Tomato molecular biology – special collection of papers for molecular horticulture

Seymour, Graham B.; Rose, Jocelyn K. C.

doi:10.1186/s43897-022-00042-z

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Therefore, the identification and introduction of genetic determinants that were lost during domestication may facilitate the development of salt-tolerant crops ( Eckardt et al ., 2023 ). Tomato ( Solanum lycopersicum L.) is not only an essential crop for its economic importance and high nutritional value, but it is also a model dicot for advancing the understanding of the underlying mechanisms of abiotic stress tolerance ( Seymour and Rose, 2022) . Salinity causes damage to plants by osmotic stress (due to reduced water availability), ionic stress (mainly as a consequence of the toxic effect of Na + ) and accumulation of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato

Egea,

Barragán-Lozano,

Estrada

et al. 2024

Preprint

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Regulation of sodium homeostasis is crucial for plant response to salinity conditions. Here we report on the genetic and physiological characterization of two tomato allelic mutants,sodiumgatherer1-2(sga1-2), which exhibit pronounced chlorosis and hyperhydration under salt stress. Mapping-by-sequencing revealed that mutant phenotype resulted from mutations in theSlRbohGgene, and CRISPR/Cas9 knockouts of this gene gave phenocopies of thesga1-2mutants. Physiological analyses showed thatsga1-2salt hypersensitivity is linked to an increase of Na+and water transport from roots to shoots, which explains their extreme chlorosis and hyperhydration under salinity conditions. At the molecular level,SlPIP2;12gene, an aquaporin down-regulated in the WT under salt stress, was overexpressed in thesga1-2mutants, which could enhance water transport to the shoot. Also,sga1-2mutants exhibited a significant reduction in the expression of key sodium transporters, thus modifying the normal distribution of Na+in tomato plant tissues. Furthermore, treatment of WT plants with the NADPH oxidase inhibitor DPI prevented H2O2production in response to salinity, resulting in elevated Na+accumulation in the shoot and reduced expression of theSlHKT1;2gene in root. Altogether, our results show thatSlRbohGplays a central role in salt tolerance through ROS-mediated signaling.HIGHLIGHTLoss of function of tomatoSlRbohGgene leads hypersensibility to salt stress due to increased Na+and water transport from root to shoot.

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Section: Introductionmentioning

confidence: 99%

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato

Egea,

Barragán-Lozano,

Estrada

et al. 2024

Preprint

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“…Solanum cheesmaniae is one of the two main wild tomato species from the Galápagos Islands with enhanced plant performance against stresses [ 11 ]. Because of its potential stress-tolerance and disease-resistance traits [ 12 ], this species has successfully been used in stress-resistance breeding programs for the improvement of commercial tomato varieties [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Metabolomics of Alternaria solani-Challenged Wild Tomato Species Solanum cheesmaniae Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways

et al. 2023

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Untargeted metabolomics of moderately resistant wild tomato species Solanum cheesmaniae revealed an altered metabolite profile in plant leaves in response to Alternaria solani pathogen. Leaf metabolites were significantly differentiated in non-stressed versus stressed plants. The samples were discriminated not only by the presence/absence of specific metabolites as distinguished markers of infection, but also on the basis of their relative abundance as important concluding factors. Annotation of metabolite features using the Arabidopsis thaliana (KEGG) database revealed 3371 compounds with KEGG identifiers belonging to biosynthetic pathways including secondary metabolites, cofactors, steroids, brassinosteroids, terpernoids, and fatty acids. Annotation using the Solanum lycopersicum database in PLANTCYC PMN revealed significantly upregulated (541) and downregulated (485) features distributed in metabolite classes that appeared to play a crucial role in defense, infection prevention, signaling, plant growth, and plant homeostasis to survive under stress conditions. The orthogonal partial least squares discriminant analysis (OPLS-DA), comprising a significant fold change (≥2.0) with VIP score (≥1.0), showed 34 upregulated biomarker metabolites including 5-phosphoribosylamine, kaur-16-en-18-oic acid, pantothenate, and O-acetyl-L-homoserine, along with 41 downregulated biomarkers. Downregulated metabolite biomarkers were mapped with pathways specifically known for plant defense, suggesting their prominent role in pathogen resistance. These results hold promise for identifying key biomarker metabolites that contribute to disease resistive metabolic traits/biosynthetic routes. This approach can assist in mQTL development for the stress breeding program in tomato against pathogen interactions.

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Utilizing effector-triggered immunity (ETI) as a robust priming agent to protect plants from pathogens

Islam,

Khan,

Chen

et al. 2024

Stress Biology

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Tomato molecular biology – special collection of papers for molecular horticulture

Cited by 3 publications

References 11 publications

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato

Untargeted Metabolomics of Alternaria solani-Challenged Wild Tomato Species Solanum cheesmaniae Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways

Utilizing effector-triggered immunity (ETI) as a robust priming agent to protect plants from pathogens

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Tomato molecular biology – special collection of papers for molecular horticulture

Cited by 3 publications

References 11 publications

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H2O2Production Under Stress for Proper Na+Homeostasis Regulation in Tomato

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H2O2Production Under Stress for Proper Na+Homeostasis Regulation in Tomato

Untargeted Metabolomics of Alternaria solani-Challenged Wild Tomato Species Solanum cheesmaniae Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways

Utilizing effector-triggered immunity (ETI) as a robust priming agent to protect plants from pathogens

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The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato

The Pivotal Involvement of theRespiratory burst oxidase G(SlRbohG) Gene in H₂O₂Production Under Stress for Proper Na⁺Homeostasis Regulation in Tomato