Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model

Abstract

This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 10 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.

Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers

“…Induction of infection wound was performed as we previously reported [1,2]. All mice were anaesthetized by intramuscular administration of ketamine/xylazine (ketamine 60 and xylazine 20 mg/kg) cocktail and then the skin was scrubbed by a shaver.…”

“…Following dilution, the samples were superficially cultured on plate count agar (Merck KGaA, Darmstadt, Germany) and then duplicated. Next, the culture plates were incubated at 37 C for 24 h and 48 h. Finally, all the colonies were counted and the data were reported as CFU/g of granulation tissue [1,2,13].…”

“…All parameters were analyzed in high-power fields (HPFs). The epithelial thickness was finally evaluated by a morphometric lens (Olympus, Tokyo, Japan) and reported in mm [1]. The collagen intensity was analyzed by using Image pro-insight software (version 9.00, Cyber media, USA) .…”

“…Colonization and replication of bacteria in the wound site delay the wound healing process. The use of topical antimicrobial agents such as silver sulfadiazine can decrease the risk of infection during wound healing; however, microbial resistance to antibiotics causes public health problems [1,2]. The use of natural antimicrobial agents that are isolated from the essential oils of plants can be an appropriate strategy for preventing the bacterial growth and promoting the wound healing process.…”

Antifungal and antibacterial treatments based on natural compounds for lining leather and footwear articles

“…They can be synthesized by all the organs of the plant, like buds, flowers, leaves, stems, twigs, seeds, fruits, roots, wood or bark and are kept in secretory cells, cavities, channels, epidermal cells or glandular trichomes [11]. The antifungal and antibacterial effects of the essential oils extracted from the plants can inhibit their growth by different mechanisms initiated by the constituent compounds [14,15]. Essential oils are complex mixtures of low molecular weight compounds extracted from plants by hydrodistillation and various solvents.…”