Tomato Rab1A Homologs as Molecular Tools for Studying Rab Geranylgeranyl Transferase in Plant Cells

Loraine, Ann E.; Yalovsky, Shaul; Fabry, Stefan; Gruissem, Wilhelm

doi:10.1104/pp.110.4.1337

Cited by 61 publications

(63 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Glu-Glu-tagged proteins can complement a yptl deletion mutant when expressed in yeast, confirming that they are functional YPT1 homologs (Loraine et al, 1996). The canine RablA protein was used as a positive control in the prenylation reactions.…”

Section: Stability Of Ccppmentioning

confidence: 99%

“…Tobacco (Nicotiana tabacum) BY-2 suspension cells (Katsuta and Shibaoka, 1992;Nagata et al, 1992) were subcultured every 7 d into fresh medium containing 4.3 g L-' Murashige-Skoog salts, 100 mg L-' inositol, 1 mg L-' thiamine, 0.2 mg L-' 2,4-D, 255 mg L-l KHPO,, and 30 g L-' SUC. Tomato VFNT Cherry suspension cells were subcultured every 7 d and were grown as described by Loraine et al (1996).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Severa1 Rab proteins have now been cloned from plants (Drew et al, 1993;Nagano et al, 1993;Youssefia:n et al, 1993;Bednarek et al, 1994;Borg and Poulsen, 1994), but little is known about their subcellular localization imd function (Bednarek et al, 1994;Borg and Poulsen, 1994;Park et al, 1994), and their prenylation status has not been addressed. We show that the tomato (Lycopersicon esculentum), YPTl homologs, Rab proteins (LeRablB and LeRablC) (Loraine et al, 1996), and the mammalian RablA serve as substrates for a Rab GGTase in protein extracts made from various plant tissues. Plant Rab GGTase requires GGPP, the wild-type Rab C-terminal prenylation motif, and additional upstream sequences for efficient prenylation of the Rab substrates.…”

mentioning

confidence: 99%

“…In this report we establish the presence in plants of Rab GGTase activity that is specific for prenylation of Rab proteins we have isolated from tomato (Loraine et al, 1996). The plant enzyme attaches C,,-geranylgeranyl to conserved Cys residues in the C termini of the Rab proteins, which are the only known substrates of the mammalian and yeast enzymes (Khosravi-Far et al, 1991;Kins.ella andMaltese, 1991, 1992).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Specific Prenylation of Tomato Rab Proteins by Geranylgeranyl Type-II Transferase Requires a Conserved Cysteine-Cysteine Motif

Yalovsky¹,

Loraine²,

Gruissem³

1996

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Posttranslational isoprenylation of some small CTP-binding proteins is required for their biological activity. Rab geranylgeranyl transferase (Rab CCTase) uses geranylgeranyl pyrophosphate to modify Rab proteins, i t s only known substrates. Ceranylgeranylation of Rabs is believed t o promote their association with target membranes and interaction with other proteins. Plants, like other eukaryotes, contain Rab-like proteins that are associated with intracellular membranes. However, t o our knowledge, the geranylgeranylation of Rab proteins has not yet been characterized from any plant source. This report presents an activity assay that allows the characterization of prenylation of Rab-like proteins in vitro, by protein extracts prepared from plants. Tomato Rabl proteins and mammalian Rabl a were modified by geranylgeranyl pyrophosphate but not by farnesyl pyrophosphate. This modification required a conserved cysteine-cysteine motif. A mutant form lacking the cysteine-cysteine motif could not be modified, but inhibited the geranylgeranylation of its wild-type homolog. The tomato Rab proteins were modified in vitro by protein extract prepared from yeast, but failed to become modified when the protein extract was prepared from a yeast strain containing a mutant allele for the (Y subunit of yeast Rab CGTase (bet4 ts). These results demonstrate that plant cells, like other eukaryotes, contain Rab CCTase-like activity.

show abstract

Section: Stability Of Ccppmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Specific Prenylation of Tomato Rab Proteins by Geranylgeranyl Type-II Transferase Requires a Conserved Cysteine-Cysteine Motif

Yalovsky¹,

Loraine²,

Gruissem³

1996

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Conversely, farnesyl (15-C) or has not been determined. geranylgeranyl (20-C), isoprenoid prodOver the past few years, changes in the ucts of the mevalonic acid (MVA) pathway, profiles of prenylated proteins during plant are attached to C-proximal cysteine development and the growth of suspension-cultured cells have been documented (Randall et al, 1993;Morehead et al, 1995), and more extensive analyses of the potential role of protein prenyltransferases in regulating progression through the plant cell cycle have been reported (Morehead et al, 1995;Lorraine et al, 1996;Schmitt et al, 1996;Yalovsky et al, 1996).…”

mentioning

confidence: 99%