due to exposure to infectious diseases. The animals can also get exposed to chemical mutagens under the culture conditions as well as in wild. Although DSB repair mechanisms have been described in mammals and some invertebrates, its mechanism is unknown in the shrimp species. In the present study, we show that HR-mediated DSB repair is the predominant mode of repair in P. monodon. Robust repair was observed at a temperature of 30 °C, when 2 µg of cell-free extract derived from hepatopancreas was used for the study. Although HR occurred through both reciprocal recombination and gene conversion, the latter was predominant when the bacterial colonies containing recombinants were evaluated. Unlike mammals, NHEJ-mediated DSB repair was undetectable in P. monodon. However, we could detect evidence for an alternative mode of NHEJ that uses microhomology, termed as microhomology-mediated end joining (MMEJ). Interestingly, unlike HR, MMEJ was predominant at lower temperatures. Therefore, the results suggest that, while HR is major DSB repair pathway in shrimp, MMEJ also plays a role in ensuring the continuity and stability of the genome.

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Toll-pathway in tiger shrimp (Penaeus monodon) responds to white spot syndrome virus infection: Evidence through molecular characterisation and expression profiles of MyD88, TRAF6 and TLR genes

Cited by 96 publications

References 62 publications

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

DNA double-strand break repair inPenaeus monodonis predominantly dependent on homologous recombination

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