Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis

He, Yani; Peng, Jia-Shi; Cai, Yao; Liu, De Fen; Yuan, Guohui; Yi, Hong; Gong, Ji-Ming

doi:10.1038/s41598-017-06744-5

Cited by 49 publications

(52 citation statements)

References 56 publications

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“…4a-c ), indicating that elevated expression of OsNPF7.2 contributes to nitrate allocation between roots and shoots. Recently, three NRT1/NPF family members (NPF5.11, NPF5.12 and NPF5.16) in Arabidopsis were reported to be localized at vacuolar membrane and to play a possible role in modulating nitrate allocation between roots and shoots (He et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield

Wang

Lü

Nie

et al. 2018

Rice

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BackgroundRice tiller number is one of the most important factors that determine grain yield, while nitrogen is essential for the crop growth and development, especially for tiller formation. Genes involved in nitrogen use efficiency processes have been identified in the previous studies, however, only a small number of these genes have been found to improve grain yield by promoting tillering.ResultsWe constructed over-expression (OX) lines and RNA-interference (Ri) lines, and selected a mutant of OsNPF7.2, a low-affinity nitrate transporter. Our analyses showed that rice tiller number and grain yield were significantly increased in OX lines, whereas Ri lines and mutant osnpf7.2 had fewer tiller number and lower grain yield. Under different nitrate concentrations, tiller buds grew faster in OX lines than in WT, but they grew slower in Ri lines and mutant osnpf7.2. These results indicated that altered expression of OsNPF7.2 plays a significant role in the control of tiller bud growth and regulation of tillering. Elevated expression of OsNPF7.2 also improved root length, root number, fresh weight, and dry weight. However, reduced expression of OsNPF7.2 had the opposite result on these characters. OsNPF7.2 OX lines showed more significantly enhanced influx of nitrate and had a higher nitrate concentration than WT. The levels of gene transcripts related to cytokinin pathway and cell cycle in tiller bud, and cytokinins concentration in tiller basal portion were higher in OX lines than that in WT, suggesting that altered expression of OsNPF7.2 controlled tiller bud growth and root development by regulating cytokinins content and cell cycle in plant cells. Altered expression of OsNPF7.2 also was responsible for the change in expression of the genes involved in strigolactone pathway, such as D27, D17, D10, Os900, Os1400, D14, D3, and OsFC1.ConclusionOur results suggested that OsNPF7.2 is a positive regulator of nitrate influx and concentration, and that it also regulates cell division in tiller bud and alters expression of genes involved in cytokinin and strigolactone pathways, resulting in the control over rice tiller number. Since elevated expression of OsNPF7.2 is capable of improving rice grain yield, this gene might be applied to high-yield rice breeding.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0205-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield

Wang

Lü

Nie

et al. 2018

Rice

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“…Finally, the NPF5 subfamily comprises 16 members but remains very poorly studied. It includes one member of the PTR group, NPF5.2 (described in the NPF8 Subfamily section below), the tonoplastic NPF5.11, NPF5.12, and NPF5.16, for which a role in nitrate transport from vacuoles to modulate nitrogen allocation in the root-to-shoot system was proposed (He et al, 2017), and NPF5.5, whose loss-offunction mutants show reduced nitrogen accumulation (Lé ran et al, 2015).…”

Section: The Heterogeneous Npf2 Subfamilymentioning

confidence: 99%

More Transporters, More Substrates: The Arabidopsis Major Facilitator Superfamily Revisited

et al. 2019

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The Major Facilitator Superfamily (MFS) is ubiquitous in living organisms and represents the largest group of secondary active membrane transporters. In plants, significant research efforts have focused on the role of specific families within the MFS, particularly those transporting macronutrients (C, N, and P) that constitute the vast majority of the members of this superfamily. Other MFS families remain less explored, although a plethora of additional substrates and physiological functions have been uncovered. Nevertheless, the lack of a systematic approach to analyzing the MFS as a whole has obscured the high diversity and versatility of these transporters. Here, we present a phylogenetic analysis of all annotated MFS domaincontaining proteins encoded in the Arabidopsis thaliana genome and propose that this superfamily of transporters consists of 218 members, clustered in 22 families. In reviewing the available information regarding the diversity in biological functions and substrates of Arabidopsis MFS members, we provide arguments for intensified research on these membrane transporters to unveil the breadth of their physiological relevance, disclose the molecular mechanisms underlying their mode of action, and explore their biotechnological potential.

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“…Currently, AtNPF2.9 is the only gene that has been confirmed to play a role in the loading of NO 3 − into root phloem and negatively regulates the root to shoot transport of NO 3 − (Fan et al, 2009;Wang and Tsay, 2011). It has also been established that AtNPF5.11, AtNPF5.12, and AtNPF5.16 play roles in the uptake of NO 3 − from the vacuole to the cytoplasm in Arabidopsis to regulate the shoot:root ratio, and in a triple mutant for these genes, larger amounts of NO 3 − were found to be translocated to the shoots (He et al, 2017). In Arabidopsis, AtNPF2.9, AtNPF7.2, and AtNPF7.3 are the main NO 3 − transporters contributing to the regulation of the long-distance transport and redistribution of NO 3 − from roots to shoots ( Figure 1C).…”

Section: Atnpf72 Is Expressed In Xylem Parenchyma Cells and Affects mentioning

confidence: 99%

Reducing the Nitrate Content in Vegetables Through Joint Regulation of Short-Distance Distribution and Long-Distance Transport

Liang

Zhang

2020

Front. Plant Sci.

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As an important nitrogen source, nitrate (NO 3 −) absorbed by plants is carried throughout the plant via short-distance distribution (cytoplasm to vacuole) and long-distance transportation (root to shoot), the two pathways that jointly regulate the content of NO 3 − in plants. NO 3 − accumulation within the vacuole depends on the activities of both tonoplast proton pumps and chloride channel (CLC) proteins, and less NO 3 − is stored in vacuoles when the activities of these proteins are reduced. The ratio of the distribution of NO 3 − in the cytoplasm and vacuole affects the long-distance transport of NO 3 − , which is regulated by the proteins NPF7.3 and NPF7.2 that play opposite but complementary roles. NPF7.3 is responsible for loading NO 3 − from the root cytoplasm into the xylem, whereas NPF7.2 regulates the unloading of NO 3 − from the xylem, thereby facilitating the long-distance transport of NO 3 − through the roots to the shoots. Vegetables, valued for their nutrient content, are consumed in large quantities; however, a high content of NO 3 − can detrimentally affect the quality of these plants. NO 3 − that is not assimilated and utilized in plant tissues is converted via enzyme-catalyzed reactions to nitrite (NO 2 −), which is toxic to plants and harmful to human health. In this review, we describe the mechanisms underlying NO 3 − distribution and transport in plants, a knowledge of which will contribute to breeding leafy vegetables with lower NO 3 − contents and thus be of considerable significance from the perspectives of environmental protection and food safety.

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Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis

Cited by 49 publications

References 56 publications

Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield

Rice nitrate transporter OsNPF7.2 positively regulates tiller number and grain yield

More Transporters, More Substrates: The Arabidopsis Major Facilitator Superfamily Revisited

Reducing the Nitrate Content in Vegetables Through Joint Regulation of Short-Distance Distribution and Long-Distance Transport

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