Topical Application of Eosin to Burns Produces Interference in Measurement of Serum Vancomycin by Fluorescence Polarization Immunoassay

Leal, Teresinha; Dupret, Philippe; Hassoun, A.; Wallemacq, Pierre

doi:10.1093/clinchem/43.7.1238

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Cited by 4 publications

(2 citation statements)

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“…Eosin applied topically to burns patients has been shown to cause negative interference by causing very high blank readings. 2 Conversely, false elevations occur in renal failure patients and in pre-term infants due to cross-reactivity of the polyclonal antibody with vancomycin degradation products. 3,4 This interference does not occur in the AxSYM vancomycin assay.…”

Section: Short Reportmentioning

confidence: 99%

Falsely low vancomycin results using the Abbott TDx

Wood

Earl²,

Nath³

et al. 2000

Ann Clin Biochem

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Section: Short Reportmentioning

confidence: 99%

Falsely low vancomycin results using the Abbott TDx

Wood

Earl²,

Nath³

et al. 2000

Ann Clin Biochem

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“…Despite the clinical importance of vancomycin, few methods have been reported for its determination, whether in dosage forms or biological fluids. These methods include HPLC [2−6], fluorescence polarization immunoassay [7,8] and colorimetry [9].…”

Section: Introductionmentioning

confidence: 99%

Voltametric Determination of Vancomycin in Dosage Forms through Treatment with Nitrous Acid

Belal

El‐Ashry

El‐Kerdawy

et al. 2011

Arzneimittelforschung

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Two methods are described for the determination of vancomycin (vancomycin hydrochloride, CAS 1404-93-9) in its dosage forms. The two methods involve a prior treatment with nitrous acid then measuring the formed nitroso derivative, either spectrophotometrically or polarographically. In the spectrophotometric method, the absorbance-concentration plot is rectilinear over the range of 4-32 micrograms/ml with minimum detectability of 2.7 micrograms/ml (1.8 x 10(-6) mol/l). The apparent molar absorptivity is 4.084 x 10(-4)l.mol-1.cm-1 and A (1%, 1 cm) is 275. The reaction product was also found to be polarographically reducible at the Dropping Mercury Electrode (DME) with E1/2 of-0.9 V vs. Ag/AgCl electrode and a diffusion current constant (Id) of 0.85 +/- 0.02. The cathodic current produced was found to be diffusion controlled with some adsorption contribution. The calibration plot was linear over the range of 0.015-0.06 mmol l-1 for direct current (DCt) mode and from 0.005-0.05 mmol l-1 for differential pulse polarography (DPP) mode with minimum detectability of 2.4 x 10(-7) mol l-1 using the latter technique. The results obtained were statistically compared with those given with the official B.P. method and were in good agreement.

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Miscellaneous antibacterial drugs

Aronson¹

1999

Side Effects of Drugs Annual

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Topical Application of Eosin to Burns Produces Interference in Measurement of Serum Vancomycin by Fluorescence Polarization Immunoassay

Cited by 4 publications

References 8 publications

Falsely low vancomycin results using the Abbott TDx

Falsely low vancomycin results using the Abbott TDx

Voltametric Determination of Vancomycin in Dosage Forms through Treatment with Nitrous Acid

Miscellaneous antibacterial drugs

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