Tolloid-like 1 is negatively regulated by stress and glucocorticoids

Tamura, Goichiro; Olson, Dawne; Miron, Joel; Clark, Timothy G.

doi:10.1016/j.molbrainres.2005.09.016

Cited by 17 publications

(30 citation statements)

References 77 publications

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“…Thus transcription of TLL1 may be regulated by SMAD and/or NF-kappaB signaling during periods of muscle adaptation. In a previous study, the TLL1 promoter was shown to contain consensus binding sites for the glucocorticoid receptor, as well as for the heart-specific transcription factor Nkx2-5 and the brain-specific transcription factor MyT1 [24], which may suggest that its expression is regulated by these elements in these other tissues.…”

Section: Discussionmentioning

confidence: 95%

Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation

et al. 2010

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Members of the bone morphogenetic protein-1/mammalian tolloid (BMP-1/mTLD) family of proteases cleave diverse extracellular proteins, including the growth inhibitor myostatin. The purpose of this work was to examine the expression of BMP-1/mTLD, tolloid-like-1 and -2 (TLL1 and TLL2) in hindlimb muscles of the mouse in vivo and in C(2)C(12) muscle cells in vitro. Quantitative real-time polymerase chain reaction revealed that neither BMP-1/mTLD nor TLL1 mRNA levels differed between the predominantly fast-twitch tibialis anterior (TA) and gastrocnemius (GAST) muscles and the more slow-twitch soleus (SOL) muscle; TLL2 mRNA levels were not detectable in any of the muscles examined. Interestingly, however, immunohistochemical analysis revealed that BMP-1 protein was expressed in type I and IIa but not in IIb fibers. TLL1 mRNA levels significantly increased in the TA but not the SOL with 3 days of hindlimb suspension and significantly decreased in both TA and SOL in response to 2 days of food deprivation. In contrast, BMP-1/mTLD mRNA levels were unaffected in either muscle by either condition. In addition, BMP-1/mTLD and TLL1 mRNA levels significantly decreased during C(2)C(12) myoblast differentiation in vitro, and activity of a 1,200-bp mouse TLL1 promoter construct was significantly decreased in C(2)C(12) myotubes by differentiation, by mutation of an nuclear factor kappa-beta (NF-kappaB) site, or deletion of a sma/mothers against decapentaplegic (SMAD) site. Together, these data demonstrate that TLL1 mRNA levels are altered by loading, energy status, and differentiation, and thus its expression may be regulated so as to modulate activity of myostatin or other extracellular substrates during these adaptive states.

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Section: Discussionmentioning

confidence: 95%

Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation

et al. 2010

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“…For example, one isoform of tolloid-like protein 1 (Tll1) was more highly expressed in females, while another isoform was higher in males. Tll1 has been linked to sex differences in behavioural response to stress in mice42 and, based on the current data it may be of interest to further define the role of specific isoforms of this gene in both males and females. The consistency of sexual dimorphism in our hamster transcriptome compared with other species indicates that this de novo assembly will be a powerful toolkit for future use in hypothesis-driven investigation of gene expression in male and female hamsters.…”

Section: Resultsmentioning

confidence: 94%

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters

McCann

Sinkiewicz

Norvelle

et al. 2017

Sci Rep

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Hamsters are an ideal animal model for a variety of biomedical research areas such as cancer, virology, circadian rhythms, and behavioural neuroscience. The use of hamsters has declined, however, most likely due to the dearth of genetic tools available for these animals. Our laboratory uses hamsters to study acute social stress, and we are beginning to investigate the genetic mechanisms subserving defeat-induced behavioural change. We have been limited, however, by the lack of genetic resources available for hamsters. In this study, we sequenced the brain transcriptome of male and female Syrian hamsters to generate the necessary resources to continue our research. We completed a de novo assembly and after assembly optimization, there were 113,329 transcripts representing 14,530 unique genes. This study is the first to characterize transcript expression in both female and male hamster brains and offers invaluable information to promote understanding of a host of important biomedical research questions for which hamsters are an excellent model.

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“…Evidence suggests that the Tolloids facilitate the activity of neurotrophic factors, such as the BMPs, and may function in regulation of neurogenesis and mechanisms of synaptic plasticity. Tamura et al (2005) found a significantly greater number of Tll-1 expressing cells in the hippocampus of mice provided access to running wheels than in non-running controls, conditions in which neurogenesis is enhanced (van Praag et al 1999;Tamura et al 2005). Moreover, they described novel glucocorticoid response elements within the Tll-1 promotor and that Tll-1 expression is negatively regulated by glucocortcoids.…”

Section: Discussionmentioning

confidence: 97%

“…In situ hybridization was performed as described in Tamura et al, 2005. Briefly, turtle brainstem preparations were fixed overnight in 4% paraformaldehyde, dehydrated in graded ethanols, and embedded in paraffin.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Further examination of the expression pattern of TLL-1 in normal mice revealed Tll-1 mRNA in the hippocampus in a punctate pattern (Scott et al 2000). Characterization of Tll-1 mRNA expression showed that the number of positive cells were increased in the hippocampus of mice with access to running wheels, conditions that increase neurogenesis, and were reduced in the hippocampus of mice under conditions that decrease neurogenesis (Tamura et al 2005). Since mice with increased neurogenesis show improved learning and memory (Gould and Tanapat 1999) it is possible that Tll-1 plays a role in this process.…”

Section: Introductionmentioning

confidence: 98%

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Characterization of a novel reptilian tolloid-like gene in the pond turtle, Pseudemys scripta elegans

Sabirzhanov¹,

Keifer²,

Clark³

2007

Brain Research

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The Tolloid metalloproteases are pleiotropic enzymes that are important for many developmental processes. This study describes the isolation and characterization of a novel Tolloid family member from the pond turtle Pseudemys scripta elegans. The turtle Tolloid, designated tTll, is found in two forms. The first, tTlls, contains a signal sequence which may provide a mechanism for secretion. The second, tTllc, does not contain a signal sequence and is likely cytoplasmic. Sequence analysis of tTll revealed that it is most closely related to chicken Tll-2 although tTll domain structure is different. We examined the expression of tTll mRNA by real time RT-PCR and found the highest expression in the cerebellum with lower levels in the brain stem and cortex. This expression pattern is similar to the expression of the Tolloid mouse orthologues Tll-1 and Tll-2 with highest levels of expression in the cerebellum and lower levels in the brain stem and cortex.

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Tolloid-like 1 is negatively regulated by stress and glucocorticoids

Cited by 17 publications

References 77 publications

Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation

Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation

De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters

Characterization of a novel reptilian tolloid-like gene in the pond turtle, Pseudemys scripta elegans

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