Tomato fruit development through the perspective of transcription factors

Singh, Vigyasa; Phukan, Dharitree; Phukan, Ujjal J.

doi:10.1016/b978-0-323-90613-5.00011-x

Cited by 1 publication

(1 citation statement)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A MADS‐box transcription factor, SlTAGL1 plays a key role in regulating fruit ripening and flavonoid metabolism (Itkin et al., 2009; Singh et al., 2023; Vrebalov et al., 2009). Since the transcript levels of SlTAGL1 and SlDQD/SDH2 were highly correlated in fruits by RNA‐seq analysis (Li et al., 2020) (Figure S3), and the promoter of SlDQD/SDH2 harbors a CArG‐box motif (CCAAAAAGG), characteristic of SlTAGL1 gene targets, we hypothesized that SlDQD/SDH2 may act as a target of SlTAGL1.…”

Section: Resultsmentioning

confidence: 99%

The MADS‐box protein SlTAGL1 regulates a ripening‐associated SlDQD/SDH2 involved in flavonoid biosynthesis and resistance against Botrytis cinerea in post‐harvest tomato fruit

et al. 2023

View full text Add to dashboard Cite

SUMMARY3‐Dehydroquinate dehydratase/shikimate dehydrogenase (DQD/SDH) is a key rate‐limiting enzyme that catalyzes the synthesis of the shikimate, which is an important metabolic intermediate in plants and animals. However, the function of SlDQD/SDH family genes in tomato (Solanum lycopersicum) fruit metabolites is still unknown. In the present study, we identified a ripening‐associated SlDQD/SDH member, SlDQD/SDH2, that plays a key role in shikimate and flavonoid metabolism. Overexpression of this gene resulted in an increased content of shikimate and flavonoids, while knockout of this gene by CRISPR/Cas9 mediated gene editing led to a significantly lower content of shikimate and flavonoids by downregulation of flavonoid biosynthesis‐related genes. Moreover, we showed that SlDQD/SDH2 confers resistance against Botrytis cinerea attack in post‐harvest tomato fruit. Dual‐luciferase reporter and EMSA assays indicated that SlDQD/SDH2 is a direct target of the key ripening regulator SlTAGL1. In general, this study provided a new insight into the biosynthesis of flavonoid and B. cinerea resistance in fruit tomatoes.

show abstract