Topical application of Chinese herbal medicine DAEP relieves the osteoarthritic knee pain in rats

Siu, Wing Sum; Shum, Wai Ting; Cheng, Weiguo; Wong, Chun Wai; Shiu, Hoi Ting; Ko, Chun‐Hay; Leung, Ping Chung; Lam, Christopher Wai Kei

doi:10.1186/s13020-019-0278-1

Cited by 17 publications

(14 citation statements)

References 42 publications

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“…Our findings revealed that 5-Loxin caused a significant reduction in inflammatory responses and cartilage degradation in aMIA-induced OA model in rats. One measure of OA in rats is reduced HWD due to joint pain and stiffness [ 29 ]. HWD assesses the distribution of the pressure exerted by each hind paw.…”

Section: Discussionmentioning

confidence: 99%

Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis

Shin

Kim

Choi

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Osteoarthritis (OA) is a chronic, progressive joint disease associated with pain, functional impairment, and diminished quality of life in affected individuals. At a societal level, it also has a high economic burden. Boswellia serrata has been reported to have potent anti-inflammatory, antiarthritic, and analgesic effects. The aim of this study was to explore the therapeutic potential and possible underlying mechanism of 5-Loxin®, a standardized Boswellia serrata extract, in a rat model of OA. The OA model was established by the intra-articular injection of 50 μL of monosodium iodoacetate (MIA) (60 mg/mL). 5-Loxin® was administered orally, and efficacy was evaluated through serum analysis, real-time polymerase chain reaction (PCR), histologic staining, and micro-computed tomography (micro-CT). Results indicated that administration of 5-Loxin® can relieve OA joint pain through inhibition of both inflammatory processes and cartilage degeneration. In the group of rats treated with 5-Loxin®, the suppression of inflammatory enzymes such as cyclooxygenase (COX)-2 and 5-lipoxygenase (LOX) resulted in a significant reduction in the prostaglandin (PG) E2 and leukotriene (LT) B4 levels. Moreover, 5-Loxin® ameliorated the deterioration of the main components of the articular extracellular matrix (ECM), such as glycosaminoglycans (GAGs) and aggrecan, through the downregulation of matrix metalloproteinases (MMPs). These findings suggest that 5-Loxin® may be a potential therapeutic agent for the treatment of OA.

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Section: Discussionmentioning

confidence: 99%

Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis

Shin

Kim

Choi

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Dipsacus asperoides, a traditional herbal medicine, has long been used in China and Korea for the treatment of bone diseases such as bone fracture, osteoporosis, and rheumatoid arthritis ( Wong et al, 2007 ; Gong et al, 2019 ). According to recent studies, Dipsacus asperoides has beneficial therapeutic effects on inflammation, osteoporosis, and joint disease ( Liu et al, 2016a ; Yue et al, 2017 ; He et al, 2019 ; Siu et al, 2019 ). However, the effects of Dipsacus asperoides on OA have not yet been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports show that Dipsacus asperoides has a substantial antirheumatic effect on collagen-induced arthritis in mice ( Jung et al, 2012 ) and an osteoprotective effect in ovariectomized rats ( He et al, 2019 ; Sun et al, 2019 ). In addition, Chinese herbal medicine paste containing Dipsacus asperoides relieves osteoarthritic knee pain in rats ( Siu et al, 2019 ); these effects are related to the ethnopharmacological actions of the plant. However, the ethnomedicinal effects of Dipsacus asperoides on the joint disease osteoarthritis (OA) have not yet been reported, even though natural product research for OA is an area of active research.…”

Section: Introductionmentioning

confidence: 99%

Effects of Dipsacus asperoides Extract on Monosodium Iodoacetate–Induced Osteoarthritis in Rats Based on Gene Expression Profiling

et al. 2021

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The root of Dipsacus asperoides C. Y. Cheng et T. M. Ai is traditionally used as an analgesic and anti-inflammatory agent to treat pain, rheumatoid arthritis, and bone fractures. However, neither its effects on osteoarthritis (OA) nor its effects on the arthritic cartilage tissue transcriptome have not been fully investigated. In this study, we used a rat model of monosodium iodoacetate- (MIA-) induced OA to investigate the therapeutic effects of a Dipsacus asperoides ethanolic extract (DAE, 200 mg/kg for 21 days). The study first assessed joint diameter, micro-CT scans, and histopathological analysis and then conducted gene expression profiling using RNA sequencing in articular cartilage tissue. We found that DAE treatment ameliorates OA disease phenotypes; it reduced the knee joint diameter and prevented changes in the structural and histological features of the joint, thereby showing that DAE has a protective effect against OA. Based on the results of gene expression profiling and subsequent pathway analysis, we found that several canonical pathways were linked to DAE treatment, including WNT/β-catenin signaling. Taken together, the present results suggest molecular mechanism, involving gene expression changes, by which DAE has a protective effect in a rat model of MIA-induced OA.

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“…For OA studies, Stand and Swing durations and Paw Print Area are commonly reported parameters measured by CatWalk in gait analysis [ 43 , 44 , 45 , 46 , 47 ]. For gait and pain sensitivity measurements, a biphasic response is reported in the literature, characterized by an early synovial inflammatory phase up to day 7, and a second chronic phase from day 14 corresponding to structural and degenerative alterations [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis

Tschon

Salamanna

Martini

et al. 2020

Cells

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The purpose of this study was to verify the efficacy of a single intra-articular (i.a.) injection of a hyaluronic acid-chitlac (HY-CTL) enriched with two low dosages of triamcinolone acetonide (TA, 2.0 mg/mL and 4.5 mg/mL), in comparison with HY-CTL alone, with a clinical control (TA 40 mg/mL) and with saline solution (NaCl) in an in vivo osteoarthritis (OA) model. Seven days after chemical induction of OA, 80 Sprague Dawley male rats were grouped into five arms (n = 16) and received a single i.a. injection of: 40 mg/mL TA, HY-CTL alone, HY-CTL with 2.0 mg/mL TA (RV2), HY-CTL with 4.5 mg/mL TA (RV4.5) and 0.9% NaCl. Pain sensitivity and Catwalk were performed at baseline and at 7, 14 and 21 days after the i.a. treatments. The histopathology of the joint, meniscus and synovial reaction, type II collagen expression and aggrecan expression were assessed 21 days after treatments. RV4.5 improved the local pain sensitivity in comparison with TA and NaCl. RV4.5 and TA exerted similar beneficial effects in all gait parameters. Histopathological analyses, measured by Osteoarthritis Research Society International (OARSI) and Kumar scores and by immunohistochemistry, evidenced that RV4.5 and TA reduced OA features in the same manner and showed a stronger type II collagen and aggrecan expression; both treatments reduced synovitis, as measured by Krenn score and, at the meniscus level, RV4.5 improved degenerative signs as evaluated by Pauli score. TA or RV4.5 treatments limited the local articular cartilage deterioration in knee OA with an improvement of the physical structure of articular cartilage, gait parameters, the sensitivity to local pain and a reduction of the synovial inflammation.

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Topical application of Chinese herbal medicine DAEP relieves the osteoarthritic knee pain in rats

Cited by 17 publications

References 42 publications

Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis

Boswellia serrata Extract, 5-Loxin®, Prevents Joint Pain and Cartilage Degeneration in a Rat Model of Osteoarthritis through Inhibition of Inflammatory Responses and Restoration of Matrix Homeostasis

Effects of Dipsacus asperoides Extract on Monosodium Iodoacetate–Induced Osteoarthritis in Rats Based on Gene Expression Profiling

Boosting the Intra-Articular Efficacy of Low Dose Corticosteroid through a Biopolymeric Matrix: An In Vivo Model of Osteoarthritis

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