Tomato Mosaic Virus Infection in a Recirculating Nutrient Solution

Pares, R. D.; Gunn, L. V.; Cresswell, G. C.

doi:10.1111/j.1439-0434.1992.tb01266.x

Cited by 30 publications

(19 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the high stability of these pathogens in aqueous environments might allow them to accumulate in and on root systems. Certainly, higher concentrations of PepMV-Ch2, PVY NTN , and PSTVd in the recirculating nutrient solution would result in higher infection rates, as has been shown for tomato mosaic virus by Pares et al (28). We have observed something similar, whereby plants grown in substrate became infected only when watered with high concentrations of PepMV-Ch2 (data not shown).…”

Section: Discussionsupporting

confidence: 68%

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Mehle

Gutiérrez-Aguirre

Prezelj

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

Hydroponic systems and intensive irrigation are used widely in horticulture and thus have the potential for rapid spread of water-transmissible plant pathogens. Numerous plant viruses have been reported to occur in aqueous environments, although information on their survival and transmission is minimal, due mainly to the lack of effective detection methods and to the complexity of the required transmission experiments. We have assessed the role of water as a source of plant infection using three mechanically transmissible plant pathogens that constitute a serious threat to tomato and potato production: pepino mosaic virus (PepMV), potato virus Y (PVY), and potato spindle tuber viroid (PSTVd). PepMV remains infectious in water at 20 ؎ 4°C for up to 3 weeks, PVY (NTN strain) for up to 1 week, and PSTVd for up to 7 weeks. Experiments using a hydroponic system show that PepMV (Ch2 genotype) and PVY (NTN strain) can be released from plant roots into the nutrient solution and can infect healthy plants through their roots, ultimately spreading to the green parts, where they can be detected after a few months. In addition, tubers developed on plants grown in substrate watered with PSTVd-infested water were confirmed to be the source of viroid infection. Our data indicate that although well-known pathways of virus spread are more rapid than water-mediated infection, like insect or mechanical transmission through leaves, water is a route that provides a significant bridge for rapid virus/ viroid spread. Consequently, water should be taken into account in future epidemiology and risk assessment studies.

show abstract

Section: Discussionsupporting

confidence: 68%

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Mehle

Gutiérrez-Aguirre

Prezelj

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Although efficient transmission of plant viruses through nutrient solution in hydroponic systems has been described for plant viruses from different taxa and for different host‐plant species, including tomato [e.g. Tomato bushy stunt tombusvirus, Cymbidium ringspot tombusvirus , Odontoglossum ringspot tobamovirus , Cymbidium mosaic potexvirus and ToMV (Pares et al. , 1992)], the epidemiological relevance of direct transmission through the nutrient solution and transmission by root contact or root‐grafting for virus transmission has rarely been studied.…”

Section: Discussionmentioning

confidence: 99%

“…Cucumber green mottle mosaic virus , Cucumber mosaic virus and Arabis mosaic virus infecting cucumber (Paludan, 1985; Büttner et al. , 1995), Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) infecting pepper and tomato (Paludan, 1985; Pares et al. , 1992; Büttner et al.…”

Section: Introductionmentioning

confidence: 99%

Spread and interaction of Pepino mosaic virus (PepMV) and Pythium aphanidermatum in a closed nutrient solution recirculation system: effects on tomato growth and yield

et al. 2010

View full text Add to dashboard Cite

Pepino mosaic virus (PepMV) was shown to be efficiently transmitted between tomato plants grown in a closed recirculating hydroponic system. PepMV was detected in all plant parts after transmission via contaminated nutrient solution using ELISA, immunocapture RT-PCR, RT-PCR, electron microscopy, and by inoculation to indicator plants. Detection of Pep-MV in nutrient solution was only possible after concentration by ultracentrifugation followed by RT-PCR. Roots tested positive for PepMV 1-3 weeks after inoculation, and subsequently a rapid spread from the roots into the young leaves and developing fruits was found within 1 week. PepMV was only occasionally detected in the older leaves. None of the infected plants showed any symptoms on fruits, leaves or other organs. Pre-infection of roots of tomato cv. Hildares with Pythium aphanidermatum significantly delayed PepMV root infections. When mechanically inoculated with PepMV at the 2-4 leaf stage, yield loss was observed in all plants. However, only plants of cv. Castle Rock recorded significant yield losses when infected via contaminated nutrient solution. Yield losses induced by infection with PepMV and ⁄ or P. aphanidermatum ranged from 0AE4 up to 40% depending on experimental conditions.

show abstract

“…These could also act as sources of inoculum by releasing viruses Table 3 Infection levels of tomato seedlings mechanically root-inoculated with different concentrations of inoculum. Only 1 plant (in the 3"" level) developed symptoms in root efflux (Pares et al, 1992). Thus 68% of plants were actually infected from the soil.…”

Section: Discussionmentioning

confidence: 97%

The Role of Infective Plant Debris, and its Concentration in Soil, in the Ecology of Tomato Mosaic Tobamovirus—a Non‐vectored Plant Virus

Pares

Gunn

Keskula

1996

Journal of Phytopathology

Self Cite

View full text Add to dashboard Cite

Only a few plants in a crop are generally thought to become infected by abiotic soil transmission. In glasshouse experiments we have induced almost total infection in tomatoes growing in soil with infective debris, but levels of infection are dependent upon certain conditions. We found that as the inoculum concentration decreased (i) a greater percentage of the infections were either latent (symptomless) or restricted to roots and (ii) infection levels decreased. Thus, apparent infection (based on symptoms) of 0. 10% was in reality 0 60–70% based on enzyme‐linked immunosorbent assay and immunoelectron microscopy testing of roots and leaves. This occurred whether seedlings or seed were planted into infected mix. Under conditions in which minimal root damage was caused (planting seed) or roots were mechanically inoculated only once, almost all systemically infected plants were symptomless.

show abstract

Tomato Mosaic Virus Infection in a Recirculating Nutrient Solution

Cited by 30 publications

References 8 publications

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Spread and interaction of Pepino mosaic virus (PepMV) and Pythium aphanidermatum in a closed nutrient solution recirculation system: effects on tomato growth and yield

The Role of Infective Plant Debris, and its Concentration in Soil, in the Ecology of Tomato Mosaic Tobamovirus—a Non‐vectored Plant Virus

Contact Info

Product

Resources

About