Tomato R2R3-MYB Proteins SlANT1 and SlAN2: Same Protein Activity, Different Roles

Kiferle, Claudia; Fantini, Elio; Bassolino, Laura; Povero, Giovanni; Spelt, Cornelis; Buti, Sara; Giuliano, Giovanni; Quattrocchio, Francesca; Koes, Ronald; Perata, Pierdomenico; Gonzali, Silvia

doi:10.1371/journal.pone.0136365

Cited by 142 publications

(174 citation statements)

References 65 publications

Supporting

Mentioning

165

Contrasting

Order By: Relevance

“…Through mining the WGBS datasets, we also identified an MYB TF gene SlAN2 which is involved in regulating expression of genes required for anthocyanidin biosynthesis (Povero et al, 2010;Kiferle et al, 2015). Compared to AC and rin, the DMR in the SlAN2 gene promoter was almost completely free of DNA methylation in normal Cnr fruit.…”

Section: Resultsmentioning

confidence: 99%

Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening

Chen

Kong

et al. 2017

Sci. China Life Sci.

View full text Add to dashboard Cite

Whole-genome bisulfite sequencing (WGBS) allows single-base resolution and genome-wide profiling of DNA methylation in plants and animals. This technology provides a powerful tool to identify genes that are potentially controlled by dynamic changes of DNA methylation and demethylation. However, naturally occurring epimutants are rare and genes under epigenetic regulation as well as their biological relevances are often difficult to define. In tomato, fruit development and ripening are a complex process that involves epigenetic control. We have taken the advantage of the tomato epimutant Colourless non-ripening (Cnr) and performed comparative mining of the WGBS datasets for the Cnr and SlCMT3-silenced Cnr fruits. We compared DNA methylation profiles for the promoter sequences of approximately 5,000 bp immediately upstream of the coding region of a list of 20 genes. Differentially methylated regions were found for some of these genes. Virus-induced gene silencing (VIGS) of differentially methylated gene SlDET1 or SlPDS resulted in unusual brown pigmentation in Cnr fruits. These results suggest that comparative WGBS coupled with VIGS can be used to identify genes that may contribute to the colourless unripe phenotype of fruit in the Cnr epimutant.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening

Chen

Kong

et al. 2017

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

“…7A-D), genes related to pigment formation and color, were considerably higher at the fully ripe stage. These increments were positively correlated with the expression of R2R3-Myb [30,31] (Fig. 7 E), the plant transcrip- tion factor which is highly correlated to the anthocyanin accumulation, while the expression of translation elongation factor 1, used as a housekeeping gene, was nearly equal during the all three stages (Fig.…”

Section: Differential Expression Profiles At the Different Fruit Stagesmentioning

confidence: 91%

De novo characterization of the Lycium ruthenicum transcriptome and analysis of its digital gene expression profiles during fruit development and ripening

Peng

Chen

2017

ARCH BIOL SCI BELGRA

View full text Add to dashboard Cite

Lycium ruthenicum Murr., which belongs to the family Solanaceae, is a resource plant for Chinese traditional medicine and nutraceutical foods. In this study, RNA sequencing was applied to obtain raw reads of L. ruthenicum fruit at different stages of ripening, and a de novo assembly of its sequence was performed. Approximately 52.45 million 100bp paired-end raw reads were generated from the samples by deep RNA-seq analysis. These short reads were assembled to obtain 164814 contigs, and the contigs were assembled into 84968 non-redundant unigenes using the Trinity method. Assembled sequences were annotated with gene descriptions, gene ontology, clusters of orthologous group and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway terms. Digital gene expression analysis was applied to compare gene-expression patterns at different fruit developmental stages. These results contribute to existing sequence resources for Lycium spp. during the fruit-ripening stages, which is valuable for further functional studies of genes involved in L. ruthenicum fruit nutraceutical quality.

show abstract

“…For instance, in tomato, MYB transcription factor encoded by two paralogs of tomato's anthocyanin genes; SlANT1 and SlAN2 which has the potential of regulating fruit's anthocyanin pigmentation and color [99]. These two genes induce anthocyanin synthesis in its fruit when their expression is driven by the 35S promoter (35S: SlANT1 and 35S: SlAN2) and both of them have shown the high expression levels of SlDFR which has capable of encoding a key enzyme in anthocyanin biosynthetic pathway.…”

Section: Stmyba1mentioning

confidence: 99%

The Role of Major Transcription Factors in Solanaceous Food Crops under Different Stress Conditions: Current and Future Perspectives

Tolosa

Zhengbin

2020

Plants

View full text Add to dashboard Cite

Plant growth, development, and productivity are adversely affected by environmental stresses such as drought (osmotic stress), soil salinity, cold, oxidative stress, irradiation, and diverse diseases. These impacts are of increasing concern in light of climate change. Noticeably, plants have developed their adaptive mechanism to respond to environmental stresses by transcriptional activation of stress-responsive genes. Among the known transcription factors, DoF, WRKY, MYB, NAC, bZIP, ERF, ARF and HSF are those widely associated with abiotic and biotic stress response in plants. Genome-wide identification and characterization analyses of these transcription factors have been almost completed in major solanaceous food crops, emphasizing these transcription factor families which have much potential for the improvement of yield, stress tolerance, reducing marginal land and increase the water use efficiency of solanaceous crops in arid and semi-arid areas where plant demand more water. Most importantly, transcription factors are proteins that play a key role in improving crop yield under water-deficient areas and a place where the severity of pathogen is very high to withstand the ongoing climate change. Therefore, this review highlights the role of major transcription factors in solanaceous crops, current and future perspectives in improving the crop traits towards abiotic and biotic stress tolerance and beyond. We have tried to accentuate the importance of using genome editing molecular technologies like CRISPR/Cas9, Virus-induced gene silencing and some other methods to improve the plant potential in giving yield under unfavorable environmental conditions.

show abstract

Tomato R2R3-MYB Proteins SlANT1 and SlAN2: Same Protein Activity, Different Roles

Cited by 142 publications

References 65 publications

Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening

Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening

De novo characterization of the Lycium ruthenicum transcriptome and analysis of its digital gene expression profiles during fruit development and ripening

The Role of Major Transcription Factors in Solanaceous Food Crops under Different Stress Conditions: Current and Future Perspectives

Contact Info

Product

Resources

About