Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO

Chelvanayagam, David K.; Beazley, Lyn

doi:10.1016/s0165-0270(96)00155-0

Cited by 11 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, autofluorescence reduction was less effective compared to the Sudan black B protocol, which was particularly evident in zymogen granule‐rich tissue areas (Table ). Toluidine blue has been described to fluoresce at the red end of the spectrum . Accordingly, we observed a clear enhancement of fluorescence in such treated tissue with Cy3 and Texas red filters, but also with the DAPI filter set.…”

Section: Discussionsupporting

confidence: 61%

“…This treatment was found to be not beneficial, due primarily to adverse effects on immunolabelling. Toluidine blue dye has been used widely as a counterstain in combination with immunohistochemistry labelling . Indeed, toluidine blue treatment was helpful to suppress autofluorescence of acinar cells, connective tissue and elastic fibres of blood vessel walls if observations were limited to the FITC filter set.…”

Section: Discussionmentioning

confidence: 99%

“…Toluidine blue dye has been used widely as a counterstain in combination with immunohistochemistry labelling. 40 Indeed, toluidine blue treatment was helpful to suppress autofluorescence of acinar cells, connective tissue and elastic fibres of blood vessel walls if observations were limited to the FITC filter set. However, autofluorescence reduction was less effective compared to the Sudan black B protocol, which was particularly evident in zymogen granule-rich tissue areas ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

What to do with high autofluorescence background in pancreatic tissues – an efficient Sudan black B quenching method for specific immunofluorescence labelling

Erben¹,

Ossig²,

Naim³

et al. 2016

Histopathology

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

What to do with high autofluorescence background in pancreatic tissues – an efficient Sudan black B quenching method for specific immunofluorescence labelling

Erben¹,

Ossig²,

Naim³

et al. 2016

Histopathology

View full text Add to dashboard Cite

show abstract

“…In particular, we show mapping of a cationic thiazine dye toluidine blue O (TBO) at both the cellular and tissue levels. Having a selective affinity for cancer cells in vivo, TBO is a photosensitizer in photodynamic therapy 23,24 .…”

mentioning

confidence: 99%

“…However, it is difficult to image the true TBO distribution, because its fluorescence is quenched when bound to tissue substrates and only the nonspecific stain residue in the tissue retains its native fluorescence 24 . Independent of the complication from fluorescence contrast, stimulated emission microscopy is suitable for this problem.…”

mentioning

confidence: 99%

Imaging chromophores with undetectable fluorescence by stimulated emission microscopy

Min

Lü

Chong

et al. 2009

Nature

240

211

View full text Add to dashboard Cite

Axon order in the visual pathway of the quokka wallaby

1998

View full text Add to dashboard Cite

Axon order throughout the visual pathway of the quokka wallaby (Setonix brachyurus) was determined after localised retinal applications of the tracers DiI and/or DiASP. Postnatal days (P) 22-90 were studied to encompass the development and refinement of retinal projections. Order was essentially similar at all stages. Axons entered the optic nerve head true to their sector of retinal origin. In the optic nerve, nasal and temporal axons continued to reflect their retinal origin, dominating, respectively, the medial and lateral halves. By contrast, dorsal and ventral axons exchanged locations between the retrobulbar level and one-third the distance along the nerve; thus, the inversion of the dorsoventral retinal axis, imposed by the lens, was corrected. Decussating axons maintained their relative locations through the chiasm. At the base of the optic tract, nasal and temporal axons underwent an axial rotation to lie on the medial and lateral sides, respectively; thus nasal overlapped with ventral axons and temporal with dorsal axons. Axons maintained their alignments throughout the tract, and as a result, nasal and ventral axons invaded the superior colliculus medially, whereas temporal and dorsal axons invaded laterally. Each retinal quadrant terminated preferentially in its retinotopically appropriate sector of the colliculus. The arrangement of axons in the quokka visual pathway displays several novel features. Axon order is distinct throughout, involving a well-demarcated exchange of dorsal and ventral axons in the nerve and an axial rotation of nasal and temporal axons at the base of the tract; these relocations suggest decision regions for growing axons. The organisation presumably underlies the less extensive searching within the developing superior colliculus to generate retinotopic maps in the quokka and also in tammar wallaby [Marotte, J. Comp Neurol. 293:524-539, 1990] than in the rat [Simon and O'Leary, J. Neurosci. 12:1212-1232, 1992].

show abstract

Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO

Cited by 11 publications

References 27 publications

What to do with high autofluorescence background in pancreatic tissues – an efficient Sudan black B quenching method for specific immunofluorescence labelling

What to do with high autofluorescence background in pancreatic tissues – an efficient Sudan black B quenching method for specific immunofluorescence labelling

Imaging chromophores with undetectable fluorescence by stimulated emission microscopy

Axon order in the visual pathway of the quokka wallaby

Contact Info

Product

Resources

About