Objectives: To evaluate different desensitizing and anti-erosive toothpastes on dentin surface loss (SL) and the number of open dentin tubules (ODT).Methods: The 10 groups (n=10) were: 1. Distilled water - negative control (N), 2. Colgate Total 12 - positive control (CT); 3. Bianco Pro Clinical (BP); 4. Bianco Advanced Repair (BA), 5. Elmex Sensitive (ES), 6. Elmex Anticaries (EA), 7. Elmex Erosion (EE); 8. Sensodyne Rapid Relief (SRR); 9. Sensodyne Repair&Protect (SRP), and 10. Colgate Pro-Relief (CP). The cycling consisting of immersion in 0.3 % citric acid (5 min) and artificial saliva (60 min), 4×/day; 5 days. Toothbrushing was performed 2x/day (45 strokes). The SL was determined using an optical profilometer. All toothpastes had their pH evaluated. The specimens were also analyzed using a scanning electron microscope (SEM) to verify the ODT, and energy dispersive spectrometry (EDS). Results: EA showed the lowest SL (p < 0.05), but not differing from EE, SRP, BPC, SRR, ES, and BA. Only the groups EA, EE and SRP was different statistically from negative and positive control . The SEM results revealed that SRP had the lowest ODT (p = 0.004). Negative control, ES, and BP showed no difference in ODT from initial condition (p > 0.005). There was a weak positive correlation between SL and ODT and SL with pH, and a negative correlation was observed between ODT and pH (all p > 0.05). Conclusion: SRP, EE, and EA decreased SL; only SRP decreased the number of ODT Clinical Significance: Some toothpastes may be more suitable for preventing only erosive tooth wear such as Elmex Erosion, Elmex Anti Caries and Sensodyne Repair and Protect, and the latter may be indicated for tubular occlusion as well. In addition, as Sensodyne Repair and Protect offers interesting results for both situations, can be indicated for patients with both conditions in the oral environment: erosive tooth wear and dentin hypersensitivity.