Tomato Yellow Leaf Curl China Virus Impairs Photosynthesis in the Infected Nicotiana benthamiana with βC1 as an Aggravating Factor

Farooq, Tahir; Liu, Dandan; Zhou, Xueping; Yang, Qiuying

doi:10.5423/ppj.oa.04.2019.0120

Cited by 11 publications

(5 citation statements)

References 28 publications

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“…In addition, pathways related to sugar metabolism were significantly down-regulated, which was also an important reason why plant growth was affected ( Figures 4B,C ). Therefore, tomato plants infected by ToCV were usually dwarf and small, which was consistent with previous studies ( Farooq et al, 2019 ; Zhao et al, 2020 ). We observed that photosynthesis-related genes were differentially regulated under different treatments ( Figure 6A ).…”

Section: Discussionsupporting

confidence: 92%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.

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Section: Discussionsupporting

confidence: 92%

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Hao

Huang

et al. 2021

Front. Microbiol.

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“…Utilizing cDNA arrays in conjunction with physiological assessments, a group of researchers investigated the impact of concurrent drought and heat shock stress on tobacco plants. The concurrent exposure to drought and heat shock conditions elicited stomatal closure (Farooq et al, 2019), inhibited photosynthetic processes (Vile et al, 2012), heightened respiratory activity, and elevated leaf temperature (Rizhsky et al, 2002). These findings are consistent with previous studies demonstrating the complex interactions between biotic and abiotic stresses on plant physiology (Atkinson and Urwin, 2012;Adhikari et al, 2020).…”

Section: Photosynthetic Rate Stomatal Conductance a Bsupporting

confidence: 90%

“…Notably, the combined effect of ToLCNDV infection and heat shock resulted in a substantially lower photosynthetic rate, indicating a synergistic negative impact of both stresses on tomato plants. In a similar study, tomato yellow leaf curl China virus (TYLCCNV) infection promptly led to a reduction in leaf surface area in Nicotiana benthamiana, subsequently resulting in a decrease in photosynthetic activity (Farooq et al, 2019). The heat-sensitive photosystem II activity experiences a significant reduction in the face of high-temperature stress.…”

Section: Photosynthetic Rate Stomatal Conductance a Bmentioning

confidence: 98%

Response of Tomato Plant to Abiotic (Heat Stress) and Biotic (Tomato Leaf Curl New Delhi Virus, TOLCNDV) Factors

Ali,

Khan,

Rao

2024

Int. J. Phytopathol.

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Tomato (Lycopersicon esculentum) is a vital fruit-bearing plant renowned for its nutritional advantages. Subjected to various environmental stresses, its yield is significantly impacted. Plants, confronting combined biotic and abiotic stresses, exhibit diverse responses through the evolution of cellular defense mechanisms. Sensitivity to extreme temperatures and pathogenic threats, such as the white fly-transmitted tomato leaf curl New Delhi virus (ToLCNDV), a Begomovirus, underscores the global susceptibility of tomato crops. This study aimed to assess tomato plant responses to the compounding stress of heat and agroinfiltrated ToLCNDV, analyzing four key physiological parameters viz., photosynthesis rate, stomatal conductance, transpiration rates, and Water use efficiency. Results indicated reduced photosynthesis rate of 5.1 ± 0.54 % mol CO2 m-2s-1, diminished stomatal conductance of 89.84±1.27% mmol.m-2s-1, and transpiration rates of 0.978 ± 0.148 %mmol.m-2s-1 as compared to control group as 8.56 ± 0.55 % mol CO2 m-2s-1, 189.08±19.13%mmol.m-2s-1and 2.586 ± 0.415 % mmol.m-2s-1respectively. Water use efficiency increased in double-stressed plants at 9.1 ± 0.96 % mmol CO2mol-1 H2O with respect to control 5.84 ± 0.48 % mmol CO2mol-1 H2O. The combined stressors manifested a net adverse impact, with neither ToLCNDV mitigating heat stress effects nor vice versa. Contrarily, their combined consequences were significantly amplified compared to individual stressors, emphasizing the intricate interplay of heat stress and ToLCNDV on tomato plants. This research marks a pioneering effort to uncover the physiological responses of a vulnerable tomato cultivar to the combined pressures of ToLCNDV and heat stress. Furthermore, it signifies a novel pathway towards the development of transgenic cultivars capable of effectively managing these stresses.

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“…In contrast, PEMV infection appeared to mainly reduce chlorophyll florescence without limiting leaf area, which is likely due to reduced photosynthetic efficiency by potentially interfering with photosynthetic machinery structures and functions at both early and late time points [59]. Other studies have similarly shown that virus infection can strongly impact photosynthetic efficiency by interacting with photosystem II regulation in plants [59,60]. By combining high-throughput phenotyping with other methods to explore plant chemical and physical defenses, our study provides a thorough view of how biotic stressors affect plant responses.…”

Section: Discussionmentioning

confidence: 89%

Diversity and Traits of Multiple Biotic Stressors Elicit Differential Defense Responses in Legumes

Basu,

Moroz,

Lee

et al. 2023

Agriculture

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In agroecosystems, plants frequently confront multiple biotic stressors, including herbivores and pathogens. The nature of these interactions plays a crucial role in mediating the activation of plant defense mechanisms. However, induction of plant chemical defenses has been more well studied than the induction of physical defenses. Here, we assessed the physical and chemical defense responses of pea (Pisum sativum) plants after exposure to three stressors: a vector herbivore (pea aphid, Acrythosiphon pisum), a non-vector herbivore (pea leaf weevil, Sitona lineatus), and a virus (Pea enation mosaic virus, PEMV). We used various histochemical staining techniques show that viruliferous A. pisum (transmitting PEMV) strongly induced callose deposition (aniline blue staining) and antioxidant-mediated defenses (DAB and NBT staining) in peas, primarily through accumulating reactive oxygen species (ROS). High-throughput phenotyping showed that viruliferous aphids reduced plant photosynthetic efficiency, but plants infected with PEMV had increased cell death (trypan blue staining). However, herbivory by aphids and weevils did not strongly induce defenses in peas, even though weevil feeding significantly reduced pea leaf area. These results show that not all herbivores induce strong defensive responses, and plant responses to vector species depends on their virus infection status. More broadly, our results indicate that variable stressors differentially regulate various plant responses through intricate chemical and physical defense pathways.

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Tomato Yellow Leaf Curl China Virus Impairs Photosynthesis in the Infected Nicotiana benthamiana with βC1 as an Aggravating Factor

Cited by 11 publications

References 28 publications

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Response of Tomato Plant to Abiotic (Heat Stress) and Biotic (Tomato Leaf Curl New Delhi Virus, TOLCNDV) Factors

Diversity and Traits of Multiple Biotic Stressors Elicit Differential Defense Responses in Legumes

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