Topical formulations and wound healing applications of chitosan

Ueno, Hiroshi; Mori, Takashi; Fujinaga, Toru

doi:10.1016/s0169-409x(01)00189-2

Cited by 725 publications

(425 citation statements)

References 24 publications

Supporting

Mentioning

397

Contrasting

Unclassified

Order By: Relevance

“…25,26) Therefore, absorption capacity and swelling rate of the gels are crucial factors. [6][7][8] Fucoidan-chitosan gels differ in their water absorption capacity depending on polymer concentration and molecular weight of chitosan. Addition of fucoidan, more hydrophilic than chitosan, increased both the water absorption capacity and the swelling rate of the gels (pϽ0.05) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

160

View full text Add to dashboard Cite

Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

160

View full text Add to dashboard Cite

show abstract

“…25 Yaralara kitozan uygulanmasından sonra, histolojik olarak ciddi polimorfonükleer hücre infiltrasyonu, yara alanında makrofaj göçü ve yabancı dev hücreler görülmektedir. 26 Bu bulgular iyileşmenin erken evrelerinde kitozanın proinflamatuar ürünlerin üretimini ve salgılanma yeteneğini artırdığını göstermektedir. Makrofajlar yara iyileşmesini etkileyen büyüme faktörlerini sentezlerler.…”

unclassified

“…Kitozan fibroblast çoğalmasını artırır ve ekstrasellüler matriks üretimini direkt veya indirekt olarak uyarır. 26,27 Kitozan uygulamasını takiben granülasyon ve kollajen III miktarının arttığı görülmüştür. 26 Kitin ve kitozan tümör hücreleri ve patojenlere karşı immünolojik cevapta rol oynayan hücrelerin fonksiyonunu arttırır.…”

unclassified