Tools for biotechnological production of useful phytochemicals from adventitious root cultures

Murthy, Hosakatte Niranjana; Dandin, Vijayalaxmi S.; Paek, Kee-Yoeup

doi:10.1007/s11101-014-9391-z

Cited by 75 publications

(32 citation statements)

References 108 publications

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“…Airlift bioreactors appear to be ideal for plant cell and organ cultures by efficiently controlling the culture environment, foam generation, shear stress, and oxygen supply [78,83,84]. They are suitable for the cultivation of cell, hairy root, adventitious root and embryo suspension cultures of various medicinal plants [32,78,84,85], and are also applied in MJ treatment to reduce the inhibition of root biomass by two 2-stage cultures. The first step involves the optimization of root growth in bioreactors without elicitor and then concentration and time of elicitor application will have to be standardized in the second step.…”

Section: Application Of Mj For Enhancement Of Secondary Metabolites Imentioning

confidence: 99%

“…Moreover, total productivity was also increased in both plants with 1.92-fold and 1.12-fold compared with non-treated roots. In addition, the treatment of MJ for improving bioactive compounds is also successfully applied in pilot-scale bioreactors up to 10,000 L [3,[84][85][86]. A pilot-scale (500-L) bioreactor cultures were established in adventitious root culture of Echinacea angustifolia [87].…”

Section: Application Of Mj For Enhancement Of Secondary Metabolites Imentioning

confidence: 99%

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Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Murthy

Park

2020

IJMS

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181

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Recently, plant secondary metabolites are considered as important sources of pharmaceuticals, food additives, flavours, cosmetics, and other industrial products. The accumulation of secondary metabolites in plant cell and organ cultures often occurs when cultures are subjected to varied kinds of stresses including elicitors or signal molecules. Application of exogenous jasmonic acid (JA) and methyl jasmonate (MJ) is responsible for the induction of reactive oxygen species (ROS) and subsequent defence mechanisms in cultured cells and organs. It is also responsible for the induction of signal transduction, the expression of many defence genes followed by the accumulation of secondary metabolites. In this review, the application of exogenous MJ elicitation strategies on the induction of defence mechanism and secondary metabolite accumulation in cell and organ cultures is introduced and discussed. The information presented here is useful for efficient large-scale production of plant secondary metabolites by the plant cell and organ cultures.

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Section: Application Of Mj For Enhancement Of Secondary Metabolites Imentioning

confidence: 99%

Section: Application Of Mj For Enhancement Of Secondary Metabolites Imentioning

confidence: 99%

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Murthy

Park

2020

IJMS

Self Cite

181

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“…Hence, roots have been studied to induce and culture in vitro systems, such as adventitious root cultures, that are not infected with Agrobacterium rhizogenes 2 . Adventitious roots are natural and genetically well-established organs, in contrast to cultured plant cell suspensions, and they are useful for biosynthetic investigation as well as for biotechnological applications 5 6 . Cytotoxic triterpenoids that accumulate in adventitious roots are of great interest due to their extensive range of biological activity, especially in their potential effects against human tumor cell lines 7 8 .…”

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confidence: 99%

Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging

Pina

Silva

Teixeira

et al. 2016

Sci Rep

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Biosynthetic investigation of quinonemethide triterpenoid 22β-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using 13C-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22β-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes.

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“…As an alternative to field‐cultivated P. ginseng , in vitro tissue culture include cells, callus, hairy roots, and adventitious roots have been applied to produce ginseng. Among them, adventitious root have higher production compared to suspension cells and more natural for hairy roots because they contain no bacterial DNA sequences (An et al, ; Lee, Choi, et al, ; Liu et al, ; Murthy, Dandin, & Paek, ; Wang, Man, Gao, Zhang, & Huang, ). Previous studies have showed that the ginsenoside contents of P. ginseng adventitious root were lower than those cultivated plants, such as monomeric ginsenoside Rg 1 , Rb 1 , Rg 2 , Rc, Rb 2 , Rd, Re, and total saponin (Huang et al, ; Li et al, ; Liu et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Among them, adventitious root have higher production compared to suspension cells and more natural for hairy roots because they contain no bacterial DNA sequences Murthy, Dandin, & Paek, 2014;Wang, Man, Gao, Zhang, & Huang, 2013). Previous studies have showed that the ginsenoside contents of P. ginseng adventitious root were lower than those cultivated plants, such as monomeric ginsenoside Rg 1 , Rb 1 , Rg 2 , Rc, Rb 2 , Rd, Re, and total saponin (Huang et al, 2010;Li et al, 2019;Liu et al, 2014;Wang et al, 2013).…”

mentioning

confidence: 99%

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

et al. 2019

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In the study, six adventitious root lines of Panax ginseng have been successfully established. HPLC‐ESI‐MS analysis showed that 20 ginsenosides were identified in root lines, notoginsenoside Fa and notoginsenoside R2 were not found in AR lines. In AR lines, the highest accumulation of total ginsenosides was obtained in five‐year main AR (24.87 mg/g). Principal component analysis classified root lines into three groups. Five‐year ginseng was mostly similar with five‐year main AR, five‐year rootlet AR, and four‐year rootlet AR in ginsenosides composition of group 1. Besides, gene expressions were consistent with the production of total ginsenosides, and correlation analysis revealed that total ginsenosides biosynthesis was significantly positively correlated with the gene expression of dammarenediol synthase. Five‐year rootlet AR showed the highest activity on ferric‐reducing antioxidant power test among samples. It provides a scientific evidence for the further exploitation and large‐scale production of P. ginseng. Practical applications This study provides valuable information for the commercial scale culture of ginseng adventitious roots. This report combines morphology, ginsenoside composition and content, gene expression, and ferric‐reducing antioxidant power test to evaluate the quality of P. ginseng adventitious root, and combined with principal component analysis to screen out the high yield and stable ginseng adventitious roots. It would be profitable to use adventitious root culture of P. ginseng instead of field cultivation.

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Tools for biotechnological production of useful phytochemicals from adventitious root cultures

Cited by 75 publications

References 108 publications

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

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