Tomato genetic resistance to tobamoviruses is compromised

Avni, Ben; Gelbart, Dana; Sufrin-Ringwald, Tali; Zinger, Avner; Chen, L.; Machbash, Z.; Bekelman, Ilana; Segoli, M.; Dombrovsky, Aviv; Kamenetsky, Rina; Levin, Ilan; Lapidot, Moshe

doi:10.17660/actahortic.2021.1316.13

Cited by 15 publications

(22 citation statements)

References 19 publications

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“…As these three accessions can also restrict TMV and ToMV to inoculation foci, they have great potential as a source of genetic resistance to tobamoviruses for tomato breeding programmes. Another screening of 160 genotypes by Zinger and colleagues identified one resistant and 29 tolerant genotypes (Zinger et al, 2021). A further inheritance analysis of a selected tolerant genotype and the resistant genotype showed that the tolerance trait is controlled by a single recessive gene whereas the resistance trait is controlled by at least two genes (Zinger et al, 2021).…”

Section: Genetic Resistancementioning

confidence: 99%

“…Another screening of 160 genotypes by Zinger and colleagues identified one resistant and 29 tolerant genotypes (Zinger et al, 2021). A further inheritance analysis of a selected tolerant genotype and the resistant genotype showed that the tolerance trait is controlled by a single recessive gene whereas the resistance trait is controlled by at least two genes (Zinger et al, 2021). One more recent screening in eukaryotes and the primary antiviral response in plants (Guo et al, 2019;Li & Wang, 2019).…”

Section: Genetic Resistancementioning

confidence: 99%

“…Recently, Tomato brown rugose fruit virus (ToBRFV), a new virus in the genus Tobamovirus , has been identified from tomato plants (Luria et al, 2017 ; Salem et al, 2016 ). The virus has caused devastating disease outbreaks in tomato production areas in many countries, resulting in a severe reduction in yield (Avni et al, 2021 ; EPPO, 2020 ; Jones, 2021 ; Oladokun et al, 2019 ). Currently, ToBRFV is considered the most serious threat to tomato production in the world.…”

Section: Introductionmentioning

confidence: 99%

“…Foliar symptoms such as leaf narrowing, chlorotic mottling, and dark green bulges were documented in reports from Germany and the Netherlands (Menzel et al, 2019 ; van de Vossenberg et al, 2020 ), and drying and brown necrosis patches on the pedicles, calyces, and flowers were observed in the United States and China (Chanda et al, 2021b ; Yan et al, 2019 ). Infection by ToBRV can reduce fruit yield by 15%–55% regardless of whether or not tested tomato cultivars carry the resistance gene Tm‐2 2 (Avni et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide

Zhang

Griffiths

Marchand

et al. 2022

Molecular Plant Pathology

101

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Tomato brown rugose fruit virus (ToBRFV) is an emerging and rapidly spreading RNA virus that infects tomato and pepper, with tomato as the primary host. The virus causes severe crop losses and threatens tomato production worldwide. ToBRFV was discovered in greenhouse tomato plants grown in Jordan in spring 2015 and its first outbreak was traced back to 2014 in Israel. To date, the virus has been reported in at least 35 countries across four continents in the world. ToBRFV is transmitted mainly via contaminated seeds and mechanical contact (such as through standard horticultural practices). Given the global nature of the seed production and distribution chain, and ToBRFV's seed transmissibility, the extent of its spread is probably more severe than has been disclosed. ToBRFV can break down genetic resistance to tobamoviruses conferred by R genes Tm‐1 , Tm‐2 , and Tm‐2 2 in tomato and L 1 and L 2 alleles in pepper. Currently, no commercial ToBRFV‐resistant tomato cultivars are available. Integrated pest management‐based measures such as rotation, eradication of infected plants, disinfection of seeds, and chemical treatment of contaminated greenhouses have achieved very limited success. The generation and application of attenuated variants may be a fast and effective approach to protect greenhouse tomato against ToBRFV. Long‐term sustainable control will rely on the development of novel genetic resistance and resistant cultivars, which represents the most effective and environment‐friendly strategy for pathogen control. Taxonomy Tomato brown rugose fruit virus belongs to the genus Tobamovirus , in the family Virgaviridae . The genus also includes several economically important viruses such as Tobacco mosaic virus and Tomato mosaic virus . Genome and virion The ToBRFV genome is a single‐stranded, positive‐sense RNA of approximately 6.4 kb, encoding four open reading frames. The viral genomic RNA is encapsidated into virions that are rod‐shaped and about 300 nm long and 18 nm in diameter. Tobamovirus virions are considered extremely stable and can survive in plant debris or on seed surfaces for long periods of time. Disease symptoms Leaves, particularly young leaves, of tomato plants infected by ToBRFV exhibit mild to severe mosaic symptoms with dark green bulges, narrowness, and deformation. The peduncles and calyces often become necrotic and fail to produce fruit. Yellow blotches, brown or black spots, and rugose wrinkles appear on tomato fruits. In pepper plants, ToBRFV infection results in puckering and yellow mottling on leaves with stunted growth of young seedlings and small yellow to brown rugose do...

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Section: Genetic Resistancementioning

confidence: 99%

Section: Genetic Resistancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide

Zhang

Griffiths

Marchand

et al. 2022

Molecular Plant Pathology

101

View full text Add to dashboard Cite

show abstract

“…Two near-isogenic tomato lines were compared, Moneymaker and Moneymaker harboring the ToMV-resistance gene Tm-2 2 . Depending on the climate and cultivation practices, ToBRFV infection induced yield reductions of 19–55% regardless of the presence of the Tm-2 2 resistance gene [ 27 ]. Due to the threat this emerging virus poses to global tomato cultivation, the USA issued a federal order regarding importing and inspecting tomato seeds, and the European Union has declared a quarantine status for ToBRFV [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

ToBRFV Infects the Reproductive Tissues of Tomato Plants but Is Not Transmitted to the Progenies by Pollination

Avni

Gelbart

Sufrin-Ringwald

et al. 2022

Cells

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Tomato brown rugose fruit virus (ToBRFV), a newly identified Tobamovirus, has recently emerged as a significant pathogen of tomato plants (Solanum lycopersicum). The virus can evade or overcome the known tobamovirus resistance in tomatoes, i.e., Tm-1, Tm-2, and its allele Tm-22. ToBRFV was identified for the first time only a few years ago, and its interactions with the tomato host are still not clear. We investigated ToBRFV’s presence in the reproductive tissues of tomato using fluorescent in situ hybridization (FISH) and RT-PCR. In infected plants, the virus was detected in the leaves, petals, ovary, stamen, style, stigma, and pollen grains but not inside the ovules. Fruits and seeds harvested from infected plants were contaminated with the virus. To test whether the virus is pollen transmitted, clean mother plants were hand pollinated with pollen from ToBRFV-infected plants and grown to fruit. None of the fruits and seeds harvested from the pollinated clean mother plants contained ToBRFV. Pollen germination assays revealed the germination arrest of ToBRFV-infected pollen. We concluded that ToBRFV might infect reproductive organs and pollen grains of tomato but that it is not pollen transmitted.

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Biocompatible antiviral Pickering emulsion‐based formulation for plant root protection from tobamovirus‐infected soil

Mani,

Berenice,

Cohen

et al. 2024

Polymers for Advanced Techs

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The soil‐borne virus known as tomato brown rugose fruit virus (ToBRFV) has a low rate of around 3% soil‐mediated infection when the soil has root debris from a previous 30–50 day development cycle of tomato plants infected with ToBRFV. This study presents anti‐viral coating formulations based on Pickering emulsion. The coating formulation is based on water‐in‐canola oil emulsions stabilized using commercial hydrophobic silica, with water‐soluble polymer (sodium polyacrylic acid). The structure of the emulsions and their stability were characterized by confocal microscopy, centrifugal analysis using a LUMiSizer®, used to confirm the emulsion stability. We tested a few different silica concentration‐based formulations, which were prepared with or without the addition of various virus disinfectants. We found that under conditions of 100% soil‐mediated ToBRFV infection of uncoated positive control plants, root‐coating with formulations based on silica Pickering emulsion were prepared with the disinfectant chlorinated‐trisodium phosphate (TSP‐Cl) showed low percentages of soil‐mediated ToBRFV infection. These formulations had no adverse effect on plant growth parameters when compared to negative control plants grown under non ToBRFV inoculation conditions.

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Tomato genetic resistance to tobamoviruses is compromised

Cited by 15 publications

References 19 publications

Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide

Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide

ToBRFV Infects the Reproductive Tissues of Tomato Plants but Is Not Transmitted to the Progenies by Pollination

Biocompatible antiviral Pickering emulsion‐based formulation for plant root protection from tobamovirus‐infected soil

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