Tomato Fruit and Seed Colonization by Clavibacter michiganensis subsp. michiganensis through External and Internal Routes

Tancos, Matthew A.; Chalupowicz, Laura; Barash, Isaac; Manulis-Sasson, Shulamit; Smart, Christine D.

doi:10.1128/aem.02495-13

Cited by 72 publications

(54 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, analysis of overlap among microbiota members across plant compartments or generations planted in the field is not sufficient to prove vertical transmission, even if seeds are specifically investigated. Tracking studies of GFP-labeled bacteria entering the seed from the parent plant [52,53] offer one of the most precise methods for determining vertical transmission, though it requires genetic manipulation of strains and tools that largely are unavailable for yetuncultivable, non-model microorganisms.…”

Section: Current Opinion In Microbiologymentioning

confidence: 99%

Ecological patterns of seed microbiome diversity, transmission, and assembly

Shade

Martin

Barret

2017

Current Opinion in Microbiology

344

280

View full text Add to dashboard Cite

Seeds are involved in the transmission of microorganisms from one plant generation to another and consequently act as the initial inoculum for the plant microbiota. The purpose of this mini-review is to provide an overview of current knowledge on the diversity, structure and role of the seed microbiota. The relative importance of the mode of transmission (vertical vs horizontal) of the microbial entities composing the seed microbiota as well as the potential connections existing between seed and other plant habitats such as the anthosphere and the spermosphere is discussed. Finally the governing processes (niche vs neutral) involved in the assembly and the dynamics of the seed microbiota are examined.

show abstract

Section: Current Opinion In Microbiologymentioning

confidence: 99%

Ecological patterns of seed microbiome diversity, transmission, and assembly

Shade

Martin

Barret

2017

Current Opinion in Microbiology

344

280

View full text Add to dashboard Cite

show abstract

“…All of the plant pathogenic bacteria are usually Gram negative except Clavibacter and Streptomyces [32] ; [33]. All four tomato strains of R. solanacearum (Maseno, Mariwa, Seme and Holo) from Maseno region were…”

Section: Gram Stainmentioning

confidence: 99%

Isolation and Characterisation of Ralstonia Solanacearum Strains of Tomato Wilt Disease from Maseno, Kenya

Khasabulli

Musyimi

Miruka

et al. 2017

Journal of Asian Scientific Research

View full text Add to dashboard Cite

Article HistoryRalstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5-1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants.

show abstract

“…tomato enter the fruit via glandular and nonglandular trichomes. Pseudomonas syringae as well as, Salmonella enterica are known to colonize tomato leaf trichomes (Tancos et al 2013;Schneider and Grogan 1977;Barak et al 2011;Getz et al 1983). Relatively little is known about the mechanisms by which Fusarium species penetrates and colonizes the maize leaf surface.…”

Section: Introductionmentioning

confidence: 97%