Tomato (Lycopersicon esculentum) allergens in pollen-allergic patients

Skin prick test analysis reveals cross-sensitization to tomato profilin and grass pollen in nasobronchialallergic patients with history of tomato food allergy

“…Understanding the spectrum of cross-reactive pollen-food allergies in different populations is required for the proper diagnosis and treatment of such allergic disorders. The association between grass-pollen sensitization and allergy to tomato fruit is known from studies in Europe and Japan (24)(25)(26)28,30); however, there are no reports of such studies in the Indian population. The present study describes a preliminary investigation based on case history and SPT in a study group of 246 allergic rhinitis / asthma patients in Mysuru city, India, aimed at understanding the importance of tomato profilin as a cross-reactive allergen.…”

“…Possibly connected with the sequence diversity, ubiquity, and abundance of profilins in eukaryotes is the fact that plant profilins are important cross-reactive allergens, and are identified as pan-allergens (23). The prevalence of tomato (Solanum lycopersicum L.) allergy was estimated to range from 1.5% to 16% among food-allergic populations (24)(25)(26)(27), and up to 39.2% among grass pollen-allergic patients (28). Profilin from tomato fruit (14 kDa; Sola l 1) has been identified as a food allergen, and has been cloned and characterized (29,30).…”

“…Foetish et al (28) studied the relevance of tomato allergy in pollen-allergic patients in a group of 32 birch pollen-allergic patients with history of adverse reactions to tomato fruit, and found that tomato allergy occurred with a prevalence of 9% in the study group, and the majority of pollen-associated allergies to tomato were due to ubiquitous allergenic structures such as profilin (44%) and cross-reactive IgE binding N-glycans (35.5%). However, in the present study the sensitization was estimated based only on SPT, in which a positive result shows the presence of mast cell-bound specific IgE to a particular extract or protein.…”

Reduction of Allergenic Proteins by the Effect of theripening inhibitor(rin) Mutant Gene in an F1Hybrid of therinMutant Tomato

“…-Fructofuranosidase and PG-2A have been identified as allergens in tomato fruit, 4,10,21) and Kondo et al 10) have reported that the sera of most patients with OAS to tomato reacted to -fructofuranosidase (9 of 11 patients) and to PG-2A (8 of 11). These results suggest that tomato with a low-level accumulation of -fructofuranosidase and PG-2A reduced the allergic potential towards many patients.…”

“…-Fructofuranosidase had previously been identified as IgE-reacted protein that was detected at 22, 25, 50 and 52-kDa in the SDS-PAGE assay, and PG-2A was also identified at 36-and 46-kDa. 4,10,21) We therefore tried to identify the three protein bands indicated as ''a,'' ''b,'' and ''c'' in Fig. 3A.…”