Tomato sucrose transporter SlSUT4 participates in flowering regulation by modulating gibberellin biosynthesis

Bai, Jing; Xie, Zhilong; Lian, Zhaoyuan; Guo, Jia; Zhao, Feiyang; Liang, Yan; Huo, Heqiang; Gong, Haijun

doi:10.1093/plphys/kiad162

Cited by 22 publications

(6 citation statements)

References 81 publications

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“…Sucrose application at 50 mM promoted flowering even under the night break condition of the short day-induced Chrysanthemum 'Flora Yuuka' (Sun et al 2017). Increasing sucrose in the shoot apex by silencing the sucrose transporter in tomato (Solanum lycopersicum) enhanced flowering (Liang et al 2023). Silencing the TREHALOSE-6-PHOSPHATE SYNTHASE 1 (TPS1), the synthase for sugar signal, delayed the flowering of Arabidopsis thaliana under an inductive photoperiod (Wahl et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

Lin,

Chang

2024

horts

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Oncidesa Gower Ramsey ‘Honey Angel’ is a cut flower crop of high economic value worldwide. The regulation of flowering is important for cut flower production scheduling. However, its flowering transition mechanism is still unclear. Oncidesa usually flowers at the end of the growth cycle for each pseudobulb; this timing is probably related to carbohydrate accumulation. During this study, we investigated the carbohydrates in the pseudobulbs from juvenile plants to adult plants and compared the carbohydrates in flowering and nonflowering adult plants. The current pseudobulb and back pseudobulbs of the plants at 0, 0.5, 1.0, 1.5, and 2.0 years after having been moved out of the tissue culture flask were collected. The first pseudobulb formed at 0.5 years, and plants had fulfilled four growth cycles and flowered at 2.0 years. Each successive current shoot grew larger and the back shoot number progressively increased after each new growth cycle. The concentration of total soluble sugars in the current shoot increased from 5.5% of dry weight at 0.5 years to 20.2% of dry weight at 1.5 years. Conversely, the starch concentration decreased in the current pseudobulb as the plants matured. The starch concentration in the back pseudobulbs did not change when the plant grew a new shoot. The starch concentrations in the back pseudobulbs ranged from 33.2% to 57.5% of dry weight, but the combined content of starch in all of the back pseudobulbs increased significantly from 168 mg at 0.5 years to 4608 mg at 2.0 years because of the increasing number of back shoots. The starch in the first back pseudobulb of the nonflowering adult plants accounted for 18.0% of dry weight, which was lower than that of the flowering plants (48.3%). There was no significant difference in total soluble sugars in the current pseudobulb of the nonflowering and flowering plants. Overall, we revealed that the increase in the back shoot number increased the total amount of reserve carbohydrates as the plant reached reproductive maturity. A low starch level was observed in nonflowering adult plants. In both cases, flowering plants had higher starch storage in the back pseudobulbs, suggesting that carbohydrates might regulate the flowering of Oncidesa Gower Ramsey ‘Honey Angel’.

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Section: Discussionmentioning

confidence: 99%

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

Lin,

Chang

2024

horts

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“…The BL21 bacteria were transformed with the fusion protein, and induction was carried out using constant shaking at 200 r/min and a temperature of 28°C for 8 h with an IPTG concentration of 0.5 m m . Liang's methodology for EMSA was followed (Liang et al., 2023). The mutation and cold probes employed for EMSA are found in Table S3.…”

Section: Methodsmentioning

confidence: 99%

“…The SlWRKY81 's cDNA was cloned into the pGADT7 vector, and each fragment of the SlSPDS2 promoter and SlNHX4 promoter was individually inserted into the pAbAi vector. After that, Liang's methodology for Y1H was followed (Liang et al., 2023). Table S1 lists the related primers.…”

Section: Methodsmentioning

confidence: 99%

SlWRKY81 regulates Spd synthesis and Na⁺/K⁺ homeostasis through interaction with SlJAZ1 mediated JA pathway to improve tomato saline‐alkali resistance

Shang,

Liu,

Chen

et al. 2024

The Plant Journal

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SUMMARYSaline‐alkali stress is an important abiotic stress factor affecting tomato (Solanum lycopersicum L.) plant growth. Although the involvement of the tomato SlWRKY gene family in responses to saline‐alkali stress has been well established, the mechanism underlying resistance to saline‐alkali stress remains unclear. In this study, we investigated the role of SlWRKY81 in conferring saline‐alkali stress resistance by using overexpression and knockout tomato seedlings obtained via genetic modification. We demonstrated that SlWRKY81 improves the ability of tomato to withstand saline‐alkali stress by enhancing antioxidant capacity, root activity, and proline content while reducing malondialdehyde levels. Saline‐alkali stress induces an increase in jasmonic acid (JA) content in tomato seedlings, and the SlWRKY81 promoter responds to JA signaling, leading to an increase in SlWRKY81 expression. Furthermore, the interaction between SlJAZ1 and SlWRKY81 represses the expression of SlWRKY81. SlWRKY81 binds to W‐box motifs in the promoter regions of SlSPDS2 and SlNHX4, thereby positively regulating their expression. This regulation results in increased spermidine (Spd) content and enhanced potassium (K+) absorption and sodium (Na+) efflux, which contribute to the resistance of tomato to saline‐alkali stress. However, JA and SlJAZ1 exhibit antagonistic effects. Elevated JA content reduces the inhibitory effect of SlJAZ1 on SlWRKY81, leading to the release of additional SlWRKY81 protein and further augmenting the resistance of tomato to saline‐alkali stress. In summary, the modulation of Spd synthesis and Na+/K+ homeostasis mediated by the interaction between SlWRKY81 and SlJAZ1 represents a novel pathway underlying tomato response to saline‐alkali stress.

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“…Endogenous hormone levels play an important regulatory role in the process of plant flower bud differentiation [30]. ABA promotes flowering by inhibiting gibberellin biosynthesis, and indolepropionic acid (IPA) also delays flower senescence [31,32]. In Populus L., leaf morphology can be regulated by increasing the expression of IAA synthesis-related genes [33].…”

Section: Introductionmentioning

confidence: 99%

The Combined Analysis of the Transcriptome and Metabolome Revealed the Possible Mechanism of Flower Bud Formation in Amorphophallus bulbifer

Li,

Xu,

Qian

et al. 2024

Agronomy

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The flowering of Amorphophallus bulbifer (A. bulbifer) plays an important role in its reproduction. The flowers and leaves of A. bulbifer cannot grow at the same time. However, the physiological and molecular mechanisms involved in flower bud and leaf bud formation are still unclear. In this study, the flower buds and leaf buds of A. bulbifer in the early stage of growth were used as research materials, transcriptome and metabolome analyses were carried out, and the soluble sugar and starch contents of A. bulbifer corms were determined. Transcriptome analysis revealed 5542 differentially expressed genes (DEGs) between flower buds and leaf buds, 3107 of which were upregulated and 2435 of which were downregulated. Enrichment analysis of the KEGG pathway showed that these differential genes were enriched mainly in the plant hormone signal transduction, DNA replication and fatty acid elongation pathways. A total of 5296 significant differentially abundant metabolites were screened out by nontargeted metabolomics analysis. The differentially abundant metabolites were functionally classified in the HMDB, and 118 were successfully matched, including 17 that were highly expressed in flower buds. The differentially abundant metabolites in the flower buds were mainly enriched in pathways such as amino acid metabolism, isoquinoline alkaloid biosynthesis and pyrimidine metabolism. Targeted metabolomics analysis revealed that the contents of ABA, ZT and iPA in flower buds were significantly greater than those in leaf buds, while the opposite trend was observed for IAA. The analysis of soluble sugar and starch contents showed that the starch and soluble sugar contents in flower buds were significantly greater than those in leaf buds. The results of this study showed that flower bud development in A. bulbifer was regulated by amino acids, starch, ABA, ZT, iPA, IAA and other hormones. These findings could lead to valuable genetic resources for further study of A. bulbifer flowering and provide a deeper understanding of the molecular basis of A. bulbifer flowering.

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Tomato sucrose transporter SlSUT4 participates in flowering regulation by modulating gibberellin biosynthesis

Cited by 22 publications

References 81 publications

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

SlWRKY81 regulates Spd synthesis and Na⁺/K⁺ homeostasis through interaction with SlJAZ1 mediated JA pathway to improve tomato saline‐alkali resistance

The Combined Analysis of the Transcriptome and Metabolome Revealed the Possible Mechanism of Flower Bud Formation in Amorphophallus bulbifer

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Tomato sucrose transporter SlSUT4 participates in flowering regulation by modulating gibberellin biosynthesis

Cited by 22 publications

References 81 publications

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

Carbohydrates Are Associated with the Flowering Ability of Oncidesa Gower Ramsey ‘Honey Angel’

SlWRKY81 regulates Spd synthesis and Na+/K+ homeostasis through interaction with SlJAZ1 mediated JA pathway to improve tomato saline‐alkali resistance

The Combined Analysis of the Transcriptome and Metabolome Revealed the Possible Mechanism of Flower Bud Formation in Amorphophallus bulbifer

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SlWRKY81 regulates Spd synthesis and Na⁺/K⁺ homeostasis through interaction with SlJAZ1 mediated JA pathway to improve tomato saline‐alkali resistance