Topical Application of Antisense Oligonucleotide-Loaded Chitosan Nanoparticles to Rats

Özbaş-Turan, Suna; Akbuğa, Jülide; Sezer, Ali Demir

doi:10.1089/oli.2009.0222

Cited by 29 publications

(16 citation statements)

References 24 publications

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“…The particle sizes of C3F1, C4F1, and C5F1 NPs were approximately 300 nm, 330 nm, and 270 nm, respectively, at pH 6.0 to pH 7.4 (Table 3). Nanosized particles have been reported to have an obstinate residency in the lungs [38] and an ability to evade phagocytosis and mucociliary clearance [39,40]. Moreover, nanoparticles exhibiting an approximate diameter of 250 nm are likely able to diffuse through lung mucus more easily than larger NP formulations can [41].…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

Huang

2014

Marine Drugs

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In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230–250 nm, as demonstrated by TEM. These CS/F NPs maintained compactness and stability for 25 day in phosphate-buffered saline (pH 6.0–7.4). The CS/F NPs exhibited highly potent antioxidant effects by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing the concentration of intracellular reactive oxygen species (ROS) and superoxide anion (O2−) in stimulated macrophages. The DPPH scavenging effect of CS/F NPs primarily derives from fucoidan. Furthermore, these CS/F NPs activated no host immune cells into inflammation-mediated cytotoxic conditions induced by IL-6 production and NO generation. The MTT cell viability assay revealed an absence of toxicity in A549 cells after exposure to the formulations containing 0.375 mg NPs/mL to 3 mg NPs/mL. Gentamicin (GM), an antibiotic, was used as a model drug for an in vitro releasing test. The CS/F NPs controlled the release of GM for up to 72 h, with 99% of release. The antioxidant CS/F NPs prepared in this study could thus be effective in delivering antibiotics to the lungs, particularly for airway inflammatory diseases.

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Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

Huang

2014

Marine Drugs

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“…The high permeability of ASOND into skin layer was noted to be probably due to the effect of chitosan on tight junctions and an increase in permeability. 31 Manchanda et al have described that controlled-size chitosan nanoparticles produced using reverse micellar system is an efficient biocompatible ASOND delivery system with high cellular viability compared with lipofectamine. 32 It has also been shown that chitosan nanoparticles protect the ASOND molecule during preparation and treatment.…”

Section: Resultsmentioning

confidence: 99%

Thiolated chitosan nanoparticles as a delivery system for antisense therapy: evaluation against EGFR in T47D breast cancer cells

Talaei

Azizi²,

Dinarvand³

et al. 2011

IJN

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Thiolated chitosan has high transfection and mucoadhesive properties. We investigated the potential of two recently synthesized polymers: NAC-C (N-acetyl cysteine-chitosan) and NAP-C (N-acetyl penicillamine-chitosan) in anticancer drug delivery targeting epidermal growth factor receptor (EGFR). Doxorubicin (DOX) and antisense oligonucleotide (ASOND)-loaded polymer nanoparticles were prepared in water by a gelation process. Particle characterization, drug loading, and drug release were evaluated. To verify drug delivery efficiency in vitro experiments on a breast cancer cell line (T47D) were performed. EGFR gene and protein expression was analyzed by real time quantitative polymerase chain reaction and Western blotting, respectively. A loading percentage of 63% ± 5% for ASOND and 70% ± 5% for DOX was achieved. Drug release data after 15 hours showed that ASOND and DOX were completely released from chitosan-based particles while a lower and more sustained release of only 22% ± 8% was measured for thiolated particles. In a cytosol simulated release medium/reducing environment, such as found intracellularly, polymer-based nanoparticles dissociated, liberating approximately 50% of both active substances within 7 hours. ASOND-loaded polymer nanoparticles had higher stability and high mucoadhesive properties. The ASOND-loaded thiolated particles significantly suppressed EGFR gene expression in T47D cells compared with ASOND-loaded chitosan particles and downregulated EGFR protein expression in cells. This study could facilitate future investigations into the functionality of NAP-C and NAC-C polymers as an efficient ASOND delivery system in vitro and in vivo.

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“…Nanocarriers can be used to control permeation through the skin, and transport properties can be controlled independently of the cargo . Chitosan nanoparticles have been used for antisense oligonucleotide and plasmid DNA delivery to skin, with reporter gene effects persisting on rat skin for 6–7 days . Free nucleic acid had no measurable transfection, indicating that a drug silvery system is required to effectively protect and deliver topically applied nucleic acids.…”

Section: Tumor Targetingmentioning

confidence: 99%

Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

2019

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Nucleic acids are a promising type of therapeutic for the treatment of a wide range of conditions, including cancer, but they also pose many delivery challenges. For efficient and safe delivery to cancer cells, nucleic acids must generally be packaged into a vehicle, such as a nanoparticle, that will allow them to be taken up by the target cells and then released in the appropriate cellular compartment to function. As with other types of therapeutics, delivery vehicles for nucleic acids must also be designed to avoid unwanted side effects; thus, the ability of such carriers to target their cargo to cancer cells is crucial. Classes of nucleic acids, hurdles that must be overcome for effective intracellular delivery, types of nonviral nanomaterials used as delivery vehicles, and the different strategies that can be employed to target nucleic acid delivery specifically to tumor cells are discussed. Additonally, nanoparticle designs that facilitate multiplexed delivery of combinations of nucleic acids are reviewed.

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Topical Application of Antisense Oligonucleotide-Loaded Chitosan Nanoparticles to Rats

Cited by 29 publications

References 24 publications

Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

Thiolated chitosan nanoparticles as a delivery system for antisense therapy: evaluation against EGFR in T47D breast cancer cells

Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

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