Tongue microbiome in children with autism spectrum disorder

Halboub, Esam; Homeida, Husham E.; Basode, Vinod Kumar; Ghzwani, Ahmad Hassn; Zain, Khalid Ammash; Baraniya, Divyashri; Chen, Tsute; Al-Hebshi, Nezar N.

doi:10.1080/20002297.2021.1936434

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…In the human oral cavity, Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, and Actinobacteria were the dominant phyla that are consistent with previous oral microbiota studies (Stahringer et al, 2012;Moon and Lee, 2016;van der Meulen et al, 2018;Abdulhaq et al, 2021). Firmicutes was the most abundant phylum in the saliva, although its mean relative abundance was significantly lower than that in the buccal mucosa (p < 0.0001).…”

Section: Differences Of Salivary and Buccal Mucosal Microbiotasupporting

confidence: 87%

Comparative Evaluation of the Salivary and Buccal Mucosal Microbiota by 16S rRNA Sequencing for Forensic Investigations

Wang

Song

et al. 2022

Front. Microbiol.

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The human microbiome has emerged as a new potential biomarker for forensic investigations with the development of high-throughput sequencing and bioinformatic analysis during the last decade. The oral cavity has many different microbial habitats, with each habit colonized by specific and individualized microbiota. As saliva and buccal mucosa are common biological evidence in forensic science, understanding the differences of microbial communities between the two is important for forensic original identification. Moreover, the oral microbiota is individualized, whereas there are few studies on the application of forensic personal identification that need to be supplemented. In this study, Streptococcus was the most abundant genus, with an average relative abundance of 49.61% in the buccal mucosa, while in the saliva, Streptococcus, Veillonella, and Neisseria had similar proportions (20%, 15%, 16%) and were the dominant genera. The α and β diversity displayed a significant distinctness between the saliva and buccal mucosal groups. The community assembly mechanism stated that the deterministic process played a more significant effect in shaping the salivary bacterial community assembly than buccal mucosa, which explained the microbial differences. Of the test samples, 93.3% can be correctly classified with the random forest model based on the microbial differences. Targeting the low-abundance bacteria at the species level, 52% of experimental participants could be discriminated by using the observed unique bacterial species. In conclusion, the salivary bacterial community composition differed from that of the buccal mucosa and showed high richness and diversity. With the random forest model, the microbiota of saliva and buccal mucosa can be classified, which can be used in identifying the source of oral biological trace. Furthermore, each individual has a unique bacterial community pattern, and the presence or absence of unique bacteria and differences in the composition of the core oral microbiota are the key points for forensic personal discrimination that supplement the study of oral microbial application to forensic personal discrimination. Whether for original identification or personal discrimination, the oral microbiome has great potential for application.

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Section: Differences Of Salivary and Buccal Mucosal Microbiotasupporting

confidence: 87%

Comparative Evaluation of the Salivary and Buccal Mucosal Microbiota by 16S rRNA Sequencing for Forensic Investigations

Wang

Song

et al. 2022

Front. Microbiol.

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“…Microbial alterations were observed, including decreased rates of the genera Prevotella and Alloprevotella in saliva, but also a reduction of the family Prevotellaceae co-occurrence network in dental plaque of ASD children [ 224 ]. Recently, the tongue microbiome was examined to assess whether there could be a link between the bacterial composition on the tongue surface and ASD [ 225 ]. The genus Prevotella and the species P. melaninogenica were among the most abundant findings in studied children; however, no significant differences were detected between ASD and control children.…”

Section: Potential Involvement Of Oral Prevotella ...mentioning

confidence: 99%

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

Könönen

Fteita

Gürsoy

et al. 2022

Journal of Oral Microbiology

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Oral Prevotella are known as anaerobic commensals on oral mucosae and in dental plaques from early life onwards, including pigmented P. melaninogenica, P. nigrescens, and P. pallens and non-pigmented Prevotella species. Many Prevotella species contribute to oral inflammatory processes, being frequent findings in dysbiotic biofilms of periodontal diseases ( P. intermedia, P. nigrescens ), cariotic lesions ( P. denticola, Alloprevotella (formerly Prevotella ) tannerae ), endodontic infections ( P. baroniae, P. oris, P. multisaccharivorax ), and other clinically relevant oral conditions. Over the years, several novel species have been recovered from the oral cavity without knowledge of their clinical relevance. Within this wide genus, virulence properties and other characteristics like biofilm formation seemingly vary in a species- and strain-dependent manner, as shown for the P. intermedia group organisms ( P. aurantiaca, P. intermedia, P. nigrescens , and P. pallens ). Oral Prevotella species are identified in various non-oral infections and chronic pathological conditions. Here, we have updated the knowledge of the genus Prevotella and the role of Prevotella species as residents and infectious agents of the oral cavity, as well as their detection in non-oral infections, but also gathered information on their potential link to cancers of the head and neck, and other systemic disorders.

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“…Thus, the present data confirm a high similarity between young patients with CKD and healthy individuals. A direct comparison of our patient population with a historical control group of healthy children [ 26 ] shows high similarity at the genus level (see Supplementary Table 4 ). Differences at the species level cannot be meaningfully evaluated because different databases were used in the different trials.…”

Section: Discussionmentioning

confidence: 96%

The tongue microbiome of young patients with chronic kidney disease and their healthy mothers

Hoefer,

Weber,

Barbe

et al. 2024

Clin Oral Invest

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Objectives Oral microbiome plays a crucial role in the incidence and development of oral diseases. An altered intestinal microbiome has been reported in adults with chronic kidney disease (CKD). This study aimed to characterize the tongue microbiome of young patients with CKD compared to their healthy mothers to identify the influence of CKD-associated factors on resilient tongue ecosystem. Material and methods Thirty patients with CKD (mean age, 14.2 years; 16 males and 14 females) and generalized gingivitis were included in the study. Swabs of the posterior tongue were collected from the patients and 21 mothers (mean age 40.8 years). Next-generation sequencing of 16S rDNA genes was employed to quantitatively characterize microbial communities. Results The bacterial communities were similar in terms of richness and diversity between patients and mothers (p > 0.05). In patients with CKD, 5 core phyla, 20 core genera, and 12 core species were identified. Conclusions The tongue microbiome of the study participants showed no relevant CKD-associated differences compared to their mothers and appears to be a highly preserved niche in the oral cavity. Differences observed in the abundance of individual species in this study could be attributed to the age rather than CKD, even after a mean disease duration of 11 years. Clinical relevance CKD and its associated metabolic changes appear to have no detectable impact on the resilient tongue microbiome observed in young patients.

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Tongue microbiome in children with autism spectrum disorder

Cited by 11 publications

References 51 publications

Comparative Evaluation of the Salivary and Buccal Mucosal Microbiota by 16S rRNA Sequencing for Forensic Investigations

Comparative Evaluation of the Salivary and Buccal Mucosal Microbiota by 16S rRNA Sequencing for Forensic Investigations

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

The tongue microbiome of young patients with chronic kidney disease and their healthy mothers

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