Topical non-invasive gene delivery using gemini nanoparticles in interferon-γ-deficient mice

Badea, Ildikó; Wettig, Shawn D.; Verrall, R. E.; Földvári, Marianna

doi:10.1016/j.ejpb.2007.01.002

Cited by 85 publications

(66 citation statements)

References 39 publications

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“…In our preliminary study, 3-day TNP treatment produced similar IFN-g levels to those measured in the skin of CD1 animals treated topically for 3 days with the TNP formulation, 16 as well as by topical treatment of IFN-g-deficient mice 17 (results not shown). In the Tsk/ þ mice, after the 20-day treatment regimen, intradermal injections with plasmid solutions appeared to produce higher transgene expression (1069 ± 612 pg cm…”

Section: Resultsmentioning

confidence: 70%

“…We have previously shown that gemini surfactant-based selfassembled DNA nanoparticles (topical nanoparticles (TNPs)) deliver IFN-g-coding plasmid into keratinocytes in vitro and into skin of normal (CD1) mice, 16 as well as in IFN-g-deficient knockout mice. 17 The level of IFN-g gene expression in vivo was similar in the two animal models and was indicative of pharmacologically relevant levels. In this paper, our objective was to evaluate the delivery of plasmid DNA coding for IFN-g via intradermal injection and topical treatment (at a topical dose of 100 mg) with plasmid DNA coding for IFN-g encapsulated in gemini nanoparticles in Tsk/ þ mice.…”

Section: Introductionmentioning

confidence: 88%

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Effect of topical interferon-γ gene therapy using gemini nanoparticles on pathophysiological markers of cutaneous scleroderma in Tsk/+ mice

et al. 2011

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Scleroderma is a chronic disorder manifested by excessive synthesis and deposition of collagen in skin and connective tissue, vascular abnormalities, and autoimmunity. Using microarray and real-time PCR data, we show that intradermally expressed interferon g (IFN-g), generated after intradermal injection of IFN-g-coding plasmid, and non-invasive topical nanoparticle (TNP) treatment with IFN-g-coding plasmid, decreased collagen synthesis (via the Jak/Stat 1 pathway), upregulated Th1 cytokine levels, and downregulated the profibrotic cytokine Transforming growth factor b and the Smad pathways in the Tsk/ þ (tight-skin scleroderma) mouse model. The TNP gene delivery system was constructed from gemini surfactant 16-3-16 and IFN-g-coding plasmid. Topical administration of IFN-g-coding plasmid in TNPs was effective in expressing IFN-g levels after a 20-day treatment regimen without increased TLR4, CCL2, CCL11 and CCR2 mRNA levels that were observed in injected animals, signs considered to be innate responses to injury. The more uniform transgene IFN-g expression caused significant (70 --72%) collagen reduction, as assessed by reverse transcription real-time PCR. These results demonstrate efficient in vivo transfection using a gemini surfactant-based TNP delivery system able to modulate excessive collagen synthesis in scleroderma-affected skin.

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Section: Resultsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 88%

Effect of topical interferon-γ gene therapy using gemini nanoparticles on pathophysiological markers of cutaneous scleroderma in Tsk/+ mice

et al. 2011

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“…So they have promising applications in skin care, medicine, life science, petrochemistry, construction of porous materials, etc. [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and properties of ionic liquid-type Gemini imidazolium surfactants

Zhu

et al. 2008

Journal of Colloid and Interface Science

210

134

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“…Cationic gemini surfactants are made up of spacer linked with two cationic heads connected to hydrocarbon tails individually. So developed γ-IFN gene-loaded nanoparticles indicated better plasmid packing and lack of irritancy as compared to simple cationic lipid nanoparticles and further showed signifi cant enhancement in gene expression as compared to untreated controlled, naked gene treatment when studied in γ-IFN-defi cient mouse model [ 93 ]. Additionally, liquid crystalline phase nanodispersions comprising of monoolein with cationic polyethylenimine polymer and oleylamine lipid were investigated as nonviral vectors for delivery of siRNA wherein the nanodispersion indicated skin penetration up to viable epidermis with marked reduction in target protein expression.…”

Section: Miscellaneous Nanotherapeuticsmentioning

confidence: 96%