Tomographic phase microscopy

Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran; Oh, Seungeun; Lue, Niyom; Dasari, Ramachandra R.; Feld, Michael S.

doi:10.1038/nmeth1078

Cited by 909 publications

(693 citation statements)

References 13 publications

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“…All phase projections were then processed digitally to create the 3D refractive‐index map of the cell by both the filtered back projection and the diffraction‐theory reconstruction algorithms 13, 30. In this reconstruction process, each projection is mapped to a surface in the 3D Fourier space, where the full rotation provided by DEP enables a full angular coverage of the Fourier space, in contrast to previous methods possessing limited angular range1, 2, 3, 4, 5, 6, 7 (see comparison in Figure 1). …”

Section: Resultsmentioning

confidence: 99%

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Rapid 3D Refractive‐Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation

et al. 2016

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A major challenge in the field of optical imaging of live cells is achieving rapid, 3D, and noninvasive imaging of isolated cells without labeling. If successful, many clinical procedures involving analysis and sorting of cells drawn from body fluids, including blood, can be significantly improved. A new label‐free tomographic interferometry approach is presented. This approach provides rapid capturing of the 3D refractive‐index distribution of single cells in suspension. The cells flow in a microfluidic channel, are trapped, and then rapidly rotated by dielectrophoretic forces in a noninvasive and precise manner. Interferometric projections of the rotated cell are acquired and processed into the cellular 3D refractive‐index map. Uniquely, this approach provides full (360°) coverage of the rotation angular range around any axis, and knowledge on the viewing angle. The experimental demonstrations presented include 3D, label‐free imaging of cancer cells and three types of white blood cells. This approach is expected to be useful for label‐free cell sorting, as well as for detection and monitoring of pathological conditions resulting in cellular morphology changes or occurrence of specific cell types in blood or other body fluids.

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Section: Resultsmentioning

confidence: 99%

“…To view the sample from multiple angles, one can rotate the illumination beam, while leaving the measured specimen stationary 1, 2, 3, 4, 5, 6, 7. This approach is noninvasive to the sample during data acquisition.…”

Section: Introductionmentioning

confidence: 99%

Rapid 3D Refractive‐Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation

et al. 2016

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“…3차원 굴절률 정보를 재구성하는 방식으로 이용하여 세포 내 부의 3차원 구조를 외부 염색 물질을 사용하지 않고 영상화 할 수 있으며, [25,26] 생리학적 또는 병리학적 변화에 따른 세 포 내부 구조의 변화를 연구하는 데도 이용되고 있다. [27,28] 합성 구경의 원리 …”

Section: 의 각도를 바꾸어가며 시편의 위상 정보를 얻어내어 시편의unclassified

Super-resolution Holographic Imaging Techniques Using a Synthetic Aperture

Jang¹,

Park²,

Kim³

et al. 2012

Phys. High Tech.

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“…Three-dimensional observation of weakly diffractive samples have, so far, been realized by varying the sample illumination with a fixed sample [3][4][5][6][7] or by rotating the sample using a fixed illumination [8][9][10][11][12]. In the sample illumination variation method, tomography is realized by varying the sample illumination angle in a range limited by the illumination system (usually a condenser) numerical aperture, drastically increasing the object spatial frequencies captured by this system in comparison to a conventional holographic transmission microscope, which uses only one direction of illumination.…”

Section: Introductionmentioning

confidence: 99%

Diffraction microtomography with sample rotation: influence of a missing apple core in the recorded frequency space

Vertu¹,

Delaunay²,

Yamada³

et al. 2009

Open Physics

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Abstract:Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives morphological information only, diffraction microtomography makes it possible to obtain the complex optical refractive index of the observed sample by mapping a three-dimensional support in the spatial frequency domain. The technique can be implemented in two configurations, namely, by varying the sample illumination with a fixed sample or by rotating the sample using a fixed illumination. In the literature, only the former method was described in detail. In this report, we precisely derive the three-dimensional frequency support that can be mapped by the sample rotation configuration. We found that, within the first-order Born approximation, the volume of the frequency domain that can be mapped exhibits a missing part, the shape of which resembles that of an apple core. The projection of the diffracted waves in the frequency space onto the set of sphere caps covered by the sample rotation does not allow for a complete mapping of the frequency along the axis of rotation due to the finite radius of the sphere caps. We present simulations of the effects of this missing information on the reconstruction of ideal objects. ; 42.30.-d; 42.40.-i; 42.90.+m PACS (2008): 42.

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Tomographic phase microscopy

Cited by 909 publications

References 13 publications

Rapid 3D Refractive‐Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation

Rapid 3D Refractive‐Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation

Super-resolution Holographic Imaging Techniques Using a Synthetic Aperture

Diffraction microtomography with sample rotation: influence of a missing apple core in the recorded frequency space

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