Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Jiang, Xuewei; Fan, Zhichao; Yu, Yili; Shao, Chenying; Suo, Yuanzhen; Wei, Xunbin; Zhou, Yi

doi:10.1142/s1793545815500170

Cited by 12 publications

(12 citation statements)

References 22 publications

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“…43 The potential use of blue light sources (405, 415, 407 to 420 nm) for phototherapy of lesions caused by growth of pathological bacteria is discussed in the literature. 2,[16][17][18][19][20][21][22][25][26][27][28][29][30]44…”

Section: Resultsmentioning

confidence: 99%

“…24 Inactivation of oral bacteria by visible light has been reported elsewhere. 13,15,19,[25][26][27][28][29] The killing efficiency of 405-nm LED light for Propionibacterium acnes and Staphylococcus epidermidis at constant doses of 35, 70, and 144 J∕cm 2 with five different irradiation times from 30 to 240 min has been studied in Ref. 30.…”

Section: Introductionmentioning

confidence: 99%

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Adjunctive dental therapy via tooth plaque reduction and gingivitis treatment by blue light-emitting diodes tooth brushing

et al. 2015

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The efficacy of blue light-emitting toothbrushes (B-LETBs) (405 to 420 nm, power density 2 mW∕cm 2) for reduction of dental plaques and gingival inflammation has been evaluated. Microbiological study has shown the multifactor therapeutic action of the B-LETBs on oral pathological microflora: in addition to partial mechanical removal of bacteria, photodynamic action suppresses them up to 97.5%. In the pilot clinical studies, subjects with mild to moderate gingivitis have been randomly divided into two groups: a treatment group that used the B-LETBs and a control group that used standard toothbrushes. Indices of plaque, gingival bleeding, and inflammation have been evaluated. A significant improvement of all dental indices in comparison with the baseline (by 59%, 66%, and 82% for plaque, gingival bleeding, and inflammation, respectively) has been found. The treatment group has demonstrated up to 50% improvement relative to the control group. We have proposed the B-LETBs to serve for prevention of gingivitis or as an alternative to conventional antibiotic treatment of this disease due to their effectiveness and the absence of drug side effects and bacterial resistance.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adjunctive dental therapy via tooth plaque reduction and gingivitis treatment by blue light-emitting diodes tooth brushing

et al. 2015

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“…Toluidine blue O and porphyrin were successfully tested in bacteria ( Prevotella melaninogenica , Porphyromonas gingivalis , and Aggregatibacter actinomycetemcomitans ) responsible for periodontitis, an infection of the gums [43]. By means of portable LED illumination, survival rate of the three Gram-negative strains could be reduced significantly, although no complete disinfection was achieved.…”

Section: Discussionmentioning

confidence: 99%

What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

et al. 2019

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Due to the increasing development of antibiotic resistances in recent years, scientists search intensely for new methods to control bacteria. Photodynamic treatment with porphyrins such as chlorophyll derivatives is one of the most promising methods to handle bacterial infestation, but their use is dependent on illumination and they seem to be more effective against Gram-positive bacteria than against Gram-negatives. In this study, we tested chlorophyllin against three bacterial model strains, the Gram-positive Bacillus subtilis 168, the Gram-negative Escherichia coli DH5α and E. coli strain NR698 which has a deficient outer membrane, simulating a Gram-negative “without” its outer membrane. Illuminated with a standardized light intensity of 12 mW/cm2, B. subtilis showed high sensitivity already at low chlorophyllin concentrations (≤105 cfu/mL: ≤0.1 mg/L, 106–108 cfu/mL: 0.5 mg/L), whereas E. coli DH5α was less sensitive (≤105 cfu/mL: 2.5 mg/L, 106 cfu/mL: 5 mg/L, 107–108 cfu/mL: ineffective at ≤25 mg/L chlorophyllin). E. coli NR698 was almost as sensitive as B. subtilis against chlorophyllin, pointing out that the outer membrane plays a significant role in protection against photodynamic chlorophyllin impacts. Interestingly, E. coli NR698 and B. subtilis can also be inactivated by chlorophyllin in darkness, indicating a second, light-independent mode of action. Thus, chlorophyllin seems to be more than a photosensitizer, and a promising substance for the control of bacteria, which deserves further investigation.

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“…Targeting eEF1A1 can combat apoptosis-resistant cancers and melanomas 36 . During 5-ALA based PDT, absorption of photon energy by the photosensitizer (PpIX) contained in tumor tissue induces an oxygen dependent photochemical reaction that most probably results in the generation of toxic reactive singlet oxygen species (ROSs) 42 . The oxidative damageoccurs in close proximity to the site where ROSs formed.…”

Section: Discussionmentioning

confidence: 99%

eEF1A1 binds and enriches protoporphyrin IX in cancer cells in 5-aminolevulinic acid based photodynamic therapy

Fan

Cui

Wei

et al. 2016

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Photodynamic therapy (PDT) with protoporphyrin IX (PpIX), which is endogenously derived from 5-aminolevulinic acid (5-ALA) or its derivatives, is a promising modality for the treatment of both pre-malignant and malignant lesions. However, the mechanisms of how ALA-induced PpIX selectively accumulated in the tumors are not fully elucidated. Here we discovered that eukaryotic translation elongation factor 1 alpha 1 (eEF1A1) interacted with PpIX (with an affinity constant of 2.96 × 106 M−1). Microscopy imaging showed that ALA-induced PpIX was co-localized with eEF1A1 in cancer cells. eEF1A1 was found to enrich ALA-induced PpIX in cells by competitively blocking the downstream bioavailability of PpIX. Taken together, our study discovered eEF1A1 as a novel photosensitizer binding protein, which may play an essential role in the enrichment of ALA-induced PpIX in cancer cells during PDT. These suggested eEF1A1 as a molecular marker to predict the selectivity and efficiency of 5-ALA based PDT in cancer therapy.

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Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Cited by 12 publications

References 22 publications

Adjunctive dental therapy via tooth plaque reduction and gingivitis treatment by blue light-emitting diodes tooth brushing

Adjunctive dental therapy via tooth plaque reduction and gingivitis treatment by blue light-emitting diodes tooth brushing

What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

eEF1A1 binds and enriches protoporphyrin IX in cancer cells in 5-aminolevulinic acid based photodynamic therapy

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