Topical Activity of Bendazac on Experimental Burns

Abstract

Bendazac or 1-benzyl-indazole 3-oxyacetic acid is a topical antinflammatory drug which in previous experiments was shown to preferentially inhibit inflammatory processes progressing towards necrosis (1). This activity has been temptatively related to a pre vention of protein denaturation (2-4).The aim of these experiments was to study effects of bendazac and hydrocortisone on heat-induced burns and on acetic acid-induced erythema. Two differently formulated creams have been investigated, in an attempt to find out the most suitable one for topical uses. METHODSCFI mice and Wistar rats of both sexes, weighing 20-30 and 130-170 g respectively, were used. The skin areas to be treated were clipped with an electric shaver. When not otherwise indicated, animals were kept under urethane anesthesia (1.7 g/kg i.p. in rats and 1.5 g/kg i.p. in mice) throughout the whole experiment.Burns were produced in rats by putting on the abdomen for 30 seconds a test tube (weighing 50 g and with a diameter of about 8 mm) in which water was circulated at a temperature of 70°C. In each animal two burns were produced, one on the upper third and the other on the lower third of the abdomen. Two minutes later, the burnt areas were rubbed gently with 2 ml of the creams under investigation. One hour later, the ani mals were intravenously injected with 1 ml of a water solution containing 0.5% Evans Blue and 0.9% NaC1. Two hours after application of the creams, animals were killed by a blow on the head and the burnt skin was stretched and inserted between two glass slides. The intensity of blueing, resulting from accumulation of Evans Blue in the injured skin, was estimated by a Joyce Loebl mod. II photodensitometer devised for automatic read ing of electrophoretic tracings.Acetic acid erythema was studied in mice. A disc of filter paper, 4 mm in diameter, was soaked with glacial acetic acid and applied on the back of animals taking care that the paper adhered to the skin. After 15 seconds the disc was removed, the excess of acetic acid was wiped and a minute later 0.5 ml of the creams were applied locally. One hour later, the animals were intravenously injected with 0.5 ml of a water solution containing 0.1 % Evans Blue and 0.9 % NaC1. After 30 minutes animals were killed by a blow on

Topical Anti-Inflammatory Effects of Af 2071: A Comparative Study With Bendazac and Corticosteroids, Both Alone and Associated

“…With acetic acid-induced erythema we referred to a method previously used in mice (3). Throughout the entire experiment the animals were kept under urethane anaesthesia (1.5 g/kg i.p.).…”

Animal models for the study of atypical anti-inflammatory agents

“…Nevertheless, it was found to inhibit the necrotic lesions ensuing or accompanying some inflammatory processes, such as heat burns and urea necrosis following topical or systemic administration [59,60,61].…”