Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model

S, Pereira Ddos; Mh, Lima-Ribeiro; Santos-Oliveira, Ralph; L, Cavalcanti Cde; Nt, de Pontes-Filho; Lc, Coelho; Carneiro-Leão, Ana Maria dos Anjos; Mt, Correia

doi:10.1155/2012/184538

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…On the other hand, development of histological tissues obtained in this study were consistent with observed by Pereira et al [ 28 ], where the reepithelialization time was lower for animals treated with isolectin hydrogel, starting around the burn edge on the 14th day, and the injuries have been completely reepithelialized in 35 days.…”

Section: Discussionsupporting

confidence: 92%

Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents

et al. 2015

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BackgroundEvery year thousands of people are victims of burns, mainly scald burns. Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care. As in Brazil Casearia sylvestris Sw., popularly known as guaçatonga is widely used for its analgesic, antiseptic and anti-inflammatory activities, this study sought to evaluate the effects of its hydroalcoholic extract in healing process of burns injuries.MethodsThe obtained extract was validated applying a thin layer chromatography and sophisticated validation method using Bothrops jararacussu snake venom that is necrotic and inflammatory, and by which guaçatonga extract was able to neutralize the irreversible neuromuscular blockade induced by the venom. After induction of the scald injury, the animals were treated daily with saline solution spray; spray containing extract; biofilm; or biofilm impregnated with extract.ResultsSignificant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.ConclusionThe anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

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Section: Discussionsupporting

confidence: 92%

Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents

et al. 2015

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“…In present study, we observed that upon completion of wound healing few scar tissues remained in all treated rats. Scar formation in all kinds of wound healing are normal [52] and exist even after complete healing. However, the AM treated experimental group had the lowest scar formation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

Rahman

Islam

Rana

et al. 2019

BMC Complement Altern Med

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Background Skin burn wound is a notable medical burden worldwide. Rapid and effective treatment of burnt skin is vital to fasten wound closure and healing properly. Amniotic graft and Aloe vera are widely used as wound managing biomaterials. Sophisticated processing, high cost, availability, and the requirement of medics for transplantation limit the application of amnion grafts. We aim to prepare a novel gel from amnion combined with the Aloe vera extract for burn wound healing which overcome the limitations of graft. Methods Two percent human amniotic membrane (AM), Aloe vera (AV) and AM+AV gels were prepared. In vitro cytotoxicity, biocompatibility, cell attachment, proliferation, wound healing scratch assays were performed in presence of the distinct gels. After skin irritation study, second-degree burns were induced on dorsal region of Wistar rats; and gels were applied to observe the healing potential in vivo. Besides, macroscopical measurement of wound contraction and re-epithelialization; gel treated skin was histologically investigated by Hematoxylin and eosin (H&E) staining. Finally, quantitative assessment of angiogenesis, inflammation, and epithelialization was done. Results The gels were tested to be non-cytotoxic to nauplii and compatible with human blood and skin cells. Media containing 500 μg/mL AM+AV gel were observed to promote HaCaT and HFF1 cells attachment and proliferation. In vitro scratch assay demonstrated that AM+AV significantly accelerated wound closure through migration of HaCaT cells. No erythema and edema were observed in skin irritation experiments confirming the applicability of the gels. AV and AM+AV groups showed significantly accelerated wound closure through re-epithelialization and wound contraction with P < 0.01. Macroscopically, AM and AM+AV treated wound recovery rates were 87 and 90% respectively with P < 0.05. Histology analysis revealed significant epitheliazation and angiogenesis in AM+AV treated rats compared to control ( P < 0.05). AM+AV treated wounds had thicker regenerated epidermis, increased number of blood vessels, and greater number of proliferating keratinocytes within the epidermis. Conclusion We demonstrated that a gel consisting of a combination of amnion and Aloe vera extract has high efficacy as a burn wound healing product. Amniotic membrane combined with the carrier Aloe vera in gel format is easy to produce and to apply.

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“…nilotica extract gel accelerates uninfected [5] or infected burn wound healing. As reported by Kumar et al (2006) [14] and Peraira et al (2012) [15], burn wounds were usually infected by bacteria gram positive like S. aureus and this infection delays wound healing. The significant burn wound healing effect of A. nilotica extract observed in the infected group associates with delay wound healing observed after wound infection by staphylococcus aureus in controls indicating that A. nilotica pods extract are responsible for an effective antimicrobial activity.…”

Section: Discussionmentioning

confidence: 86%

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

Metowogo

Darré

Daubeuf

et al. 2015

J. Pharm. Nutr. Sci.

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Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect. Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal's burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against-2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked. In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 10 9 CFU/mL of S. aureus.

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Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model

Cited by 12 publications

References 36 publications

Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents

Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents

Characterization of burn wound healing gel prepared from human amniotic membrane and Aloe vera extract

Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns

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