Topical Treatment of Second-Degree Burn Wounds with Lactobacillus plantarum Supernatant: Phase I Trial

Abstract

Scan to discover onlineBackground & Objective: A burn wound is sterile immediately after injury, but opportunistic bacteria colonize the wound within 48 to 72 hours after the burn, causing delayed or failed burn wound healing. In addition, the presence of multidrug-resistant (MDR) pathogens doubles the treatment problems. Lactobacillus plantarum (L. plantarum) is a well-known antibacterial and healing agent that could be used topically to treat burn wounds. Case Series Presentation:This clinical trial study (Case Series) was performed on 20 patients with deep second-degree burns. Patients had bilateral wounds; the wound on one side of the body was considered as control (treated with silver sulfadiazine) and the other side of the body as treatment (treated with bacteria-free supernatants (BFS) of L. plantarum). The wounds were evaluated by microbial assessments and assessments related to healing. Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus aureus were isolated from 4 (22.2%), 0%, and 2 (11.1%) of wounds treated with L. plantarum on the fifth day of the treatment, respectively. Furthermore, 12 (66.7%) of wounds treated with L. plantarum were free from bacteria. The need for skin grafting was the same in both treatment and control groups, but graft rejection in the group treated with L. plantarum was (0%) (P=0.02). Conclusion:Regarding eliminating or reducing infection and wound healing, bacteria-free supernatants of L. plantarum can be considered a possible topical treatment option in the case of second-degree burn wounds.

Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from Lacticaseibacillus rhamnosus against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions

“…On the other hand, studies in animal models have demonstrated that the topical acidification of wounds results in beneficial effects promoting epithelialization, collagen deposition, and healing, with best outcomes observed with solutions at pH 3.5-4.0 [42,43]. For all these reasons, the results obtained herein support the therapeutic use of acidic L. rhamnosus GG CFS in wound care, providing experimental evidence for the encouraging results obtained in few clinical trials that used topical lactobacilli or their supernatants for the management of infected wounds [25,44].…”

Lactobacillus spp. na vanguarda: abordagem terapêutica não-medicamentosa no combate efetivo à Pseudomonas aeruginosa

“…Verificou-se que o uso desses pós-bióticos, além de ajudar no processo de cicatrização, diminuíram o surgimento de resistência em comparação ao uso com antibióticos. 44 Em conjunto, os trabalhos analisados evidenciam que os lactobacilos apresentam um potencial terapêutico, podendo ser utilizados em condutas clínicas, principalmente por não acelerar a resistência antimicrobiana e por favorecerem a diminuição dos efeitos negativos causados por patógenos.…”

“…Além disso, NaBut dispõe de múltiplas propriedades, uma delas é ser um inibidor histona deacetilase, podendo alterar a conformação da cromatina. 44 Testes in vitro evidenciaram um papel inibidor de crescimento de determinados micro-organismos, por meio da metodologia de Concentração Inibitória Mínima (CIM). Butirato de sódio também conseguiu inibir a formação de biofilmes de algumas espécies de micro-organismos patogênicos.…”

“…Butirato de sódio também conseguiu inibir a formação de biofilmes de algumas espécies de micro-organismos patogênicos. 44 Muito embora ainda não haja dados sobre o efeito de NaBut sobre crescimento de P.…”

Antibacterial Activity and Toxicity of Zinc Oxide Nanoparticles Combined with Supernatants of Lactobacillus spp. Against ESKAPE Bacteria: A Novel Mixture

“…Nowadays, bacteriotherapy is used as one of the new alternatives against pathogenic organisms. In this method, probiotics, as beneficial and safe bacteria, are often used with or without their supernatant to treat or prevent infections ( 4 , 5 ). Since there is no clear standard for applying probiotic formulations for antimicrobial therapy, it is needed to address existing gaps in this field.…”