Topical Vaccination of DNA Antigens: Topical Delivery of DNA Antigens

Choi, Myeong Jun; Maibach, Howard I.

doi:10.1159/000072067

Cited by 37 publications

(20 citation statements)

References 53 publications

(73 reference statements)

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“…In contrast, topical application of this vaccine with stripping induced strong antibody responses and elicited substantial CTL responses. There was a significant difference between the results of topical application with and without stripping [27,52].…”

Section: Tape Stripping and Topical Vaccinationmentioning

confidence: 84%

“…Skin accessibility makes it an easy target for vaccination. Thus, skin is an attractive target site for topical vaccination and has become the focus of intense study for the induction of antigen-specific immune responses [51,52]. Wang et al [53] observed that protein penetrates the SC barrier following occlusion by patch application, but im-Choi/Zhai/Löffler/Dreher/Maibach Cervical lymph node cells (effectors) obtained from mice immunized 10 days earlier with tyrosinase-related protein 2 peptide (VYDFFVWL, 96 Ìg/mouse) either through intact earlobes or earlobes tape stripped 12 h earlier were subjected to CTL assay using L kb target cells pulsed with tyrosinase peptide.…”

Section: Tape Stripping and Topical Vaccinationmentioning

confidence: 99%

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Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

et al. 2003

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The stratum corneum provides the first barrier to the percutaneous absorption of drugs as well as regulating water loss. This barrier limits the topical/transdermal delivery of drugs and biological macromolecules. Chemical and physical approaches have been examined to decrease these properties. Tape stripping is commonly used to disrupt the epidermal barrier, to enhance the delivery of drugs and to obtain information about stratum corneum function. Tape stripping results in the production and release of cytokines and co-stimulatory molecules and increases the humoral and cellular immune responses against peptide, protein and DNA antigens by a topical vaccination in vivo. This paper reviews the stripping method, experimental factors and its applications for penetration and topical vaccination.

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Section: Tape Stripping and Topical Vaccinationmentioning

confidence: 84%

Section: Tape Stripping and Topical Vaccinationmentioning

confidence: 99%

Effect of Tape Stripping on Percutaneous Penetration and Topical Vaccination

et al. 2003

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“…This barrier disruption process is painless, highly effective in augmenting the transfer of macromolecules such as proteins or DNA into skin, and has been successfully utilized in human clinical trials. 46,[48][49][50] An additional advantage is that physical barrier disruption itself has been shown to trigger activation of keratinocytes and LCs in skin, providing some level of 'built-in' adjuvant response. [44][45][46][49][50][51][52] We thus tested a model where the dorsal ears of anesthetized mice were tape-stripped followed by application of as-assembled, airdried (Poly-1/ova) 40 films on either glass or PDMS substrates, fixed in place by surgical tape against the exposed ear skin, mimicking a common strategy used in skin vaccination studies 45,46 (Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

Layer-by-Layer-Assembled Multilayer Films for Transcutaneous Drug and Vaccine Delivery

Su¹,

Kim²,

Kim³

et al. 2009

ACS Nano

156

120

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We describe protein-and oligonucleotide-loaded layer-by-layer (LbL)-assembled multiplayer films incorporating a hydrolytically degradable polymer for transcutaneous drug or vaccine delivery. Films were constructed based on electrostatic interactions between a cationic poly(β-amino ester) (denoted Poly-1) with a model protein antigen, ovalbumin (ova), and/or immunostimulatory CpG (Cytosine-phosphate diester-Guanine rich) DNA oligonucleotide adjuvant molecules. Linear growth of nanoscale Poly-1/ova bilayers was observed. Dried ova protein-loaded films rapidly deconstructed when rehydrated in saline solutions, releasing ova as non-aggregated/non-degraded protein, suggesting that the structure of biomolecules integrated into these multilayer films are preserved during release. Using confocal fluorescence microscopy and an in vivo murine ear skin model, we demonstrated delivery of ova from LbL films into barrierdisrupted skin, uptake of the protein by skin-resident antigen-presenting cells (Langerhans cells), and transport of the antigen to the skin-draining lymph nodes. Dual incorporation of ova and CpG oligonucleotides into the nanolayers of LbL films enabled dual release of the antigen and adjuvant with distinct kinetics for each component; ova was rapidly released while CpG was released in a relatively sustained manner. Applied as skin patches, these films delivered ova and CpG to Langerhans Cells in the skin. To our knowledge, this is the first demonstration of LbL films applied for the delivery of biomolecules into skin. This approach provides a new route for storage of vaccines and other immunotherapeutics in a solid-state thin film for subsequent delivery into the immunologically-rich milieu of the skin.

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“…In an in vivo mouse model, the application of pEGFP-N1 DNA led to the generation of GFP-specific antibodies. The topical spray application of pEGFP-N1 liposomal DNA formulations is a suitable method for plasmid DNA delivery to the skin, yielding significant gene expression [98,99]. Skin is an active immune surveillance site and shows both specific (immunity) and non-specific (inflammation) response for topical DNA vaccine [100].…”

Section: Topical Route For Dna Immunizationmentioning

confidence: 99%