Too smart to fail—how viruses exploit the complexity of host cells during entry

Greber, Urs F.; Cosset, François‐Loïc

doi:10.1016/j.coviro.2011.05.011

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…HAdV-B3 is a representative of the species B types that utilize CD46 and/or desmoglein 2 (DSG2) as attachment receptors [39, 43, 44]. The underlying complexity of host factors controlling virus entry and infection is, however, incompletely understood [45]. Accurate analyses of global protein expression patterns in infected cells can contribute to a better understanding of essential virus-host interactions.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections

Trinh

Grossmann

Gehrig

et al. 2013

International Journal of Proteomics

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Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQ-labeled samples, while Progenesis LC-MS and ProgenesisF-T2PQ/T3PQ were employed for label-free analyses. R 2 correlation coefficients correlated well between two software packages applied to the same datasets with values between 0.48 and 0.78 for iTRAQ-label-based quantitations and 0.5 and 0.86 for label-free quantitations. Analyses of label-free samples showed higher levels of protein up- or downregulation in comparison to iTRAQ-labeled samples. The concentration differences were further evaluated by Western blotting for four downregulated proteins. These data suggested that the label-free method was more accurate than the iTRAQ method.

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Section: Introductionmentioning

confidence: 99%

iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections

Trinh

Grossmann

Gehrig

et al. 2013

International Journal of Proteomics

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show abstract

“…It is thought that four general biological alterations accompany the early-to-late endosomal transition: acidification of the endosomal lumen, formation of luminal vesicles, the switch of Rab GTPases, and microtubule-mediated transport between the organelles (Greber & Cosset, 2011). Some viruses escape to the cytoplasm under the effects of the acidic environment (pH 6.5 to 6.0) of the early endosome.…”

Section: Transition To the Late Endosomementioning

confidence: 99%

“…These are late penetrating viruses, in the sense that they are pH-dependent and persist longer through the endocytic transport system than earlier penetrating viruses (Greber, 2011). The rugged, mature, extracellular virion undergoes multistep conformational changes that are time-and cell compartment-dependent.…”

Section: Introductionmentioning

confidence: 99%