2001
DOI: 10.1046/j.0022-202x.2001.01362.x
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Topically Applied Vitamin C Enhances the mRNA Level of Collagens I and III, Their Processing Enzymes and Tissue Inhibitor of Matrix Metalloproteinase 1 in the Human Dermis11Part of this work was presented in poster form at the American Academy of Dermatology, San Francisco, CA, March 10–15, 2000.

Abstract: Ascorbic acid (vitamin C) is a cofactor required for the function of several hydroxylases and monooxygenases. It is not synthesized in humans and some other animal species and has to be provided by diet or pharmacologic means. Its absence is responsible for scurvy, a condition related in its initial phases to a defective synthesis of collagen by the reduced function of prolylhydroxylase and production of collagen polypeptides lacking hydroxyproline, therefore, they are unable to assemble into stable triple-hel… Show more

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Cited by 246 publications
(179 citation statements)
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“…This temporal sequence of differentiation can also be described by changes in gene expression as we have previously shown (Birnbaum & Wiren 1994), where collagen mRNA abundance was high during proliferation, then declined; osteopontin expression was elevated during both the proliferation and mineralization; alkaline phosphatase increased as proliferation slowed; and osteocalcin expression increased during the mineralization stage, then declined in postmineralization cultures. This temporal sequence of differentiation of osteoblastic cultures does not proceed normally unless differentiation medium containing ascorbic acid is added , which can regulate transcription and post-transcriptional and post-translational processing of collagen (Nusgens et al 2001).…”
Section: Characterization Of In Vitro Differentiation In the Normal Rmentioning
confidence: 99%
“…This temporal sequence of differentiation can also be described by changes in gene expression as we have previously shown (Birnbaum & Wiren 1994), where collagen mRNA abundance was high during proliferation, then declined; osteopontin expression was elevated during both the proliferation and mineralization; alkaline phosphatase increased as proliferation slowed; and osteocalcin expression increased during the mineralization stage, then declined in postmineralization cultures. This temporal sequence of differentiation of osteoblastic cultures does not proceed normally unless differentiation medium containing ascorbic acid is added , which can regulate transcription and post-transcriptional and post-translational processing of collagen (Nusgens et al 2001).…”
Section: Characterization Of In Vitro Differentiation In the Normal Rmentioning
confidence: 99%
“…RT-PCR were performed to measure 28S rRNA, K16, VEGF-A, VEGF-C, VEGFR2, VEGFR3, NRP-1, NRP-2a, prox-1, PlGF-1 and -2 mRNA, using a Superscript II Reverse Transcriptase (Invitrogen, Merelbeke, Belgium) and Takara Taq polymerase (Biomedical group, Shiga, Japan). RT-PCR amplifications were performed in an automated thermal cycler (GeneAmp PCR System 9700, Applied Biosystems, Foster City, USA) with different pairs of primers as previously described [10,27,28]. For VEGF-A and PlGF, forward and reverse primers were chosen in regions surrounding the alternatively skipped sequences, allowing the discrimination of the various splice variants on the basis of the size of their amplification product [29].…”
Section: Rna Isolation and Rt-pcrmentioning
confidence: 99%
“…This quantitative assay allows a comparative analysis of samples, displaying an extended range in expression level. The accuracy, linearity of response and reliability of this procedure have been reported previously (14,15).…”
Section: Quantitative Rt-pcrmentioning
confidence: 99%
“…Total RNA was extracted and purified by ultracentrifugation on a cesium chloride cushion (13) from snap frozen biopsy samples collected from six volunteers as described previously (14). Specific mRNA and 28S ribosomal RNA (rRNA) were measured by quantitative noncompetitive RT-PCR.…”
Section: Quantitative Rt-pcrmentioning
confidence: 99%
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